Publications de Mathieu Benjamin Stettler

Using a model of human visual perception to improve deep learning

2018

Lactate metabolism shift in CHO cell culture: the role of mitochondrial oxidative activity

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Francesca Zagari

Lactate production is monitored in industrial processes as a crucial metabolite for cultured mammalian cells. Typically lactate is strongly produced during the exponential growth phase, while its net consumption is frequently observed when cells enter into ...

Elsevier2013

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler, David Hacker, Stéphanie Tissot, Gilles Broccard, Xiaowei Zhang

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Schweizerische Chemische Gesellschaft2010

Efficient oxygen transfer by surface aeration in shaken cylindrical containers for mammalian cell cultivation at volumetric scales up to 1,000 liters

Florian Maria Wurm, Alfio Quarteroni, Mathieu Benjamin Stettler, Marco Discacciati, Nicola Parolini, David Hacker, Xiuwei Zhang, Marco Perrone, Dario De Sanctis

Cylindrical containers agitated by orbital shaking are being developed as simple and cost-effective bioreactor systems for the cultivation of mammalian cells. Here the oxygen transfer capacities of containers with nominal volumes from 50 mL to 2000 L were ...

2009

Use of Orbital Shaken Disposable Bioreactors for Mammalian Cell Cultures from the Milliliter-Scale to the 1,000-Liter Scale

Florian Maria Wurm, Alfio Quarteroni, Mathieu Benjamin Stettler, David Hacker, Xi Zhang

Driven by the commercial success of recombinant biopharmaceuticals, there is an increasing demand for novel mammalian cell culture bioreactor systems for the rapid production of biologicals that require mammalian protein processing. Recently, orbitally sha ...

2009

Bioreactor processes based on disposable materials for the production of recombinant proteins from mammalian cells

Mathieu Benjamin Stettler

The objective of industrial cell culture technology is to produce high value-added therapeutic proteins that are well suited for the treatment of infectious diseases, cancer, and autoimmune diseases. Nowadays, an important focus is the reduction of the tim ...

EPFL2007

High-titer, serum-free production of adeno-associated virus vectors by polyethyleneimine-mediated plasmid transfection in mammalian suspension cells

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler

Adeno-associated virus (AAV)-based vectors belong to the most promising gene transfer vectors in clinical studies. To provide vector for late-stage clinical trials as well as for a potential commercial phase, a scalable, cGMP-compliant process is required. ...

Springer, Springer Netherlands2007

Microfabricated solenoids and Helmholtz coils for NMR spectroscopy of mammalian cells

Pierre-André Besse, Radivoje Popovic, Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, Franck Vincent, Klaus Ehrmann

NMR-microprobes based on solenoids and Helmholtz coils have been microfabricated and NMR-spectra of mammalian cells have successfully been taken. The microfabrication technology developed for these probes consists of three electroplated copper levels for l ...

2007

Novel orbital shake bioreactors for transient production of CHO derived IgGs

Florian Maria Wurm, Mathieu Benjamin Stettler, David Hacker, Xiaowei Zhang

Large-scale transient gene expression in mammalian cells is being developed for the rapid production of recombinant proteins for biochemical and preclinical studies. Here, the scalability of transient production of a recombinant human antibody in Chinese h ...

American Chemical Society2007

NMR spectroscopy and perfusion of mammalian cells using surface microprobes

Pierre-André Besse, Radivoje Popovic, Florian Maria Wurm, Mathieu Benjamin Stettler, Jürgen Brugger, Klaus Ehrmann, Kristopher Pataky

NMR spectra of mammalian cells are taken using surface microprobes that are based on microfabricated planar coils. The surface microprobe resembles a miniaturized Petri dish commonly used in biological research. The diameter of the planar coils is 1 mm. Ch ...

2007

New disposable tubes for rapid and precise biomass assessment for suspension cultures of mammalian cells

Florian Maria Wurm, Martin Jordan, Mathieu Benjamin Stettler, David Hacker, Nicolas Jaccard

We present a new approach for biomass assessment in cell culture using a disposable microcentrifuge tube. The specially designed tube is fitted with an upper chamber for sample loading and a lower 5 microL capillary for cell collection during centrifugatio ...

2006