Publications de Aurélien Godinat

Portable bioluminescent platform for in vivo monitoring of biological processes in non-transgenic animals

Aurélien Godinat, Elena Goun, Arkadiy Bazhin, Ghyslain Martial Alain Budin, Pavlo Khodakivskyi, Aleksey Yevtodiyenko, Tamara Maric

Bioluminescent imaging (BLI) is one of the most powerful and widely used preclinical imaging modalities. However, the current technology relies on the use of transgenic luciferase-expressing cells and animals and therefore can only be applied to a limited ...

Nature Research2021

Portable bioluminescence systems and methods for in vivo monitoring of molecular and metabolic events in animals

Aurélien Godinat, Elena Goun, Aleksey Yevtodiyenko

A system for monitoring biological processes in vivo is provided. The system comprises an implantable luciferase biosensor comprising luciferase in a biocompatible matrix and a caged luciferin probe. The caged luciferin probe can be administered to a livin ...

2018

Bioorthogonal chemistry in bioluminescence imaging

Aurélien Godinat, Elena Goun, Arkadiy Bazhin

Bioorthogonal chemistry has developed significant over the past few decades, to the particular benefit of molecular imaging. Bioluminescence imaging (BLI) along with other imaging modalities have significantly benefitted from this chemistry. Here, we revie ...

ELSEVIER SCI LTD2018

The Split Luciferin Reaction : From Bioorthogonal Chemistry to Bioluminescence Imaging

Aurélien Godinat

Studying biological processes on the level of live cells with the help of biocompatible reac-tions has tremendously advanced our understanding of basic biology. However, the great complexity of many human pathologies such as cancer, diabetes and neurodegen ...

EPFL2017

Precursor molecule for the synthesis of d-luciferin

Aurélien Godinat, Elena Goun

The present invention relates to precursor molecules for the synthesis of D-luciferin, or blocked D-luciferin and derivatives thereof, which are functionalized in different positions. Further, the use of functionalized precursor molecules for the synthesis ...

2015

Bioluminescence imaging of small biomolecules

Aurélien Godinat, Elena Goun, Riccardo Sinisi, Grigory Karateev, Aleksandra Konovalova, Jens Tomas Frigell, Ghyslain Martial Alain Budin

The invention relates to a technique to detect small molecules using Bioluminescence imaging (BLI) to image and quantify non-invasively, in vitro and in vivo,intracellular metabolite fluxes and which can be applied to azido-modified compounds, such as azid ...

2014

Live-cell multiplane three-dimensional super-resolution optical fluctuation imaging

Marcel Leutenegger, Theo Lasser, Aurélien Godinat, Françoise Gisou van der Goot Grunberg, Patrick Alain Sandoz, Noelia Laura Bocchio, Azat Sharipov, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) provides an elegant way of overcoming the diffraction limit in all three spatial dimensions by computing higher-order cumulants of image sequences of blinking fluorophores acquired with a classical widefi ...

Nature Research2014

A Biocompatible in Vivo Ligation Reaction and Its Application for Noninvasive Bioluminescent Imaging of Protease Activity in Living Mice

Aurélien Godinat, Douglas Hanahan, Ke Cheng, Gennady Nikitin

The discovery of biocompatible reactions had a tremendous impact on chemical biology, allowing the study of numerous biological processes directly in complex systems. However, despite the fact that multiple biocompatible reactions have been developed in th ...

Amer Chemical Soc2013

Cross coupling of non-activated alkyl halides with alkynyl Grignard reagents catalyzed by a well-defined Ni pincer complex

Rosario Scopelliti, Aurélien Godinat, Oleg Vechorkin, Xile Hu

The nickel pincer complex 1 catalyzes the cross-coupling of the title compounds with remarkable substrate scope and functional group tolerance. A nickel/alkynyl species was isolated and shown to be catalytically competent. THF=tetrahydrofuran, O-TMEDA=bis[ ...

Wiley-VCH Verlag GmbH2011

Covalent cell surface functionalization of human fetal osteoblasts for tissue engineering

Sandrine Gerber, Aurélien Godinat, Corinne Scaletta, Françoise Borcard, Davide Städler

The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne deriv ...

2011

Ruthenium-Catalyzed Synthesis of 1,5-Dichlorides by Sequential Intermolecular Kharasch Reactions

Kay Severin, Rosario Scopelliti, Aurélien Godinat, Katrin Anne Thommes, Charlotte Buron

Sequential intermolecular atom transfer radical addition reactions of activated dichlorides Cl2CRR′ (R = CN, CO2Et, R′ = H, CN, CO2Et) with two olefins catalyzed by [Cp*RuCl2(PPh3)] in the presence Mg allow the synthesis of linear 1,5-dichlorides. Differen ...

Wiley-Blackwell2011