Publications de Lee Ann Laurent-Applegate

Cartilage Repair: Promise of Adhesive Orthopedic Hydrogels

Dominique Pioletti, Lee Ann Laurent-Applegate, Peyman Karami

Cartilage repair remains a major challenge in human orthopedic medicine, necessitating the application of innovative strategies to overcome existing technical and clinical limitations. Adhesive hydrogels have emerged as promising candidates for cartilage r ...

MDPI2024

Bio-Enhanced Neoligaments Graft Bearing FE002 Primary Progenitor Tenocytes: Allogeneic Tissue Engineering & Surgical Proofs-of-Concept for Hand Ligament Regenerative Medicine

Sandra Jaccoud, Nathalie Hirt-Burri, Philippe Abdel Sayed, Corinne Scaletta, Lee Ann Laurent-Applegate, Laurent Alexis Clément Vannay, Joachim Meuli, Cédric Peneveyre, Alexis Laurent, Annick Jeannerat, Axelle Thomas

Hand tendon/ligament structural ruptures (tears, lacerations) often require surgical reconstruction and grafting, for the restauration of finger mechanical functions. Clinical-grade human primary progenitor tenocytes (FE002 cryopreserved progenitor cell so ...

MDPI2023

Industrial Biotechnology Conservation Processes: Similarities with Natural Long-Term Preservation of Biological Organisms

Nathalie Hirt-Burri, Philippe Abdel Sayed, Corinne Scaletta, Lee Ann Laurent-Applegate, Laurent Alexis Clément Vannay, Alexis Laurent

Cryopreservation and lyophilization processes are widely used for conservation purposes in the pharmaceutical, biotechnological, and food industries or in medical transplantation. Such processes deal with extremely low temperatures (e.g., -196 degrees C) a ...

MDPI2023

Loading Induced Self-Heating Of Articular Cartilage And Associated Signaling Mechanism

Dominique Pioletti, Lee Ann Laurent-Applegate, Naser Nasrollahzadeh Mamaghani

MARY ANN LIEBERT, INC2022

Efficient decellularization of equine tendon with preserved biomechanical properties and cytocompatibility for human tendon surgery indications

Dominique Pioletti, Nathalie Hirt-Burri, Lee Ann Laurent-Applegate, Anthony Grognuz, Cédric Peneveyre, Jens Antons, Pierre-Arnaud Aeberhard

Chronic and acute tendon injuries are frequent afflictions, for which treatment is often long and unsatisfactory. When facing extended injuries, matrices and scaffolds with sufficient biomechanical properties are required for surgical repair and could addi ...

2020

Depth-dependent mechanical properties of human articular cartilage obtained by indentation measurements

Pierre-Etienne Bourban, Lee Ann Laurent-Applegate, Matteo Gregorio Modesto Marascio, Jens Antons, Robin Martin

Emerging 3D printing technology permits innovative approaches to manufacture cartilage scaffolds associated with layer-by-layer mechanical property adaptation. However, information about gradients of mechanical properties in human articular cartilage is li ...

2018

Development of an effective cell seeding technique: simulation, implementation and analysis of contributing factors

Dominique Pioletti, Lee Ann Laurent-Applegate, Naser Nasrollahzadeh Mamaghani

Cell seeding in a biomaterial is an important process for tissue engineering applications. It helps to modulate tissue formation or to control initial conditions for mechanobiological studies. The compression release-induced suction (CRIS) seeding techniqu ...

Mary Ann Liebert, Inc2017

Human bone progenitor cells for clinical application: what kind of immune reaction does fetal xenograft tissue trigger in immuno-competent rats?

Dominique Pioletti, Tanja Cloé Hausherr, Lee Ann Laurent-Applegate

The potential of human fetal bone cells for successful bone regeneration has been shown in vivo. In particular, it has been demonstrated that the seeding of these cells in porous poly-(L-lactic acid)/ ß-tricalcium phosphate scaffolds improved the bone form ...

