Publications de Corinne Scaletta

Industrial Biotechnology Conservation Processes: Similarities with Natural Long-Term Preservation of Biological Organisms

Nathalie Hirt-Burri, Philippe Abdel Sayed, Corinne Scaletta, Lee Ann Laurent-Applegate, Laurent Alexis Clément Vannay, Alexis Laurent

Cryopreservation and lyophilization processes are widely used for conservation purposes in the pharmaceutical, biotechnological, and food industries or in medical transplantation. Such processes deal with extremely low temperatures (e.g., -196 degrees C) a ...

MDPI2023

Bio-Enhanced Neoligaments Graft Bearing FE002 Primary Progenitor Tenocytes: Allogeneic Tissue Engineering & Surgical Proofs-of-Concept for Hand Ligament Regenerative Medicine

Sandra Jaccoud, Nathalie Hirt-Burri, Philippe Abdel Sayed, Corinne Scaletta, Lee Ann Laurent-Applegate, Laurent Alexis Clément Vannay, Joachim Meuli, Cédric Peneveyre, Alexis Laurent, Annick Jeannerat, Axelle Thomas

Hand tendon/ligament structural ruptures (tears, lacerations) often require surgical reconstruction and grafting, for the restauration of finger mechanical functions. Clinical-grade human primary progenitor tenocytes (FE002 cryopreserved progenitor cell so ...

MDPI2023

Autologous and Allogeneic Cytotherapies for Large Knee (Osteo)Chondral Defects: Manufacturing Process Benchmarking and Parallel Functional Qualification

Sandra Jaccoud, Nathalie Hirt-Burri, Philippe Abdel Sayed, Corinne Scaletta, Salim Elias Darwiche, Cédric Peneveyre, Alexis Laurent, Annick Jeannerat, Robin Martin

Cytotherapies are often necessary for the management of symptomatic large knee (osteo)-chondral defects. While autologous chondrocyte implantation (ACI) has been clinically used for 30 years, allogeneic cells (clinical-grade FE002 primary chondroprogenitor ...

MDPI2023

Development of Standardized Fetal Progenitor Cell Therapy for Cartilage Regenerative Medicine: Industrial Transposition and Preliminary Safety in Xenogeneic Transplantation

Dominique Pioletti, Sandra Jaccoud, Nathalie Hirt-Burri, Philippe Abdel Sayed, Corinne Scaletta, Salim Elias Darwiche, Alexis Laurent

Diverse cell therapy approaches constitute prime developmental prospects for managing acute or degenerative cartilaginous tissue affections, synergistically complementing specific surgical solutions. Bone marrow stimulation (i.e., microfracture) remains a ...

2021

Stability enhancement using hyaluronic acid gels for delivery of human fetal progenitor tenocytes

Dominique Pioletti, Alain Farron, Corinne Scaletta, Lee Ann Laurent-Applegate, Anthony Grognuz

Tendon afflictions are very common, and their negative impact is high both at the workplace and in leisure activities. Tendinopathies are increasing in prevalence and can lead to tendon ruptures, where healing is a long process with outcomes that are often ...

2016

Functionalization of microstructured open-porous bio-ceramic scaffolds with human fetal cells

Sandrine Gerber, Corinne Scaletta, Françoise Borcard, Davide Städler

Bone substitute materials allowing trans-scaffold migration and in-scaffold survival of human bone-derived cells are mandatory for development of cell-engineered permanent implants to repair bone defects. In this study, we evaluated the influence on human ...

American Chemical Society2012

Human epiphyseal chondro-progenitors provide a stable cell source for cartilage repair and regeneration

Dominique Pioletti, Corinne Scaletta

Wiley-Blackwell2012

Epiphyseal chondro-progenitors provide a stable cell source for cartilage cell therapy

Dominique Pioletti, Corinne Scaletta, Lee Ann Laurent-Applegate, Salim Elias Darwiche

Articular cartilage regeneration poses particularly tough challenges for implementing cell-‐based therapies. Many cell types have been investigated looking for a balanced combination of responsiveness and stability, yet techniques are still far from defin ...

