Publications de Jean-Manuel Segura

Site-selective template-directed synthesis of antibody Fc conjugates with concomitant ligand release

Jean-Manuel Segura, David Viertl, Natalia Gasilova

Template-directed methods are emerging as some of the most effective means to conjugate payloads at selective sites of monoclonal antibodies (mAbs). We have previously reported a method based on an engineered Fc-III reactive peptide to conjugate a radionuc ...

Royal Soc Chemistry2023

Acetylcholine Receptor Organization in Membrane Domains in Muscle Cells: EVIDENCE FOR RAPSYN-INDEPENDENT AND RAPSYN-DEPENDENT MECHANISMS

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Joachim Piguet, Christoph Schreiter

Nicotinic acetylcholine receptors (nAChR) in muscle fibers are densely packed in the postsynaptic region at the neuromuscular junction. Rapsyn plays a central role in directing and clustering nAChR during cellular differentiation and neuromuscular junction ...

American Society for Biochemistry and Molecular Biology2011

Increased Mobility of Major Histocompatibility Complex I-Peptide Complexes Decreases the Sensitivity of Antigen Recognition

Horst Vogel, Jean-Manuel Segura

American Society for Biochemistry and Molecular Biology2008

Repetitive Reversible Labeling of Proteins at Polyhistidine Sequences for Single-Molecule Imaging in Live Cells

Rudolf Hovius, Horst Vogel, Emmanuel Guignet, Jean-Manuel Segura

Sensitive live-cell fluorescence microscopy and single-molecule imaging are severely limited by rapid photobleaching of fluorescent probes. Herein, we show how to circumvent this problem using a novel, generic labeling strategy. Small nickel-nitrilotriacet ...

2007

Visualizing odorant receptor trafficking in living cells down to the single-molecule level

Horst Vogel, Horst Pick, Valérie Jacquier, Jean-Manuel Segura

Despite the importance of trafficking for regulating G protein-coupled receptor signaling, for many members of the seven transmembrane helix protein family, such as odorant receptors, little is known about this process in live cells. Here, the complete lif ...

National Academy of Sciences2006

Supported cell-membrane sheets for functional fluorescence imaging of membrane proteins

Jean-Baptiste Perez, Horst Vogel, Jean-Manuel Segura

Cell-membrane sheets suitable for in-vitro functional fluorescence studies have been prepd. by direct detachment from cell membranes using poly-L-lysine-coated glass slides. The resulting transferred planar membranes conserve the compn. as well as most pro ...

2006

Determining a receptor-ligand interaction useful in e.g. drug screening involves repetitive application of reversibly binding fluorescent ligands with fast association-dissociation kinetics

Rudolf Hovius, Horst Vogel, Marinela Gjoni Boillat, Jean-Manuel Segura, Christoph Schreiter

NOVELTY - Determining a receptor-ligand interaction involves repeatedly contacting a cell comprising a receptor, with a ligand, of the receptor in which the ligand has a fluorescence labeling group and binds reversibly to the receptor under conditions in w ...

2006

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Jean-Baptiste Perez, Horst Vogel, Jean-Manuel Segura, Joachim Piguet

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

Elsevier2006

FRET imaging reveals that functional neurokinin-1 receptors are monomeric and reside in membrane microdomains of live cells

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Kai Johnsson, Nathalie George

The lateral organization of a prototypical G protein-coupled receptor, the neurokinin-1 receptor (NK1R), was investigated in living cells by fluorescence resonance energy transfer (FRET) microscopy, taking advantage of the recently developed acyl carrier p ...

National Academy of Sciences2006

Posttranslational covalent labeling reveals heterogeneous mobility of individual G protein-coupled receptors in living cells

Horst Vogel, Jean-Manuel Segura, Kai Johnsson, Bruno Meyer, Raphael Franzini, Nathalie George

2006

Covalent labeling of cell-surface proteins for in-vivo FRET studies

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Kai Johnsson, Bruno Meyer, Nathalie George, Pedro Pascoal

Fluorescence resonance energy transfer (FRET) is a powerful technique to reveal interactions between membrane proteins in live cells. Fluorescence labeling for FRET is typically performed by fusion with fluorescent proteins (FP) with the drawbacks of a lim ...

2006

CD8+ Cytotoxic T Lymphocyte Activation by Soluble Major Histocompatibility Complex-Peptide Dimers

Horst Vogel, Jean-Manuel Segura, Bruno Meyer, Olivier Michielin

CD8+ cytotoxic T lymphocyte (CTL) can recognize and kill target cells that express only a few cognate major histocompatibility complex class I-peptide (pMHC) complexes. To better understand the mol. basis of this sensitive recognition process, the authors ...

2005

Synthesis of nanoscopic optical fibers using lipid membranes as templates

Rudolf Hovius, Pierre Stadelmann, Horst Vogel, Dimitrios Stamou, Marinela Gjoni Boillat, Jean-Manuel Segura, Gopakumar Gopalakrishnan, Kurt Schenk, Cédric Gaillard

High-aspect-ratio, monocryst. Cd chloride nanowires doped with fluorescent CdS nanoparticles were synthesized using planar lipid membranes as templates. Cd2+ ions bound to the lipid bilayers (a) are released at low pH to the nanometer-sized, interlamellar ...

Wiley-VCH Verlag GmbH2005

Reversible sequential-binding probe receptor-ligand interactions in single cells

Rudolf Hovius, Horst Vogel, Marinela Gjoni Boillat, Jean-Manuel Segura, Christoph Schreiter

With the reversible sequential (ReSeq) binding assay, we present a novel approach for the ultrasensitive profiling of receptor function in single living cells. This assay is based on the repetitive application of fluorescent ligands that have fast assocn.- ...

2005