Publications de Thomas Lemmin | EPFL Graph Search

Effect of the Synaptic Plasma Membrane on the Stability of the Amyloid Precursor Protein Homodimer

Thomas Lemmin, Matteo Dal Peraro, Alessandro Barducci, Martina Audagnotto

The proteolytic cleavage of the transmembrane (TM) domain of the amyloid precursor protein (APP) releases amyloid-beta (A beta) peptides, which accumulation in the brain tissue is an early indicator of Alzheimer's disease. We used multiscale molecular dyna ...

Amer Chemical Soc2016

Lipid Builder: A Framework To Build Realistic Models for Biological Membranes

Thomas Lemmin, Matteo Dal Peraro, Christophe Bovigny, Nicolas Grégoire Maïno, Giorgio Elikem Tamo

The physical and chemical characterization of biological membranes is of fundamental importance for understanding the functional role of lipid bilayers in shaping cells and organelles, steering vesicle trafficking and promoting membrane-protein signaling. ...

Amer Chemical Soc2015

A coiled coil switch mediates cold sensing by the thermosensory protein DesK

Thomas Lemmin, Matteo Dal Peraro, Luciano Andres Abriata

The thermosensor histidine kinase DesK from Bacillus subtilis senses changes in membrane fluidity initiating an adaptive response. Structural changes in DesK have been implicated in transmembrane signaling, but direct evidence is still lacking. On the basi ...

Wiley-Blackwell2015

Perturbations of the Straight Transmembrane alpha-Helical Structure of the Amyloid Precursor Protein Affect Its Processing by gamma-Secretase

Thomas Lemmin, Patrick Fraering, Matteo Dal Peraro, Mitko Dimitrov

Background: Amyloid- neurotoxicity depends on the specificity of the proteolytic cleavage of the amyloid precursor protein (APP) transmembrane domain. Results: The APP transmembrane -helix is straight in a biological membrane bilayer. Conclusion: The flexi ...

American Society for Biochemistry and Molecular Biology2014

Reaction Mechanism and Catalytic Fingerprint of Allantoin Racemase

Matteo Thomas Degiacomi, Thomas Lemmin, Matteo Dal Peraro, Marco Stenta, Christophe Bovigny

The stereospecific oxidative decomposition of urate into allantoin is the core of purine catabolism in many organisms. The spontaneous decomposition of upstream intermediates and the nonenzymatic racemization of allantoin lead to an accumulation of (R)-all ...

Amer Chemical Soc2014

Critical roles of isoleucine-364 and adjacent residues in a hydrophobic gate control of phospholipid transport by the mammalian P4-ATPase ATP8A2

Thomas Lemmin, Matteo Dal Peraro, Anna Vestergaard

P4-ATPases (flippases) translocate specific phospholipids such as phosphatidylserine from the exoplasmic leaflet of the cell membrane to the cytosolic leaflet, upholding an essential membrane asymmetry. The mechanism of flipping this giant substrate has re ...

National Academy of Sciences2014

The Amyloid Precursor Protein Maintains an Ideal&Alpha-Helical Conformation in the Lipid Bilayer

Thomas Lemmin, Patrick Fraering, Matteo Dal Peraro, Mitko Dimitrov

Cell Press2014

Alzheimer's disease mutations in APP but not γ-secretase modulators affect epsilon-cleavage-dependent AICD production

Thomas Lemmin, Patrick Fraering, Freddy Radtke, Rajwinder Lehal, Jemila Houacine, Matteo Dal Peraro, Mitko Dimitrov, Andrzej Jacek Fligier

Pathological amino-acid substitutions in the amyloid precursor protein (APP) and chemical gamma-secretase modulators affect the processing of APP by the gamma-secretase complex and the production of the amyloid-beta peptide A beta 42, the accumulation of w ...

Nature Publishing Group2013

Molecular Modeling of Membrane Embedded Proteins

Thomas Lemmin

Over the past years, molecular modeling and simulation techniques have had a major impact on experimental life sciences. They are capable of providing accurate insight into microscopic mechanisms, which are usually difficult to investigate experimentally. ...

EPFL2013

Intrinsic Transmembrane Flexibility of the Amyloid Precursor Protein Explored by Molecular Simulations

Thomas Lemmin, Patrick Fraering, Matteo Dal Peraro, Mitko Dimitrov

Cell Press2013

In-depth analysis of hyaline fibromatosis syndrome frameshift mutations at the same site reveal the necessity of personalized therapy

Thomas Lemmin, Françoise Gisou van der Goot Grunberg, Suzanne Salvi, Matteo Dal Peraro, Shixu Yan

Hyaline fibromatosis syndrome is an autosomal recessive disease caused by mutations in ANTXR2, a gene involved in extracellular matrix homeostasis. Sixty percent of patients carry frameshift mutations at a mutational hotspot in exon 13. We show in patient ...

Wiley-Blackwell2013

Assembly of the Transmembrane Domain of E. coli PhoQ Histidine Kinase: Implications for Signal Transduction from Molecular Simulations

Thomas Lemmin, Matteo Dal Peraro

The PhoQP two-component system is a signaling complex essential for bacterial virulence and cationic antimicrobial peptide resistance. PhoQ is the histidine kinase chemoreceptor of this tandem machine and assembles in a homodimer conformation spanning the ...

Public Library Science2013

Cardiolipin Models for Molecular Simulations of Bacterial and Mitochondrial Membranes

Thomas Lemmin, Diane Virginie Marie Lançon, Matteo Dal Peraro, Christophe Bovigny

Present in bacterial and mitochondrial membranes, cardiolipins have a unique dimeric structure, which carries up to two charges (i.e., one per phosphate group) and, under physiological conditions, can be unprotonated or singly protonated. Exhaustive models ...

Amer Chemical Soc2013

Palmitoylated calnexin is a key component of the ribosome-translocon complex

Thomas Lemmin, Béatrice Kunz, Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami, Matteo Dal Peraro, Sanja Blaskovic

A third of the human genome encodes N-glycosylated proteins. These are co-translationally translocated into the lumen/membrane of the endoplasmic reticulum (ER) where they fold and assemble before they are transported to their final destination. Here, we s ...

2012

Model-based attenuation of movement artifacts in fMRI

Thomas Lemmin

Behavioral analysis of multi-joint arm reaching has allowed important advances in understanding the control of voluntary movements. Complementing this analysis with functional magnetic resonance imaging (fMRI) would give insight into the neural mechanisms ...

2010