Publications de Florian Charrière

Method and apparatus to measure and compute the amplitude point spread function and associated parameters of a coherent optical imaging system

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Mihaela Marian

The invention concerns a method of and an apparatus for the measurement of the Amplitude Point Spread Function (APSF), of an Optical Imaging System (OIS), wherein the APSF is defined as the complex field distribution of the beam delivered by the OIS and or ...

2008

Simultaneous cell morphometry and refractive index measurement with dual- wavelength digital holographic microscopy and dye-enhanced dispersion of perfusion medium

Christian Depeursinge, Pierre Marquet, Florian Charrière, Pierre Magistretti, Benjamin Rappaz

Digital holographic microscopy (DHM) allows optical-path-difference (OPD) measurements with nanometric accuracy. OPD induced by transparent cells depends on both the refractive index (RI) of cells and their morphology. This Letter presents a dual-wavelengt ...

2008

Axial sub-nanometer accuracy in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

2008

On the complex three-dimensional amplitude point spread function of lenses and microscope objectives: theoretical aspects, simulations and measurements by digital holography

Tristan Colomb, Frédéric Montfort, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

The point spread function is widely used to characterize the three-dimensional imaging capabilities of an optical system. Usually, attention is paid only to the intensity point spread function, whereas the phase point spread function is most often neglecte ...

2007

Real-time dual-wavelength digital holographic microscopy with a single hologram acquisition

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

A technique to perform two-wavelengths digital holographic microscopy (DHM) measurements with a single hologram acquisition is presented. The vertical measurement range without phase ambiguity is extended to the micron-range, thanks to the resulting synthe ...

2007

Amplitude point spread function measurement of high NA microscope objectives by digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Mihaela Marian

We present here a three-dimensional evaluation of the amplitude point-spread function (APSF) of a microscope objective (MO), based on a single holographic acquisition of its pupil wavefront. The aberration function is extracted from this pupil measurements ...

2007

Influence of shot noise on phase measurement accuracy in digital holographic microscopy

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital Holographic Microscopy (DHM) is a single shot interferometric technique, which provides quantitative phase images with subwavelength axial accuracy. A short hologram acquisition time (down to microseconds), allows DHM to offer a reduced sensitivity ...

2007

Digital holographic microscopy applied to life sciences

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Benjamin Rappaz

Digital holographic microscopy (DHM) is applied to life sciences applications and demonstrate its capability of real-time imaging and quantitative measurements of physiological parameters such as cell volume or mean cell hemoglobin concentration (MCHC) of ...

IEEE2007

Shot noise, refractive index tomography and aberrations estimation in digital holographic microscopy

Florian Charrière

The present thesis develops some specific aspects of digital holographic microscopy (DHM), namely the effect of shot noise on the phase image accuracy, the use of DHM in micro-tomography and in aberrations evaluation of a microscope objective (MO). DHM is ...

EPFL2007

WAVE FRONT SENSING METHOD AND APPARATUS

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Florian Charrière, Mihaela Marian

A new way of mixing instrumental and digital means is described for the general field of wave front sensing. The present invention describes the use, the definition and the utility of digital operators, called digital wave front operators (DWFO) or ... A n ...

2006

Cell refractive index tomography by digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Mihaela Marian

For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired ...

2006

Total aberrations compensation in digital holographic microscopy with a reference conjugated hologram

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière

In this paper we present a new method to achieve quantitative phase contrast imaging in Digital Holographic Microscopy (DHM) that allows to compensate for phase aberrations and image distortion by recording of a single reference hologram.We demonstrate tha ...

2006

Automatic procedure for aberration compensation in digital holographic microscopy and applications to specimen shape compensation

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière

We present a procedure that compensates for phase aberrations in digital holographic microscopy by computing a polynomial phase mask directly from the hologram. The phase-mask parameters are computed automatically without knowledge of physical values such ...

2006

Numerical parametric lens for shifting, magnification and complete aberration compensation in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Stéphane Bourquin, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Nicolas Aspert, Jonas Kühn, Florian Charrière, Mihaela Marian

The concept of numerical parametric lenses (NPL) is introduced to achieve wavefront reconstruction in digital holography. It is shown that operations usually performed by optical components and described in ray geometrical optics, such as image shifting, m ...

2006

Living specimen tomography by digital holographic microscopy: morphometry of testate amoeba

Tristan Colomb, Christian Depeursinge, Pierre Marquet, Florian Charrière, Nicolas Pavillon, Edward Mitchell, Benjamin Rappaz, Thierry Jean Heger

This paper presents an optical diffraction tomography technique based on digital holographic microscopy. Quantitative 2-dimensional phase images are acquired for regularly-spaced angular positions of the specimen covering a total angle of π, allowing to bu ...

2006

Characterization of microlenses by digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière

We demonstrate the use of digital holographic microscopy (DHM) as a metrological tool in micro-optics testing. Measurement principles are compared with those performed with Twyman-Green, Mach-Zehnder, and white-light interferometers. Measurements performed ...

2006

Shot noise influence in reconstructed phase image SNR in digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

In digital holographic microscopy, shot noise is an intrinsic part of the recording process with the digital camera. We present a study based on simulations and real measurements describing the shot-noise influence in the quality of the reconstructed phase ...

2006

Purely numerical compensation for microscope objective phase curvature in digital holographic microscopy: influence of digital phase mask position

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

Introducing a microscope objective in an interferometric setup induces a phase curvature on the resulting wavefront. In digital holography, the compensation of this curvature is often done by introducing an identical curvature in the reference arm and the ...

2006

Submicrometer optical tomography by multiple-wavelength digital holographic microscopy

Tristan Colomb, Frédéric Montfort, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Jonas Kühn, Florian Charrière, Sylvain Herminjard

We present a method for submicrometer tomographic imaging using multiple wavelengths in digital holographic microscopy. This method is based on the recording, at different wavelengths equally separated in the k domain, in off- axis geometry, of the interfe ...

2006

Time-domain optical coherence tomography with digital holographic microscopy

Pia Massatsch, Etienne Cuche, Christian Depeursinge, Pierre Marquet, Florian Charrière

We show that digital holography can be combined easily with optical coherence tomography approach. Varying the reference path length is the means used to acquire a series of holograms at different depths, providing after reconstruction images of slices at ...

2005