2012

Human fetal bone cells in delivery systems for bone engineering

Dominique Pioletti, Sandra Jaccoud, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

The aim of this study was to culture human fetal bone cells (dedicated cell banks of fetal bone derived from 14 week gestation femurs) within both hyaluronic acid gel and collagen foam, to compare the biocompatibility of both matrices as potential delivery ...

Wiley-Blackwell2011

Biologicals and fetal cell therapy for wound healing and scar management

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

Few biopharmaceutical preparations developed from biologicals are available for tissue regeneration and scar management. When developing biological treatments with cellular therapy, selection of cell types and establishment of consistent cell banks are cru ...

2011

Covalent cell surface functionalization of human fetal osteoblasts for tissue engineering

Sandrine Gerber, Aurélien Godinat, Corinne Scaletta, Françoise Borcard, Davide Städler

The chemical functionalization of cell-surface proteins of human primary fetal bone cells with hydrophilic bioorthogonal intermediates was investigated. Toward this goal, chemical pathways were developed for click reaction-mediated coupling of alkyne deriv ...

2011

Plasticity of fetal cartilaginous cells

Dominique Pioletti, Sandra Jaccoud, Aurélie Quintin, Corinne Scaletta, Lee Ann Laurent-Applegate

Tissue-specific stem cells found in adult tissues can participate to the repair process following injury. However adult tissues, such as articular cartilage and intervertebral disc, have low regeneration capacity, whereas fetal tissues, such as articular c ...

2010

Chronic wound healing by fetal cell therapy may be explained by differential gene profiling observed in fetal versus old skin cells

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

Engineering of fetal tissue has a high potential for the treatment of acute and chronic wounds of the skin in humans as these cells have high expansion capacity under simple culture conditions and one organ donation can produce Master Cell Banks which can ...

2009

Isolation and in vitro chondrogenic potential of human fetal spine cells

Dominique Pioletti, Sandra Jaccoud, Aurélie Quintin, Corinne Scaletta, Lee Ann Laurent-Applegate

Cell therapy for nucleus pulposus regeneration is an attractive treatment for early disc degeneration as shown by studies using autologous nucleus pulposus cells or stem cells. Another potential source of cells is fetal cells. We investigated the feasibili ...

2009

Whole-cell bioprocessing of human fetal cells for tissue engineering of skin

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

Current restrictions for human cell-based therapies have been related to technological limitations with regards to cellular proliferation capacity (simple culture conditions), maintenance of differentiated phenotype for primary human cell culture and trans ...

2009

In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications

Dominique Pioletti, Corinne Scaletta, Lee Ann Laurent-Applegate

We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue engineering applications. Adult mesenchymal stem cells and adult osteoblasts were included in the study for comparison. Surface markers involved in b ...

2009

Wound healing gene-family expression differences between fetal and foreskin cells used for bioengineered skin substitutes

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

For tissue engineering, several cell types and tissues have been proposed as starting material. Allogenic skin products available for therapeutic usage are mostly developed with cell culture and with foreskin tissue of young individuals. Fetal skin cells o ...

2008

Human muscular fetal cells: a potential cell source for muscular therapies

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta

Myoblast transfer therapy has been extensively studied for a wide range of clinical applications, such as tissue engineering for muscular loss, cardiac surgery or Duchenne Muscular Dystrophy treatment. However, this approach has been hindered by numerous l ...

2008

Human fetal bone cells associated with ceramic reinforced PLA scaffolds for tissue engineering

Dominique Pioletti, Pierre-Etienne Bourban, Corinne Scaletta, Lee Ann Laurent-Applegate, Claire Diane Delabarde

Fetal bone cells were shown to have an interesting potential for therapeutic use in bone tissue engineering due to their rapid growth rate and their ability to differentiate into mature osteoblasts in vitro. We describe hereafter their capability to promot ...

2008

Consistency of fetal cell banks for research and clinical use

Dominique Pioletti, Aurélie Quintin, Corinne Scaletta, Lee Ann Laurent-Applegate

Current restrictions for human cell-based therapies have been related to technological limitations with regards to cellular proliferation capacity and maintenance of differentiated phenotype for primary human cell culture. In our experience, we have seen t ...

2007