Publications de Sophie Nallet | EPFL Graph Search

Glycan variability on a recombinant IgG antibody transiently produced in HEK-293E cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, Simone Schmitt, Yury Tsybin, Luca Fornelli, Julien Parra

In this study, a recombinant monoclonal IgG antibody was produced by transient gene expression (TGE) in suspension-adapted HEK-293E cells. The objective of the study was to determine the variation in recombinant IgG yield and glycosylation in ten independe ...

Elsevier2012

Poly(ethyleneimine)-Mediated Large-Scale Transient Gene Expression: Influence of Molecular Weight, Polydispersity and N-Propionyl Groups

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker, Harm-Anton Klok, Zuzana Kadlecova

Three synthesis lots of linear poly(ethyleneimine) (PEI) are compared to a fully hydrolyzed linear PEI (commercially available as PEI Max) regarding structure, polyplex formation with plasmid DNA, and transfection of suspension-adapted HEK-293E cells. PEI ...

Wiley-Blackwell2012

Structural analysis of intact monoclonal antibodies by electron transfer dissociation mass spectrometry

Florian Maria Wurm, Sophie Nallet, Yury Tsybin, Luca Fornelli, Julien Parra

Improving qualitative and quantitative characterization of monoclonal antibodies is essential, because of their increasing popularity as therapeutic drug targets. Electron transfer dissociation (ETD)-based top-down mass spectrometry (MS) is the method of c ...

2011

Transient Gene Expression in HEK-293E Cells for Recombinant Protein Production : Process Characterization towards GMP Approval

Sophie Nallet

The growing demand of biopharmaceutical products is boosting the market for therapeutic recombinant proteins (r-proteins). More than half of the 140 r-proteins that have gained approval for human therapeutic use are manufactured in mammalian cells that mak ...

EPFL2010

Respiratory syncytial virus subunit vaccine based on a recombinant fusion protein expressed transiently in mammalian cells

Florian Maria Wurm, Lucia Baldi Unser, Sophie Nallet, David Hacker

Although respiratory syncytial virus (RSV) causes severe lower respiratory tract infection in infants and adults at risk, no RSV vaccine is currently available. In this report, efforts toward the generation of an RSV subunit vaccine using recombinant RSV f ...

2009