Publications de Sarah Wulhfard

Valproic acid enhances recombinant mRNA and protein levels in transiently transfected Chinese hamster ovary cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Sarah Wulhfard

Valproic acid (VPA) is a small molecule that inhibits histone deacetylase activity. Here we report that VPA increases recombinant mRNA and protein levels in transiently transfected CHO DG44 cells. In the presence of VPA, transient recombinant antibody yiel ...

Elsevier2010

Transient recombinant protein expression in mammalian cells : the role of mRNA level and stability

Sarah Wulhfard

Transient gene expression (TGE) is a rapid method for generating recombinant proteins in mammalian cells, but the volumetric productivities for secreted proteins in transiently transfected CHO DG44 cells are typically more than an order of magnitude lower ...

EPFL2009

Mild hypothermia improves transient gene expression yields several fold in Chinese hamster ovary cells

Florian Maria Wurm, Sophie Bouchet, David Hacker, Stéphanie Tissot, Jean Cevey, Sarah Wulhfard

Large-scale transient gene expression (TGE) in mammalian cells is a rapid method to generate recombinant proteins, but the volumetric productivity for secreted proteins is still more than an order of magnitude lower than the yields typically achieved with ...

2008

Rational vector design and multi-pathway modulation of HEK 293E cells yield recombinant antibody titers exceeding 1 g/l by transient transfection under serum-free conditions

Florian Maria Wurm, Gaurav Backliwal, Sébastien Chenuet, Sarah Wulhfard

Transient transfection allows for fast production of recombinant proteins. However, the current bottlenecks in transient transfection are low titers and low specific productivity compared to stable cell lines. Here, we report an improved transient transfec ...

2008

Scalable transient gene expression in Chinese hamster ovary cells in instrumented and non-instrumented cultivation systems

Florian Maria Wurm, Martin Jordan, Madiha Derouazi, Natalie Muller, David Hacker, Sarah Wulhfard

Cell expansion, gene transfer and protein production were all executed with a single serum-free, animal protein-free commercial medium designed for suspension-adapted Chinese hamster ovary cells (CHO DG44). This is a most important process to consider for ...

Kluwer Academic Publishers, Springer2007