Publications de Pierre Jean Arnould Wipff

Dispositif et procédé pour l'évaluation de la contraction cellulaire

A method for assessing cell contraction includes adhering contractile cells to an oxidized and cellular adhesion activated surface of a biocompatible silicone elastomer film. The biocompatible silicone elastomer film allows the cells to contract and wrinkl ...

2017

Myofibroblasts work best under stress

Boris Hinz, Pierre Jean Arnould Wipff

Myofibroblasts are reparative connective tissue cells that contribute to the reconstruction of injured tissue by secreting new extracellular matrix and by exerting high contractile force. Deregulation of these activities results in tissue contracture and d ...

Elsevier2009

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

Hicham Majd, Jean-Jacques Meister, Boris Hinz, Pierre Jean Arnould Wipff, Lara Buscemi Estefanell

Strain devices with expandable polydimethylsiloxane (PDMS) culture membranes are frequently used to stretch cells in vitro, mimicking mechanically dynamic tissue environments. To immobilize cell-adhesive molecules to the otherwise non-adhesive PDMS substra ...

Elsevier2009

Mechanical orchestration of tissue remodeling

Pierre Jean Arnould Wipff

After organ injury, a complex sequence of events is triggered to heal the tissue and to form a scar. Myofibroblasts, specialized contractile and secretory fibroblasts, are one of the many cell types that participate in the remodeling process. Myofibroblast ...

EPFL2008

Integrins and the activation of latent transforming growth factor beta1 - an intimate relationship

Boris Hinz, Pierre Jean Arnould Wipff

Integrins are crucial for the ability of cells to sense mechanical perturbations and to transmit intracellular stress to their environment. We here review the more recently discovered role of integrins in activating the pleiotrophic cytokine transforming g ...

Elsevier2008

Lkb1 is required for TGFβ-mediated myofibroblast differentiation

Boris Hinz, Pierre Jean Arnould Wipff

Inactivating mutations of the tumor-suppressor kinase gene LKB1 underlie Peutz-Jeghers syndrome (PJS), which is characterized by gastrointestinal hamartomatous polyps with a prominent smooth-muscle and stromal component. Recently, it was noted that PJS-typ ...

Company of Biologists2008

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Boris Hinz, Pierre Jean Arnould Wipff, Lara Buscemi Estefanell

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008

Myofibroblast contraction activates latent TGF-beta1 from the extracellular matrix

Jean-Jacques Meister, Boris Hinz, Pierre Jean Arnould Wipff

The conjunctive presence of mechanical stress and active transforming growth factor beta1 (TGF-beta1) is essential to convert fibroblasts into contractile myofibroblasts, which cause tissue contractures in fibrotic diseases. Using cultured myofibroblasts a ...

Rockefeller University Press2007