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Lucia Baldi Unser

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Publications associées (55)

DNA delivery with hyperbranched polylysine: A comparative study with linear and dendritic polylysine

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Harm-Anton Klok, Zuzana Kadlecova, Yashas Rajendra

PEI and polylysine are among the most investigated synthetic polymeric carriers for DNA delivery. Apart from their practical use, these 2 classes of polymers are also of interest from a fundamental point of view as they both can be prepared in different ar ...

Elsevier2013

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, Simone Schmitt, Yury Tsybin, Luca Fornelli, Julien Parra

In this study, a recombinant monoclonal IgG antibody was produced by transient gene expression (TGE) in suspension-adapted HEK-293E cells. The objective of the study was to determine the variation in recombinant IgG yield and glycosylation in ten independe ...

Elsevier2012

Comparative Study on the In Vitro Cytotoxicity of Linear, Dendritic, and Hyperbranched Polylysine Analogues

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Harm-Anton Klok, Zuzana Kadlecova

Lysine-based polycations are widely used as nonviral carriers for gene delivery. This manuscript reports the results of a comparative study on the in vitro cytotoxicity of a library of three structural polylysine variants, namely, linear polylysine (LPL), ...

American Chemical Society2012

A NanoDrop-based method for rapid determination of viability decline in suspension cultures of animal cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Mélanie Hubert

We describe a rapid method for monitoring the cell growth and decline phases in suspension cultures of animal cells. During the cell growth phase, ultraviolet (UV)-absorbing components in the medium are consumed, but at later times as cells begin to die, U ...

Elsevier2012

Hyperbranched Polylysine: A Versatile, Biodegradable Transfection Agent for the Production of Recombinant Proteins by Transient Gene Expression and the Transfection of Primary Cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Harm-Anton Klok, Zuzana Kadlecova, Yashas Rajendra

The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in I ...

2012

Role of non-specific DNA in reducing coding DNA requirement for transient gene expression with CHO and HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sagar Shashidhar Manoli

Transient gene expression (TGE) is a rapid method for the production of recombinant proteins in mammalian cells. While the TGE volumetric productivity has improved significantly over the past decade, the amount of plasmid DNA (pDNA) needed for transfection ...

2012

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Stéphanie Tissot

The aim of this study was to gain a better understanding of orbitally shaken bioreactors (OSRs) operated without controllers for pH and dissolved oxygen (DO) concentration. We used cylindrical OSRs with working volumes ranging from 250mL to 200L to determi ...

Elsevier2012

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker, Harm-Anton Klok, Zuzana Kadlecova

Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI Max) regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI ...

Wiley-Blackwell2012

Large-Scale Transfection of Mammalian Cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Carine Meerschman

The large-scale transfection of mammalian cells allows moderate (milligram to gram) amounts of recombinant proteins (r-proteins) to be obtained for fundamental or clinical research. In this article, we describe a one-liter transfection using polyethyleneim ...

Humana Press2011

Hydrodynamic stress in orbitally shaken bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Alfio Quarteroni, Mohamed Farhat, Marco Discacciati, David Hacker, Stéphanie Tissot, Martino Reclari, Samuel Quinodoz, Matthieu Dreyer, Dominique Terry Monteil Jr

2011

A Special Issue on Nanoengineered Silicon: Technology and Applications

Lucia Baldi Unser, Sandro Carrara

2011

Influence of glutamine on transient and stable recombinant protein production in CHO and HEK-293 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

2011

The use of filler DNA for improved transfection and reduced DNA needs in transient gene expression with CHO and HEK cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra, Sagar Shashidhar Manoli

2011

Transient gene expression with CHO cells in conditioned medium: a study using TubeSpin® bioreactors

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Yashas Rajendra, Joao Nuno Dos Santos Pereira

2011

Rapid recombinant protein production from pools of transposon-generated CHO cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann

2011

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Florian Maria Wurm, Lucia Baldi Unser, Mohamed Farhat, David Hacker, Stéphanie Tissot, Martino Reclari, Matthieu Dreyer, Agata Oberbek

Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are ...

Elsevier2011

The PiggyBac transposon enhances the frequency of CHO stable cell line generation and yields recombinant lines with superior productivity and stability

Florian Maria Wurm, Lucia Baldi Unser, David Hacker

Generating stable, high-producing mammalian cell lines is a major bottleneck in the manufacture of recombinant therapeutic proteins. Conventional gene transfer methods for cell line generation rely on random plasmid integration, resulting in unpredictable ...

Wiley-Blackwell2011

CHO cell lines generated by PiggyBac transposition

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann

2011

A simple high-yielding process for transient gene expression in CHO cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Divor Kiseljak, Yashas Rajendra

Here we describe a simplified method for transient gene expression (TGE) in suspension-adapted Chinese hamster ovary (CHO) cells using polyethylenimine (PEI) for DNA delivery. Both the transfection and production phases of the bioprocess were performed at ...

Elsevier2011

TubeSpin bioreactor 50 for the high-density cultivation of Sf-9 insect cells in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiaowei Zhang, Qiuling Xie

Here we present the TubeSpin bioreactor 50 (TubeSpins) as a simple and disposable culture system for Sf-9 insect cells in suspension. Sf-9 cells had substantially better growth in TubeSpins than in spinner flasks. After inoculation with 106 cells/ml, maxim ...

Springer Netherlands2011

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