Publications de Diego Chiappe | EPFL Graph Search

The early communication stages between serine proteases and enterovirus capsids in the race for viral disintegration

Diego Chiappe, Tamar Kohn, Shannon Christa David, Shotaro Torii

Serine proteases are important environmental contributors of enterovirus biocontrol. However, the structural features of molecular interaction accounting for the susceptibility of enteroviruses to proteases remains unexplained. Here, we describe the molecu ...

Springer Science and Business Media LLC2024

Hypoxic Incubation Conditions for Optimized Manufacture of Tenocyte-Based Active Pharmaceutical Ingredients of Homologous Standardized Transplant Products in Tendon Regenerative Medicine

Diego Chiappe, Florence Armand, Romain Hamelin

Human fetal progenitor tenocytes (hFPT) produced in defined cell bank systems have recently been characterized and qualified as potential therapeutic cell sources in tendon regenerative medicine. In view of further developing the manufacture processes of s ...

MDPI2021

Analysis of the S. pombe Meiotic Proteome Reveals a Switch from Anabolic to Catabolic Processes and Extensive Post-transcriptional Regulation

Marc Moniatte, Diego Chiappe, Viesturs Simanis, Andrea Krapp, Elena Cano, Florence Armand, Romain Hamelin, Lucien Fabrice Krapp

Meiotic progression in S. pombe is regulated by stage-specific gene expression and translation, changes in RNA stability, expression of anti-sense transcripts, and targeted proteolysis of regulatory proteins. We have used SILAC labeling to examine the rela ...

2019

Beyond Unpredictability: The Importance of Reproducibility in Understanding the Protein Corona of Nanoparticles

Heinrich Hofmann, Sandra Caroline Galmarini, Diego Chiappe, Lionel André Maurizi, Nadia Rachel Von Moos, Noëlie Cayla

While it is well established that the surface of a nanoparticle plays a pivotal role for the protein corona, the vast number of proteins present in biological media render general conclusions about affinities between nanoparticle surfaces and proteins nont ...

AMER CHEMICAL SOC2018

Lysine acetylation stoichiometry and proteomics analyses reveal pathways regulated by sirtuin 1 in human cells

Diego Chiappe

Lysine acetylation is a widespread posttranslational modification affecting many biological pathways. Recent studies indicate that acetylated lysine residues mainly exhibit low acetylation occupancy, but challenges in sample preparation and analysis make i ...

Amer Soc Biochemistry Molecular Biology Inc2017

Protein Corona: Impact of Lymph Versus Blood in a Complex In Vitro Environment

Heinrich Hofmann, Ulrich Aschauer, Marc Moniatte, Diego Chiappe, Marijana Mionic Ebersold, Duncan Thomas Lindsay Alexander, Débora Bonvin

In biological environments, the surface of nanoparticles (NPs) are modified by protein corona (PC) that determines their biological behavior. Unfortunately, in vitro tests still give different PC than in vivo tests causing in vitro-in vivo discrepancy; hen ...

Wiley-Blackwell2017

Methods of protein corona isolation for magnetic nanoparticles

Heinrich Hofmann, Marc Moniatte, Diego Chiappe, Marijana Mionic Ebersold, Débora Bonvin

Nanoparticles (NPs) in contact with a biological environment get covered by proteins and some are loosely bound and some are tightly bound. The latter form a hard protein corona (HPC) which is known to determine their biological behavior. Therefore, in ord ...

Royal Soc Chemistry2017

An interplay between the p38 MAPK pathway and AUBPs regulates c-fos mRNA stability during mitogenic stimulation

Diego Chiappe, Tamara Tanos

Mitogen-activated protein kinase (MAPK) pathways constitute key regulatory elements linking extracellular stimuli to nuclear gene expression. Immediate-early responsive genes (IEGs) of the activator protein 1 (AP-1) family, such as fos, achieve peak expres ...

Portland Press Ltd2015

Quantitative Mass Spectrometry Reveals Plasticity of Metabolic Networks in Mycobacterium smegmatis

Marc Moniatte, Diego Chiappe, John McKinney, Tarun Chopra, Florence Armand, Romain Hamelin

Mycobacterium tuberculosis has a remarkable ability to persist within the human host as a clinically inapparent or chronically active infection. Fatty acids are thought to be an important carbon source used by the bacteria during long term infection. Catab ...

Amer Soc Biochemistry Molecular Biology Inc2014

Characterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens

Elena Suvorova Buffat, Diego Chiappe, Rizlan Bernier-Latmani, Elena Cristina Dalla Vecchia, Romain Hamelin, Paul Pao-Yun Shao

Desulfotomaculum reducens strain MI-1 is a Gram-positive, sulfate-reducing bacterium also capable of reducing Fe(III). Metal reduction in Gram-positive bacteria is poorly understood. Here, we investigated Fe(III) reduction with lactate, a non-fermentable s ...

Frontiers Media SA2014

A quantitative telomeric chromatin isolation protocol identifies different telomeric states

Joachim Lingner, Marc Moniatte, Diego Chiappe, Larissa Grolimund, Florence Armand, Romain Hamelin, Eric Aeby

Telomere composition changes during tumourigenesis, aging and in telomere syndromes in a poorly defined manner. Here we develop a quantitative telomeric chromatin isolation protocol (QTIP) for human cells, in which chromatin is cross-linked, immunopurified ...

Nature Publishing Group2013

Tissue Transglutaminase-mediated Glutamine Deamidation of beta-Amyloid Peptide Increases Peptide Solubility, Whereas Enzymatic Cross-linking and Peptide Fragmentation May Serve as Molecular Triggers for Rapid Peptide Aggregation

Horst Vogel, Enrico Condemi, Adrian Schmid, Marc Moniatte, Diego Chiappe, Yury Tsybin

Tissue transglutaminase (TGase) has been implicated in a number of cellular processes and disease states, where the enzymatic actions of TGase may serve in both, cell survival and apoptosis. To date, the precise functional properties of TGase in cell survi ...

American Society for Biochemistry and Molecular Biology2011

Phosphorylation at S87 is enhanced in synucleinopathies, inhibits alpha-synuclein oligomerization, and influences synuclein-membrane interactions

Patrick Aebischer, Bernard Schneider, Hilal Lashuel, Adrian Schmid, Marc Moniatte, Diego Chiappe, Abid Oueslati, Magdalena Zweckstetter

Increasing evidence suggests that phosphorylation may play an important role in the oligomerization, fibrillogenesis, Lewy body (LB) formation, and neurotoxicity of alpha-synuclein (alpha-syn) in Parkinson disease. Herein we demonstrate that alpha-syn is p ...

2010

Electron Capture and Transfer Dissociation: Peptide Structure Analysis at Different Ion Internal Energy Levels

Hisham Ben Hamidane, Marc Moniatte, Diego Chiappe, Yury Tsybin, Aleksey Vorobyev

We decoupled electron-transfer dissociation (ETD) and collision-induced dissociation of charge-reduced species (CRCID) events to probe the lifetimes of intermediate radical species in ETD-based ion trap tandem mass spectrometry of peptides. Short-lived int ...

Springer-Verlag2009