Publications de Thomas Michael Braschler

Low temperature and mTOR inhibition favor human epidermal stem cell maintenance

Juan Ramon Mosig, Fahd Azzabi Zouraq, Kai Johnsson, Yann Barrandon, Ariane Rochat, Thomas Michael Braschler, Johannes Alexander Mosig, Jean-Baptiste Bureau

ELSEVIER SCIENCE INC2023

Highly Efficient Cardiac Differentiation and Maintenance by Thrombin-Coagulated Fibrin Hydrogels Enriched with Decellularized Porcine Heart Extracellular Matrix

Philippe Renaud, Thomas Michael Braschler, Fatemeh Navaee

Biochemical and biophysical properties instruct cardiac tissue morphogenesis. Here, we are reporting on a blend of cardiac decellularized extracellular matrix (dECM) from porcine ventricular tissue and fibrinogen that is suitable for investigations employi ...

MDPI2023

Low temperature and mTOR inhibition favor stem cell maintenance in human keratinocyte cultures

Fahd Azzabi Zouraq, Kai Johnsson, Yann Barrandon, Ariane Rochat, Thomas Michael Braschler, Johannes Alexander Mosig, Jean-Baptiste Bureau, Mako Kamiya

Adult autologous human epidermal stem cells can be extensively expanded ex vivo for cell and gene therapy. Identifying the mechanisms involved in stem cell maintenance and defining culture conditions to maintain stemness is critical, because an inadequate ...

WILEY2023

Device and process for tissue-engineering and regenerative medicine

Thomas Michael Braschler, Olaia Maria Naveiras Torres-Quiroga, Amélie Barbara Hildegarde Béduer, Josefine Catharina Pedersen Tratwal, Martina Genta, Daniel Naveed Tavakol

A process and device for direct in-vitro to in-vivo transfer of a living microcarrier culture by controlled dehydration into a viscoelastic material capable of cell-protection during delivery. One particular purpose of the present invention is that of prov ...

2021

Cryogel-based Injectable 3D Microcarrier Co-culture for Support of Hematopoietic Progenitor Niches

Thomas Michael Braschler, Olaia Maria Naveiras Torres-Quiroga, Josefine Catharina Pedersen Tratwal, Martina Genta, Daniel Naveed Tavakol

Although hematopoietic stem cell (HSC) transplantation can restore functional hematopoiesis upon immune or chemotherapy-induced bone marrow failure, complications often arise during recovery, leading to up to 25% transplant-related mortality in treated pat ...

WILEY2021

An injectable, scalable, 3D tissue-engineered model of bone marrow adipogenesis and hematopoiesis

Thomas Michael Braschler, Olaia Maria Naveiras Torres-Quiroga, Martina Genta

E M H SWISS MEDICAL PUBLISHERS LTD2020

Particle suitable for the manufacture of an implantable soft tissue engineering material

Philippe Renaud, Thomas Michael Braschler, Amélie Barbara Hildegarde Béduer

The particle (1) is suitable for the manufacture of an implantable soft tissue engineering material and comprises: a three-dimensionally warped and branched sheet (2) where (i) the three-dimensionally warped and branched sheet (2) is made from a biocompati ...

2019

Pore Size Manipulation in 3D Printed Cryogels Enables Selective Cell Seeding

Philippe Renaud, Arnaud Bertsch, Ariane Rochat, Thomas Michael Braschler, Ludovic Serex, Amélie Barbara Hildegarde Béduer, Aleksandra Filippova

Cryogels are macroporous materials that display remarkable properties, such as high pore interconnection, large surface to volume ratio, and high mechanical stability, making them good candidates for 3D cell culture. However, shaping cryogels remains chall ...

Wiley2018

Additive manufacturing of hierarchical injectable scaffolds for tissue engineering

Philippe Renaud, Thomas Michael Braschler, Niccolo Piacentini

We present a 3D-printing technology allowing free-form fabrication of centimetre-scale injectable structures for minimally invasive delivery. They result from the combination of 3D printing onto a cryogenic substrate and optimisation of carboxymethylcellul ...

