Publications de Nicolas Dénervaud

Two distinct promoter architectures centered on dynamic nucleosomes control ribosomal protein gene transcription

Felix Naef, Philippe Jacquet, Nicolas Dénervaud, Sebastian Maerkl, Jacques Rougemont, Marcel Geertz

In yeast, ribosome production is controlled transcriptionally by tight coregulation of the 138 ribosomal protein genes (RPGs). RPG promoters display limited sequence homology, and the molecular basis for their coregulation remains largely unknown. Here we ...

Cold Spring Harbor Laboratory Press2014

Mapping the fine structure of a eukaryotic promoter input-output function

Nicolas Dénervaud, Sebastian Maerkl, Arun Stephen Rajkumar

The precise tuning of gene expression levels is essential for the optimal performance of transcriptional regulatory networks. We created 209 variants of the Saccharomyces cerevisiae PHO5 promoter to quantify how different binding sites for the transcriptio ...

Nature Publishing Group2013

A chemostat array enables the spatio-temporal analysis of the yeast proteome

Felix Naef, Michaël Unser, Nicolas Dénervaud, Sebastian Maerkl, Ricard Delgado Gonzalo, Arun Stephen Rajkumar, Johannes Becker

Observing cellular responses to perturbations is central to generating and testing hypotheses in biology. We developed a massively parallel microchemostat array capable of growing and observing 1,152 yeast-GFP strains on the single-cell level with 20 min t ...

National Academy of Sciences2013

A microfluidic live-cell imaging platform to study large collections of microbial genotypes

Nicolas Dénervaud

EPFL2013

KRAB-Zinc Finger Proteins and KAP1 Can Mediate Long-Range Transcriptional Repression through Heterochromatin Spreading

Philipp Bucher, Nicolas Dénervaud, Didier Trono, Sylvain Meylan, Anna Claire Groner, Giovanna Ambrosini, Nadine Fournier

Krüppel-associated box domain-zinc finger proteins (KRAB-ZFPs) are tetrapod-specific transcriptional repressors encoded in the hundreds by the human genome. In order to explore their as yet ill-defined impact on gene expression, we developed an ectopic rep ...

Public Library of Science2010

Multi-Target Tracking Of Packed Yeast Cells

Michaël Unser, Nicolas Dénervaud, Sebastian Maerkl, Ricard Delgado Gonzalo

The tracking of cell populations in time-lapse microscopy images enables high-throughput spatiotemporal measurements of cell dynamics. In this paper, we present a new algorithm to simultaneously track many cells in crowded areas. The algorithm runs in real ...

Ieee Service Center, 445 Hoes Lane, Po Box 1331, Piscataway, Nj 08855-1331 Usa2010