Publications de Joachim Piguet

Single-Molecule Imaging Deciphers the Relation between Mobility and Signaling of a Prototypical G Protein-Coupled Receptor in Living Cells

Lateral diffusion enables efficient interactions between membrane proteins leading to signal transmission across the plasma membrane. An open question is how the spatio-temporal distribution of cell surface receptors influences the transmembrane signaling ...

American Society for Biochemistry and Molecular Biology2015

Single-Molecule Microscopy Deciphers the Relation between Trafficking and Signaling of the NK1 Receptor in Livings Cells

Horst Vogel, Joachim Piguet, Luc Veya

Cell Press2014

Tracking NK1 receptor diffusion in the membrane of living cells. Effect of receptor activation

Horst Vogel, Joachim Piguet, Luc Veya

Springer2013

Downscaling the Analysis of Complex Transmembrane Signaling Cascades to Closed Attoliter Volumes

Rudolf Hovius, Horst Vogel, Joachim Piguet, Luigino Grasso, Romain Wyss, Michael Werner, Ghérici Hassaïne

Cellular signaling is classically investigated by measuring optical or electrical properties of single or populations of living cells. Here we show that ligand binding to cell surface receptors and subsequent activation of signaling cascades can be monitor ...

Public Library of Science2013

Tracking NK1 receptor diffusion in the membrane of living cells. Roles of clathrin and cytoskeleton

Horst Vogel, Joachim Piguet, Luc Veya

Springer2013

Microfluidic array cytometer based on refractive optical tweezers for parallel trapping, imaging and sorting of individual cells

René Salathé, Horst Vogel, Fabrice Merenda, Joachim Piguet, Michael Werner

Analysis of genetic and functional variability in populations of living cells requires experimental techniques capable of monitoring cellular processes such as cell signaling of many single cells in parallel while offering the possibility to sort interesti ...

Royal Society of Chemistry2011

Acetylcholine Receptor Organization in Membrane Domains in Muscle Cells: EVIDENCE FOR RAPSYN-INDEPENDENT AND RAPSYN-DEPENDENT MECHANISMS

Rudolf Hovius, Horst Vogel, Jean-Manuel Segura, Joachim Piguet, Christoph Schreiter

Nicotinic acetylcholine receptors (nAChR) in muscle fibers are densely packed in the postsynaptic region at the neuromuscular junction. Rapsyn plays a central role in directing and clustering nAChR during cellular differentiation and neuromuscular junction ...

American Society for Biochemistry and Molecular Biology2011

Semisynthesis of fluorescent metabolite sensors on cell surfaces

Horst Vogel, Kai Johnsson, Joachim Piguet, Luc Reymond, Matthias Albert Brun, Kui Thong Tan, Ruud Peters, Rudolf Griss

Progress in understanding signal transduction and metabolic pathways is hampered by a shortage of suitable sensors for tracking metabolites, second messengers, and neurotransmitters in living cells. Here we introduce a class of rationally designed semisynt ...

2011

Advanced Fluorescence Microscopy to Study Plasma Membrane Protein Dynamics

Joachim Piguet

Membrane protein dynamics is of great importance for living organisms. The precise localization of proteins composing a synapse on the membrane facing a nerve terminus is essential for proper functioning of the nervous system. In muscle fibers, the nicotin ...

EPFL2010

Monitoring the Diffusion of Single Heterotrimeric G Proteins in Supported Cell-membrane Sheets Reveals their Partitioning into Microdomains

Jean-Baptiste Perez, Horst Vogel, Jean-Manuel Segura, Joachim Piguet

Supported cell-membrane sheets are promising in vitro systems to investigate the properties of membranes with native protein/lipid composition, in particular their sub-compartmentalization and the differential localization of proteins associated to them. W ...

Elsevier2006