Publications de Frédéric Lacharme

Microfluidic applications of magnetic particles for biological analysis and catalysis

Martinus Gijs, Frédéric Lacharme, Ulrike Lehmann

American Chemical Society2010

Sandwich immunoassay on a microfluidic chip using patterns of electrostatically self-assembled streptavidin-coated beads

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme, Venkataragavalu Sivagnanam

In this paper, we demonstrate a bead-based microfluidic immunoassay using self-assembled streptavidin-coated bead micropatterns. The beads are assembled on the microfluidic channel surface using electrostatic forces between the negatively-charged streptavi ...

Elsevier2009

Magnetic beads retention device for sandwich immunoassay: comparison of off-chip and on-chip antibody incubation

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme

We use magnetic microbeads, which are magnetically self-assembled in chains in a microfluidic chip, as reaction substrates to implement two different sandwich immunoassay protocols for the detection of mouse monoclonal target antibodies. The magnetic chain ...

Springer-Verlag2009

Full on-chip nanoliter immuno-assay by geometrical magnetic trapping of nanoparticle chains

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme

We propose an original concept to perform a complete on-chip sandwich immunoassay on magnetic nanoparticles that are self-assembled in chains in a uniform magnetic field. The magnetic chains are retained over periodically enlarged cross sections of a micro ...

2008

Separation, detection and quantification of biomolecules in lab-on-a-chip systems

Frédéric Lacharme

Since the introduction of the of Micro Total Analysis Systems concept in 1990, important progress in microfluidic analytical systems has been realized. In many cases, microfluidic systems have shown improved analytical performances over classical methods, ...

EPFL2008

Magnetic bead retention device for full on-chip sandwich immuno-assay

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme

2008

On-chip nanoliter immunoassay by geometrical trapping of magnetic nanoparticle chains

Martinus Gijs, Caroline Vandevyver, Frédéric Lacharme

2008

Magnetic bead retention apparatus and method

Martinus Gijs, Frédéric Lacharme

An apparatus for retaining magnetic particles in self-assembled magnetic particle structures in a liquid flow, comprises a micro-channel (5) and magnets (1,2) for generating a substantially static magnetic field across the micro-channel (5) such that magne ...

2007

Pressure injection in continuous sample flow electrophoresis microchips

Martinus Gijs, Frédéric Lacharme

Pressure pulse injection is proposed as an alternative technique to electrokinetic injection for use in continuous sample flow electrophoresis microchips. During chip operation, sample and buffer solutions are electrokinetically driven near to the entrance ...

2006

Single potential electrophoresis microchip with reduced bias using pressure pulse injection

Martinus Gijs, Frédéric Lacharme

2006

Pumping of mammalian cells with a nozzle-diffuser micropump

Christophe Yamahata, Horst Vogel, Martinus Gijs, Caroline Vandevyver, Paulina Izewska, Frédéric Lacharme

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membran ...

Royal Society of Chemistry2005

Glass valveless micropump using electromagnetic actuation

Christophe Yamahata, Martinus Gijs, Frédéric Lacharme

We present a valveless micropump in glass, which is magnetically actuated using the sinusoidal current of an external electromagnet. We employ a powder blasting microerosion process for microstructuring the glass substrates and fusion bonding for assembly ...

2005

Electromagnetically Actuated Ball Valve Micropumps

Christophe Yamahata, Martinus Gijs, Frédéric Lacharme

We present two types of oscillating diaphragm micropumps configured with passive ball valves and using electromagnetic actuation. One type is made out of poly(methyl methacrylate) (PMMA), while the other one is made out of borosilicate glass. Both were pro ...

2005

A ball valve micropump in glass fabricated by powder blasting

Christophe Yamahata, Martinus Gijs, Frédéric Lacharme

We present the microfabrication and characterisation of a ball valve micropump in glass, which is magnetically actuated using the sinusoidal current of an external electromagnet. We employ the use of a simple powder blasting technology for microstructuring ...

2005