Publications de Giovanni Dietler

An Optical Fiber-Based Nanomotion Sensor for Rapid Antibiotic and Antifungal Susceptibility Tests

Giovanni Dietler, Sandor Kasas, Serguei Sekatski, Anne-Céline Verenna Kohler, Jiangtao Zhou, María Inés Villalba, Leonardo Venturelli

The emergence of antibiotic and antifungal resistant microorganisms represents nowadays a major public health issue that might push humanity into a post-antibiotic/antifungal era. One of the approaches to avoid such a catastrophe is to advance rapid antibi ...

Amer Chemical Soc2024

Hierarchical Protofilament Intertwining Rules the Formation of Mixed‐Curvature Amyloid Polymorphs

Henning Paul-Julius Stahlberg, Giovanni Dietler, Serguei Sekatski, Francesco Simone Ruggeri, Jiangtao Zhou, Meltem Tatli

Amyloid polymorphism is a hallmark of almost all amyloid species, yet the mechanisms underlying the formation of amyloid polymorphs and their complex architectures remain elusive. Commonly, two main mesoscopic topologies are found in amyloid polymorphs cha ...

Wiley2024

Effect of antibiotics on mechanical properties of Bordetella pertussis examined by atomic force microscopy

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Leonardo Venturelli

In recent years, the coevolution of microorganisms with current antibiotics has increased the mechanisms of bacterial resistance, generating a major health problem worldwide. Bordetella pertussis is a bacterium that causes whooping cough and is capable of ...

PERGAMON-ELSEVIER SCIENCE LTD2022

Environmental Control of Amyloid Polymorphism by Modulation of Hydrodynamic Stress

Giovanni Dietler, Sandor Kasas, Serguei Sekatski, François Gallaire, Jiangtao Zhou, Leonardo Venturelli, Ludovic Pierre Gustave Keiser

The phenomenon of amyloid polymorphism is a key feature of protein aggregation. Unravelling this phenomenon is of great significance for understanding the underlying molecular mechanisms associated with neurodegenerative diseases and for the development of ...

AMER CHEMICAL SOC2021

The Nt17 Domain and its Helical Conformation Regulate the Aggregation, Cellular Properties and Neurotoxicity of Mutant Huntingtin Exon 1

Giovanni Dietler, Hilal Lashuel, Anass Chiki, Francesco Simone Ruggeri, Anne-Laure Mahul Mellier, Sophie Vieweg, Sean Michael Deguire, Urszula Beata Cendrowska, Nathan Alain Denis Riguet

Converging evidence points to the N-terminal domain comprising the first 17 amino acids of the Huntingtin protein (Nt17) as a key regulator of its aggregation, cellular properties and toxicity. In this study, we further investigated the interplay between N ...

Elsevier2021

Nanomechanical bio-sensing for fast and reliable detection of viability and susceptibility of microorganisms

Giovanni Dietler, Raphaël Foschia, Sandor Kasas, María Inés Villalba, Leonardo Venturelli

The metabolic activity of living organisms results in cellular motion on a nanometric scale that can be efficiently detected by micro- and nano- fabricated sensors. Quartz crystal microbalance and atomic force microscopy (AFM) inspired techniques have rece ...

2021

Nanomotion Spectroscopy as a New Approach to Characterize Bacterial Virulence

Giovanni Dietler, Sandor Kasas, Giovanni Longo, María Inés Villalba, Leonardo Venturelli

Atomic force microscopy (AFM)-based nanomotion detection is a label-free technique that has been used to monitor the response of microorganisms to antibiotics in a time frame of minutes. The method consists of attaching living organisms onto an AFM cantile ...

MDPI2021

Structural and DNA binding properties of mycobacterial integration host factor mIHF

Giovanni Dietler, Stewart Cole, Aleksandre Japaridze, Rajkumar Singh, Luciano Andres Abriata, Nina Theres Odermatt, Jérémie Piton, David Lyndon Emsley, Moreno Lelli

In bacteria, nucleoid associated proteins (NAPs) take part in active chromosome organization by supercoil management, three-dimensional DNA looping and direct transcriptional control. Mycobacterial integration host factor (mIHF, rv1388) is a NAP restricted ...

Elsevier2020

Effects of sedimentation, microgravity, hydrodynamic mixing and air-water interface on alpha-synuclein amyloid formation

Giovanni Dietler, Giovanni Longo, Francesco Simone Ruggeri, Jiangtao Zhou

The formation of amyloid fibrils is a characterizing feature of a range of protein misfolding diseases, including Parkinson's disease. The propensity of native proteins to form such amyloid fibril, both in vitro and in vivo, is highly sensitive to the surr ...

