Publications de Corinne Berclaz

Label-free fast 3D coherent imaging reveals pancreatic islet micro-vascularization and dynamic blood flow

Theo Lasser, Anne Grapin-Botton, Corinne Berclaz, Paul James Marchand, David Nguyen, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann, Julia Sophie Katarina Nilsson

In diabetes, pancreatic beta-cells play a key role. These cells are clustered within structures called islets of Langerhans inside the pancreas and produce insulin, which is directly secreted into the blood stream. The dense vascularization of islets of La ...

2016

Longitudinal three-dimensional visualisation of autoimmune diabetes by functional optical coherence imaging

Theo Lasser, Martin Villiger, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Arno Pino Bouwens, Daniel Pawel Szlag, Jérôme Extermann

Aims/hypothesis It is generally accepted that structural and functional quantitative imaging of individual islets would be beneficial to elucidate the pathogenesis of type 1 diabetes. We here introduce functional optical coherence imaging (FOCI) for fast, ...

Springer Verlag2016

Combined Optical Coherence and Fluorescence Microscopy to assess dynamics and specificity of pancreatic beta-cell tracers

Theo Lasser, Antonio Lopez, Anne Grapin-Botton, Corinne Berclaz, Christophe Pache, Arno Pino Bouwens, Daniel Pawel Szlag

The identification of a beta-cell tracer is a major quest in diabetes research. However, since MRI, PET and SPECT cannot resolve individual islets, optical techniques are required to assess the specificity of these tracers. We propose to combine Optical Co ...

Nature Publishing Group2015

Structural and Functional Imaging of Pancreatic Islets using Optical Coherence Microscopy

Corinne Berclaz

In diabetes, pancreatic beta-cells play a key role. These cells are organized within structures called islets of Langerhans inside the pancreas and produce insulin. Insulin is one of the main hormones contributing to glucose homeostasis, which is the hallm ...

EPFL2015

Quantitative cerebral blood flow imaging with extended-focus optical coherence microscopy

Theo Lasser, Corinne Berclaz, Tristan Bolmont, Arno Pino Bouwens, Daniel Pawel Szlag

Quantitative three-dimensional blood flow imaging is a valuable technique to investigate the physiology of the brain. Two-photon microscopy (2PM) allows quantification of the local blood flow velocity with micrometric resolution by performing repeated line ...

Optical Society of America2014

Mapping molecular statistics with balanced super-resolution optical fluctuation imaging (bSOFI)

Marcel Leutenegger, Theo Lasser, Claudio Dellagiacoma, Corinne Berclaz, Noelia Laura Bocchio, Stefan Geissbuehler

Super-resolution optical fluctuation imaging (SOFI) achieves 3D super-resolution by computing temporal cumulants or spatio-temporal cross-cumulants of stochastically blinking fluorophores. In contrast to localization microscopy, SOFI is compatible with wea ...

2012

Diabetes imaging — quantitative assessment of islets of Langerhans distribution in murine pancreas using extended-focus optical coherence microscopy

Anthony Christopher Davison, Theo Lasser, Martin Villiger, Dimitri Nestor Alice Van De Ville, Anne Grapin-Botton, Corinne Berclaz, Joan Goulley, Erica Martin-Williams, Arno Pino Bouwens

Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Cohere ...

2012

Fast three-dimensional imaging of gold nanoparticles in living cells with photothermal optical lock-in Optical Coherence Microscopy

Christian Santschi, Theo Lasser, Martin Villiger, Corinne Berclaz, Christophe Pache, Matthew Gibson, Joan Goulley, Noelia Laura Bocchio, Arno Pino Bouwens

We introduce photothermal optical lock-in Optical Coherence Microscopy (poli-OCM), a volumetric imaging technique, which combines the depth sectioning of OCM with the high sensitivity of photothermal microscopy while maintaining the fast acquisition speed ...

Optical Society of America2012

Label-free imaging of cerebral β-amyloidosis with extended-focus optical coherence microscopy

Theo Lasser, Martin Villiger, Corinne Berclaz, Christophe Pache, Patrick Fraering, Tristan Bolmont, Mitko Dimitrov, Arno Pino Bouwens

We demonstrate label-free imaging of cerebral β-amyloidosis ex vivo and in a living mouse model of Alzheimer's disease using extended focus Fourier domain optical coherence microscopy (xfOCM). xfOCM provides 3D, high-resolution images of individual β-amylo ...

Society for Neuroscience2012