Publications de Stéphanie Tissot

Personalized cancer vaccine strategy elicits polyfunctional T cells and demonstrates clinical benefits in ovarian cancer

Stéphanie Tissot, Johanna Pauline Chiffelle, George Coukos, Alexandre Harari, Florian Huber

T cells are important for controlling ovarian cancer (OC). We previously demonstrated that combinatorial use of a personalized whole-tumor lysate-pulsed dendritic cell vaccine (OCDC), bevacizumab (Bev), and cyclophosphamide (Cy) elicited neoantigen-specifi ...

2021

Numerical simulation of orbitally shaken viscous fluids with free surface

Florian Maria Wurm, Alfio Quarteroni, Marco Discacciati, David Hacker, Stéphanie Tissot, Samuel Quinodoz

Orbitally shaken bioreactors are an emerging alternative to stirred-tank bioreactors for large-scale mammalian cell culture, but their fluid dynamics is still not well defined. Among the theoretical and practical issues that remain to be resolved, the char ...

Wiley-Blackwell2013

Helical-Track Bioreactors for Bacterial, Mammalian and Insect Cell Cultures

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Stéphanie Tissot, Clara Joséphine Douet

We investigated the cultivation of bacterial, mammalian and insect cells in an orbitally-shaken 250-mL disposable tube that incorporates a helical track (HT) on its inside wall. The mass transfer coefﬁcient of oxygen (kLa) was 200%–400% higher in the HT tu ...

2013

k(L)a as a predictor for successful probe-independent mammalian cell bioprocesses in orbitally shaken bioreactors

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Stéphanie Tissot

The aim of this study was to gain a better understanding of orbitally shaken bioreactors (OSRs) operated without controllers for pH and dissolved oxygen (DO) concentration. We used cylindrical OSRs with working volumes ranging from 250mL to 200L to determi ...

Elsevier2012

OrbShake Bioreactors for Mammalian Cell Cultures : Engineering and Scale-up

Stéphanie Tissot

Most of the biopharmaceuticals nowadays are produced by animal cells. The worldwide demand for a specific group of biopharmaceuticals, the monoclonal antibodies (mAbs), has significantly increased this last decade. The mAbs are used as therapeutics in part ...

EPFL2011

Efficient and reproducible mammalian cell bioprocesses without probes and controllers?

Florian Maria Wurm, Lucia Baldi Unser, Mohamed Farhat, David Hacker, Stéphanie Tissot, Martino Reclari, Matthieu Dreyer, Agata Oberbek

Bioprocesses for recombinant protein production with mammalian cells are typically controlled for several physicochemical parameters including the pH and dissolved oxygen concentration (DO) of the culture medium. Here we studied whether these controls are ...

Elsevier2011

Hydrodynamic stress in orbitally shaken bioreactors

Florian Maria Wurm, Lucia Baldi Unser, Alfio Quarteroni, Mohamed Farhat, Marco Discacciati, David Hacker, Stéphanie Tissot, Martino Reclari, Samuel Quinodoz, Matthieu Dreyer, Dominique Terry Monteil Jr

2011

Determination of a scale-up factor from mixing time studies in orbitally shaken bioreactors

Christos Comninellis, Florian Maria Wurm, Mohamed Farhat, David Hacker, Stéphanie Tissot

Efficient mixing in bioreactors is essential in order to avoid concentration gradients which can be harmful for mammalian cells. To study mixing and its scalability in orbitally shaken cylindrical bioreactors, we measured mixing times in containers with no ...

Elsevier2010

Innovative, Non-stirred Bioreactors in Scales from Milliliters up to 1000 Liters for Suspension Cultures of Cells using Disposable Bags and Containers – A Swiss Contribution

Florian Maria Wurm, Lucia Baldi Unser, Mathieu Benjamin Stettler, David Hacker, Stéphanie Tissot, Gilles Broccard, Xiaowei Zhang

Innovative mixing principles in bioreactors, for example using the rocking of a platform to induce a backwards and forwards 'wave' , or using orbital shaking to generate a 'wave' that runs round in a cylindrical container, have proved to be successful for ...

Schweizerische Chemische Gesellschaft2010

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, Sophie Bouchet, David Hacker, Stéphanie Tissot, Jean Cevey, Sarah Wulhfard

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008