2020

Yeast Nanometric Scale Oscillations Highlights Fibronectin Induced Changes in C. albicans

Giovanni Dietler, Sandor Kasas, Anne-Céline Verenna Kohler, Leonardo Venturelli

Yeast resistance to antifungal drugs is a major public health issue. Fungal adhesion onto the host mucosal surface is still a partially unknown phenomenon that is modulated by several actors among which fibronectin plays an important role. Targeting the ye ...

2020

A perspective view on the nanomotion detection of living organisms and its features

Giovanni Dietler, Sandor Kasas, Anne-Céline Verenna Kohler, Giovanni Longo, Massimiliano Bertacchi, Petar Stupar, María Inés Villalba, Leonardo Venturelli

The insurgence of newly arising, rapidly developing health threats, such as drug-resistant bacteria and cancers, is one of the most urgent public-health issues of modern times. This menace calls for the development of sensitive and reliable diagnostic tool ...

WILEY2020

Electrical Excitation of Long-Range Surface Plasmons in PC/OLED Structure with Two Metal Nanolayers

Giovanni Dietler, Serguei Sekatski

A current-driven source of long-range surface plasmons (LRSPs) on a duplex metal nanolayer is reported. Electrical excitation of LRSPs was experimentally observed in a planar structure, where an organic light-emitting film was sandwiched between two metal ...

SHANGHAI JIAO TONG UNIV PRESS2020

Adaptation of Pseudomonas aeruginosa to constant sub-inhibitory concentrations of quaternary ammonium compounds

Florian Frédéric Vincent Breider, Giovanni Dietler, Sandor Kasas, Myriam Borgatta, Margaux Océane Voumard, Urs von Gunten, Leonardo Venturelli

Quaternary ammonium compounds (QACs) are widely used in consumer products for disinfection purposes. QACs are frequently detected in aquatic systems at sub-inhibitory concentrations and were found to affect the development of antimicrobial resistance if ba ...

2020

Single yeast cell nanomotions correlate with cellular activity

Giovanni Dietler, Sandor Kasas, María Inés Villalba, Anton Malovichko

Living single yeast cells show a specific cellular motion at the nanometer scale with a magnitude that is proportional to the cellular activity of the cell. We characterized this cellular nanomotion pattern of nonattached single yeast cells using classical ...

AMER ASSOC ADVANCEMENT SCIENCE2020

Gap Plasmon Enhanced High Spatial Resolution Imaging by Photothermal Induced Resonance in Visible Spectral Range

Giovanni Dietler, Serguei Sekatski, Anton Smirnov, Jiangtao Zhou

CELL PRESS2020

The role of xanthophylls in the supramolecular organization of the photosynthetic complex LHCII in lipid membranes studied by high-resolution imaging and nanospectroscopy

Giovanni Dietler, Serguei Sekatski, Jiangtao Zhou

The xanthophyll cycle is a regulatory mechanism operating in the photosynthetic apparatus of plants. It consists of the conversion of the xanthophyll pigment violaxanthin to zeaxanthin, and vice versa, in response to light intensity. According to the curre ...

ELSEVIER2020

Fiber-Tip Polymer Microcantilever for Fast and Highly Sensitive Hydrogen Measurement

Giovanni Dietler, Sandor Kasas, Meng Zhu, Cong Xiong, Jiangtao Zhou, Leonardo Venturelli, Chaohui Li, Yiping Wang

Hydrogen as an antioxidant gas has been widely used in the medical and biological fields for preventing cancer or treating inflammation. However, controlling the hydrogen concentration is crucial for practical use due to its explosive property when its vol ...

AMER CHEMICAL SOC2020

Gap-Plasmon-Enhanced High-Spatial-Resolution Imaging by Photothermal-Induced Resonance in the Visible Range

Giovanni Dietler, Serguei Sekatski, Anton Smirnov, Jiangtao Zhou

Chemical characterization at the nanoscale is of significant importance for many applications in physics, analytical chemistry, material science, and biology. Despite the intensive studies in the infrared range, high-spatial resolution and high-sensitivity ...

AMER CHEMICAL SOC2019

Experimenting with knots

Giovanni Dietler

Knots are interesting objects to experiment since their topological properties are combined with mechanical properties, fluid mechanics or dynamical properties like unknotting under the excitation of the thermal energy. Knots play also a role in biology be ...

SPRINGER HEIDELBERG2019

Chemically etched plastic optical fiber probe for near-field scanning optical microscopy in liquids

Giovanni Dietler, Serguei Sekatski, Kanat Dukenbayev, Anton Smirnov

Some preliminary results and experimental details of Near-field Scanning Optical Microscopy (NSOM) operation in liquids have been reported by us earlier(1-4). Here we present the first use of custom made polystyrene/poly (methyl methacrylate) (PMMA) optica ...

SPIE-INT SOC OPTICAL ENGINEERING2019