Publications de Alexandre de Titta

Adjuvant-free immunization with infective filarial larvae as lymphatic homing antigen carriers

Melody Swartz, Alexandre de Titta, Witold Waldemar Kilarski, Sachiko Hirosue, Marcela Rincón-Restrepo, Esra Güç, David Scott Wilson

Controlled infection with intestinal nematodes has therapeutic potential for preventing the symptoms of allergic and autoimmune diseases. Here, we engineered larvae of the filarial nematode Litomosoides sigmodontis as a vaccine strategy to induce adaptive ...

Nature Research2020

Toll-like receptor 8 agonist nanoparticles mimic immunomodulating effects of the live BCG vaccine and enhance neonatal innate and adaptive immune responses

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Sachiko Hirosue, Daniel Kenneth Bonner

Background: Newborns display distinct immune responses, leaving them vulnerable to infections and impairing immunization. Targeting newborn dendritic cells (DCs), which integrate vaccine signals into adaptive immune responses, might enable development of a ...

2017

Fibronectin EDA and CpG synergize to enhance antigen-specific Th1 and cytotoxic responses

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Ziad Julier, Eleonora Simeoni, Alizée Jéléna Grimm

Subunit vaccines, employing purified protein antigens rather than intact pathogens, require the addition of adjuvants for enhanced immunogenicity with a correct balance between strong activation of the immune system and low toxicity. Here we show that the ...

Elsevier Sci Ltd2016

Nanoparticle conjugation enhances the immunomodulatory effects of intranasally delivered CpG in house dust mite-allergic mice

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Marie Ballester, Laura Jeanbart, Benjamin John Marsland

An emerging strategy in preventing and treating airway allergy consists of modulating the immune response induced against allergens in the lungs. CpG oligodeoxynucleotides have been investigated in airway allergy studies, but even if promising, efficacy re ...

Nature Publishing Group2015

The TLR4 Agonist Fibronectin Extra Domain A is Cryptic, Exposed by Elastase-2; use in a fibrin matrix cancer vaccine

Mikaël Martino, Jeffrey Alan Hubbell, Alexandre de Titta, Ziad Julier, Laura Jeanbart

Fibronectin (FN) is an extracellular matrix (ECM) protein including numerous fibronectin type III (FNIII) repeats with different functions. The alternatively spliced FN variant containing the extra domain A (FNIII EDA), located between FNIII 11 and FNIII 1 ...

Nature Publishing Group2015

Enhancing Efficacy of Anticancer Vaccines by Targeted Delivery to Tumor-Draining Lymph Nodes

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Marie Ballester, Patricia Corthésy Henrioud, Pedro Romero, Laura Jeanbart

The sentinel or tumor-draining lymph node (tdLN) serves as a metastatic niche for many solid tumors and is altered via tumor-derived factors that support tumor progression and metastasis. tdLNs are often removed surgically, and therapeutic vaccines against ...

Amer Assoc Cancer Research2014

Materials and protein engineering approaches for modulating immunity

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Marie Ballester, Stephane Kontos, Kristen Lorentz, Alizée Jéléna Grimm, Laura Jeanbart, David Scott Wilson, Kym Lorenz Brünggel

Amer Chemical Soc2014

Engineering adjuvants for prophylactic and therapeutic subunit vaccines

Alexandre de Titta

EPFL2014

Nanoparticle conjugation of CpG enhances adjuvancy for cellular immunity and memory recall at low dose

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Marie Ballester, Ziad Julier, Laura Jeanbart

In subunit vaccines, strong CD8(+) T-cell responses are desired, yet they are elusive at reasonable adjuvant doses. We show that targeting adjuvant to the lymph node (LN) via ultrasmall polymeric nanoparticles (NPs), which rapidly drain to the LN after int ...

National Academy of Sciences2013

Peripherally Administered Nanoparticles Target Monocytic Myeloid Cells, Secondary Lymphoid Organs and Tumors in Mice

Jeffrey Alan Hubbell, Melody Swartz, Alexandre de Titta, Stephane Kontos, Iraklis Kourtis, Sachiko Hirosue

Nanoparticles have been extensively developed for therapeutic and diagnostic applications. While the focus of nanoparticle trafficking in vivo has traditionally been on drug delivery and organ-level biodistribution and clearance, recent work in cancer biol ...

Public Library of Science2013

Nanoparticle conjugation and pulmonary delivery enhance the protective efficacy of Ag85B and CpG against tuberculosis

Jeffrey Alan Hubbell, Melody Swartz, Cyntia De Piano, Alexandre de Titta, Marie Ballester, Miriella Carmela Pasquier, Eleonora Simeoni, John McKinney, Neeraj Dhar

Vaccines that drive robust T-cell immunity against Mycobacterium tuberculosis (Mtb) are needed both for prophylactic and therapeutic purposes. We have recently developed a synthetic vaccine delivery platform with Pluronic-stabilized polypropylene sulfide n ...

2011

Binding of silver nanoparticles to bacterial proteins depends on surface modifications and inhibits enzymatic activity

Alexandre de Titta, Rizlan Bernier-Latmani, Jan Dobias, Nicholas Wigginton

2010

Mechanisms of protein adhesion to bare and carbonate-coated silver nanoparticles

Alexandre de Titta, Rizlan Bernier-Latmani, Nicholas Wigginton

2009