Publications de Sébastien Schaub

Improving 3D MA-TIRF Reconstruction with Deconvolution and Background Estimation

Sébastien Schaub, Emmanuel Emilien Louis Soubies

Total internal reflection fluorescence microscopy (TIRF) produces 2D images of the fluorescent activity integrated over a very thin layer adjacent to the glass coverslip. By varying the illumination angle (multi-angle TIRF), a stack of 2D images is acquire ...

IEEE2019

Nanometric axial resolution of fibronectin assembly units achieved with an efficient reconstruction approach for multi-angle-TIRF microscopy

Sébastien Schaub, Emmanuel Emilien Louis Soubies

High resolution imaging of molecules at the cell-substrate interface is required for understanding key biological processes. Here we propose a complete pipeline for multi-angle total internal reflection fluorescence microscopy (MA-TIRF) going from instrume ...

Nature Research2019

Comparative dynamics of retrograde actin flow and focal adhesions: formation of nascent adhesions triggers transition from fast to slow flow

Jean-Jacques Meister, Alexander Verkhovskiy, Sébastien Schaub

Dynamic actin network at the leading edge of the cell is linked to the extracellular matrix through focal adhesions (FAs), and at the same time it undergoes retrograde flow with different dynamics in two distinct zones: the lamellipodium (peripheral zone o ...

Public Library of Science2008

Myofibroblast communication is controlled by intercellular mechanical coupling

Jean-Jacques Meister, Lysianne Follonier Castella, Sébastien Schaub, Boris Hinz

Neoformation of intercellular adherens junctions accompanies the differentiation of fibroblasts into contractile myofibroblasts, a key event during development of fibrosis and in wound healing. We have previously shown that intercellular mechanical couplin ...

Company of Biologists2008

Comparative maps of motion and assembly of filamentous actin and myosin II in migrating cells

Jean-Jacques Meister, Sophie Bohnet, Alexander Verkhovskiy, Sébastien Schaub

To understand the mechanism of cell migration, one needs to know how the parts of the motile machinery of the cell are assembled and how they move with respect to each other. Actin and myosin II are thought to be the major structural and force-generating c ...

American Society for Cell Biology2007

Analysis of actin filament network organization in lamellipodia by comparing experimental and simulated images

Jean-Jacques Meister, Alexander Verkhovskiy, Sébastien Schaub

Protrusion of lamellipodia during cell migration depends on the assembly of actin network. The assembly mechanism, based on dendritic filament branching, has been investigated in reconstituted in vitro systems, but little is known about the dynamical and s ...

Company of Biologists2007

Structure and dynamics of actin network at the leading edge of migrating cells

Sébastien Schaub

Crawling motion is characteristic of most animal cells and is principally based on actin-myosin II cytoskeletal system. The major components and reactions contributing to motility have been identified, but the overall picture of how these molecular events ...

EPFL2006