2018

Cryogel 3d scaffolds and methods for producing thereof

Philippe Renaud, Thomas Michael Braschler, Amélie Barbara Hildegarde Béduer

A method of producing a cryogel-based multicompartment 3D scaffold is herein disclosed. The method comprises the steps of: a) providing a first frozen polymeric layer on a refrigerated support kept at subzero temperature; b) providing subsequent polymeric ...

2018

Microfluidic sensor and method for obtaining such a sensor

Philippe Renaud, Marc Lany, Thomas Michael Braschler, David Vincent Bonzon, Georges Muller, Luc Gaëtan Aeberli, Jonas Choppe, Niklas Van Neyghem

The invention describes a method for producing hybrid microelectronic/microfluidic sensors at industrial scale. The method is characterized in that it comprises the following steps for obtaining said microfluidic channel: a) a first lamination step of a dr ...

2018

Dielectrophoresis as a single cell characterization method for bacteria

Philippe Renaud, Thomas Michael Braschler, John McKinney, Neeraj Dhar, Ana Valero

Real-time, quantitative characterization of cells at single-cell resolution, particularly while maintain- ing their intrinsic properties and without affecting cellular processes, is of primary importance in modern biological assays. Dielectrophoresis is a ...

Iop Publishing Ltd2017

Shapeable scaffold material and uses thereof

Philippe Renaud, Thomas Michael Braschler, Giorgio Pietramaggiori, Amélie Barbara Hildegarde Béduer

The invention relates to a scaffold material comprising a plurality of particles of a highly porous polymeric material, characterized in that said scaffold material becomes a shapeable paste once hydrated. The specific features of the particle material imp ...

2017

Ophthalmic contact lens with a compressible affinity matrix

Philippe Renaud, Thomas Michael Braschler, Amélie Barbara Hildegarde Béduer

The invention relates to a contact lens for use in the treatment of ocular inflammatory pathologies. The contact lens comprises a soft porous material coupled, in certain embodiments, with detoxifying agents. Said material and/or agents contact and neutral ...

2016

Soft nanofluidics governing minority ion exclusion in charged hydrogels

Fabien Patrick Wildhaber, Thomas Michael Braschler, Songmei Wu, Sidi Ahmed Bencherif

We investigate ionic partition of negatively charged molecular probes into also negatively charged, covalently crosslinked alginate hydrogels. The aim is to delimit the domain of validity of the major nanoelectrostatic models, and in particular to assess t ...

Royal Society of Chemistry2015

Injectable cryogel-based whole-cell cancer vaccines

Thomas Michael Braschler, Sidi Ahmed Bencherif

A biomaterial-based vaccination system that uses minimal extracorporeal manipulation could provide in situ enhancement of dendritic cell (DC) numbers, a physical space where DCs interface with transplanted tumour cells, and an immunogenic context. Here we ...

Nature Publishing Group2015

A Compressible Scaffold for Minimally Invasive Delivery of Large Intact Neuronal Networks

Philippe Renaud, Thomas Michael Braschler, Patrick Fraering, Oliver Peric, Georg Fantner, Sébastien Mosser, Amélie Barbara Hildegarde Béduer, Sidi Ahmed Bencherif

Millimeter to centimeter-sized injectable neural scaffolds based on macroporous cryogels are presented. The polymer-scaffolds are made from alginate and carboxymethyl-cellulose by a novel simple one-pot cryosynthesis. They allow surgical sterility by means ...

Wiley-VCH Verlag2015

Advances in the design of macroporous polymer scaffolds for potential applications in dentistry

Philippe Renaud, Thomas Michael Braschler, Sidi Ahmed Bencherif

A paradigm shift is taking place in medicine and dentistry from using synthetic implants and tissue grafts to a tissue engineering approach that uses degradable porous three-dimensional (3D) material hydrogels integrated with cells and bioactive factors to ...

2013

Device for application of medium to human tissue or animal body such as eye, has carrier which is provided with micro-cavity for containing medium in direct contact with object on which medium is to be applied

Hubert van den Bergh, Philippe Renaud, Yann Barrandon, Thomas Michael Braschler, Julien Graber

NOVELTY - The device has a carrier (1) which is provided with micro-cavity (2) for containing medium in direct contact with the object on which the medium is to be applied. Other carrier is provided with ring to protect the medium from environment when the ...

2013

On-Chip Flow Cytometry

Philippe Renaud, Thomas Michael Braschler, Ana Valero

Springer2012