Publications de Claudio Dellagiacoma

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Marcel Leutenegger, Theo Lasser, Claudio Dellagiacoma, Corinne Berclaz, Noelia Laura Bocchio, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

Cumulant microscopy

Theo Lasser, Claudio Dellagiacoma, Stefan Geissbuehler

The invention describes a method and a microscopy system for imaging and analysing stochastically and independently blinking point-like emitters. A multiple-order cumulants analysis in conjunction with an established blinking model enables the extraction o ...

2012

Comparison between SOFI and STORM

Theo Lasser, Claudio Dellagiacoma, Stefan Geissbühler

A straightforward method to achieve super-resolution consists of taking an image sequence of stochastically blinking emitters using a standard wide-field fluorescence microscope. Densely packed single molecules can be distinguished sequentially in time usi ...

2011

Single Molecule Sensing

Claudio Dellagiacoma

Living organisms consist of cells, the elementary components of which are proteins providing cellular structures and functionality. Nowadays, proteins of the size of one to a few tens of nanometers can be efficiently monitored by fluorescence microscopy an ...

EPFL2011

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Iwan Märki, Theo Lasser, Kai Johnsson, Claudio Dellagiacoma, Stefan Geissbühler, Sambashiva Banala, Grazvydas Lukinavicius, Noelia Laura Bocchio

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010

Direct Observation of Transitions between Surface-Dominated and Bulk Diffusion Regimes in Nanochannels

Philippe Renaud, Arnaud Bertsch, Theo Lasser, Nicolas Durand, Claudio Dellagiacoma, Raphaël Goetschmann

The diffusion of charged proteins in liquid-filled nanometer-sized apertures with charged surfaces has been investigated with fluorescence correlation spectroscopy (FCS). Based on a two-dimensional (2D) multicomponent diffusion model, key parameters such a ...

2009

Experimental comparison between conventional and hybrid long-range surface plasmon waveguide bends

Claudio Dellagiacoma, Olivier Martin

We report on the characterization of long-range surface plasmon waveguide bends at telecom wavelengths (lambda=1550 nm). The structures consist of a thin Au stripe embedded in a transparent polymer film. When the polymer thickness is larger than the latera ...

2008

From metamaterials to plasmonics: the advancing role of metals in optics

Claudio Dellagiacoma, Olivier Martin

2007