Publications de Miriella Carmela Pasquier

Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation

Jeffrey Alan Hubbell, Olivier Burri, Miriella Carmela Pasquier, Andreas Wiederkehr, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Gabriele Galliverti, Chiara Cianciaruso

A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and livecell microscopy. Guided by an observation that dispersed isle ...

Springer Nature2017

Primary human and rat beta cells release the intracellular autoantigens GAD65, IA-2 and proinsulin in exosomes together with cytokine-induced enhancers of immunity

Jeffrey Alan Hubbell, Melody Swartz, Miriella Carmela Pasquier, Davide Demurtas, Sachiko Hirosue, Romain Hamelin, Steinunn Baekkeskov Hanahan, Michele De Palma, Edward Allen Phelps, Chiara Cianciaruso

The target autoantigens in several organ-specific autoimmune diseases, including type 1 diabetes (T1D), are intracellular membrane proteins, whose initial encounter with the immune system is poorly understood. Here we propose a new model for how these prot ...

American Diabetes Association2017

Aberrant Accumulation of the Diabetes Autoantigen GAD65 in Golgi Membranes in Conditions of ER Stress and Autoimmunity

Jeffrey Alan Hubbell, Miriella Carmela Pasquier, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Chiara Cianciaruso

Pancreatic islet β-cells are particularly susceptible to endoplasmic reticulum (ER) stress, which is implicated in β-cell dysfunction and loss during the pathogenesis of type 1 diabetes (T1D). The peripheral membrane protein GAD65 is an autoantigen in huma ...

Amer Diabetes Assoc2016

Compartmentalization of GABA synthesis by GAD67 differs between pancreatic beta cells and neurons

Miriella Carmela Pasquier, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Chiara Cianciaruso

The inhibitory neurotransmitter GABA is synthesized by the enzyme glutamic acid decarboxylase (GAD) in neurons and in pancreatic β-cells in islets of Langerhans where it functions as a paracrine and autocrine signaling molecule regulating the function of i ...

Public Library of Science2015

VEGFR-3 Neutralization Inhibits Ovarian Lymphangiogenesis, Follicle Maturation, and Murine Pregnancy

Jeffrey Alan Hubbell, Melody Swartz, Jong Eun Ihm, Joseph Rutkowski, Miriella Carmela Pasquier, Didier Trono, Seung Tae Lee, Witold Waldemar Kilarski, Vivianne Greenwood

Lymphatic vessels surround follicles within the ovary, but their roles in folliculogenesis and pregnancy, as well as the necessity of Lymphangiogenesis in follicle maturation and health, are undefined. We used systemic delivery of mF4-31C1, a specific anta ...

Elsevier Science Inc2013

Impaired Humoral Immunity and Tolerance in K14-VEGFR-3-Ig Mice That Lack Dermal Lymphatic Drainage

Melody Swartz, Joseph Rutkowski, Miriella Carmela Pasquier, Susan Thomas

Lymphatic vessels transport interstitial fluid, soluble Ag, and immune cells from peripheral tissues to lymph nodes (LNs), yet the contribution of peripheral lymphatic drainage to adaptive immunity remains poorly understood. We examined immune responses to ...

American Association of Immunologists ; Oxford University Press2012

Nanoparticle conjugation and pulmonary delivery enhance the protective efficacy of Ag85B and CpG against tuberculosis

Jeffrey Alan Hubbell, Melody Swartz, Cyntia De Piano, Alexandre de Titta, Marie Ballester, Miriella Carmela Pasquier, Eleonora Simeoni, John McKinney, Neeraj Dhar

Vaccines that drive robust T-cell immunity against Mycobacterium tuberculosis (Mtb) are needed both for prophylactic and therapeutic purposes. We have recently developed a synthetic vaccine delivery platform with Pluronic-stabilized polypropylene sulfide n ...

2011

Engineered fibrin-binding VEGF-C variant promotes lymphangiogenesis in vitro and in vivo in a dermal wound healing model

Jeffrey Alan Hubbell, Melody Swartz, Miriella Carmela Pasquier, Witold Waldemar Kilarski, Esra Güç

2009

Cooperative and redundant roles of VEGFR-2 and VEGFR-3 signaling in adult lymphangiogenesis

Melody Swartz, Joseph Rutkowski, Miriella Carmela Pasquier

Activation of vascular endothelial growth factor ( VEGF) receptor-3 (VEGFR-3) by VEGF-C initiates lymphangiogenesis by promoting lymphatic proliferation and migration. However, it is unclear whether VEGFR-3 signaling is required beyond these initial stages ...

Federation of American Society of Experimental Biology2007