Cognizant Communication Corp2017

Stability enhancement using hyaluronic acid gels for delivery of human fetal progenitor tenocytes

Dominique Pioletti, Alain Farron, Corinne Scaletta, Lee Ann Laurent-Applegate, Anthony Grognuz

Tendon afflictions are very common, and their negative impact is high both at the workplace and in leisure activities. Tendinopathies are increasing in prevalence and can lead to tendon ruptures, where healing is a long process with outcomes that are often ...

2016

Anti-microbial dendrimers against multidrug-resistant P. aeruginosa enhance the angiogenic effect of biological burn-wound bandages

Dominique Pioletti, Jean-Louis Reymond, Philippe Abdel Sayed, Lee Ann Laurent-Applegate, Paris Jafari

Multi-drug resistant Pseudomonas aeruginosa has increased progressively and impedes further regression in mortality in burn patients. Such wound infections serve as bacterial reservoir for nosocomial infections and are associated with signi cant morbidity ...

Nature Research2016

Epiphyseal chondro-progenitors provide a stable cell source for cartilage cell therapy

Dominique Pioletti, Corinne Scaletta, Lee Ann Laurent-Applegate, Salim Elias Darwiche

Articular cartilage regeneration poses particularly tough challenges for implementing cell-‐based therapies. Many cell types have been investigated looking for a balanced combination of responsiveness and stability, yet techniques are still far from defin ...

2012

Biologicals and fetal cell therapy for wound healing and scar management

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

Few biopharmaceutical preparations developed from biologicals are available for tissue regeneration and scar management. When developing biological treatments with cellular therapy, selection of cell types and establishment of consistent cell banks are cru ...

2011

Human fetal bone cells in delivery systems for bone engineering

Dominique Pioletti, Sandra Jaccoud, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

The aim of this study was to culture human fetal bone cells (dedicated cell banks of fetal bone derived from 14 week gestation femurs) within both hyaluronic acid gel and collagen foam, to compare the biocompatibility of both matrices as potential delivery ...

Wiley-Blackwell2011

Regulation of proliferation and differentiation of human fetal bone cells

Dominique Pioletti, Lee Ann Laurent-Applegate

During the last decade, extensive research has been performed in the field of orthopedic medicine to develop cell-based therapies for the restoration of injured bone tissue. We previously demonstrated that human primary fetal bone cells (HFBCs) associated ...

2011

Plasticity of fetal cartilaginous cells

Dominique Pioletti, Sandra Jaccoud, Aurélie Quintin, Corinne Scaletta, Lee Ann Laurent-Applegate

Tissue-specific stem cells found in adult tissues can participate to the repair process following injury. However adult tissues, such as articular cartilage and intervertebral disc, have low regeneration capacity, whereas fetal tissues, such as articular c ...

2010

Whole-cell bioprocessing of human fetal cells for tissue engineering of skin

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

Current restrictions for human cell-based therapies have been related to technological limitations with regards to cellular proliferation capacity (simple culture conditions), maintenance of differentiated phenotype for primary human cell culture and trans ...

2009

In vitro characterization of immune-related properties of human fetal bone cells for potential tissue engineering applications

Dominique Pioletti, Corinne Scaletta, Lee Ann Laurent-Applegate

We describe herein some immunological properties of human fetal bone cells recently tested for bone tissue engineering applications. Adult mesenchymal stem cells and adult osteoblasts were included in the study for comparison. Surface markers involved in b ...

2009

Isolation and in vitro chondrogenic potential of human fetal spine cells

Dominique Pioletti, Sandra Jaccoud, Aurélie Quintin, Corinne Scaletta, Lee Ann Laurent-Applegate

Cell therapy for nucleus pulposus regeneration is an attractive treatment for early disc degeneration as shown by studies using autologous nucleus pulposus cells or stem cells. Another potential source of cells is fetal cells. We investigated the feasibili ...

2009

Chronic wound healing by fetal cell therapy may be explained by differential gene profiling observed in fetal versus old skin cells

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

Engineering of fetal tissue has a high potential for the treatment of acute and chronic wounds of the skin in humans as these cells have high expansion capacity under simple culture conditions and one organ donation can produce Master Cell Banks which can ...

2009

Wound healing gene-family expression differences between fetal and foreskin cells used for bioengineered skin substitutes

Dominique Pioletti, Nathalie Hirt-Burri, Corinne Scaletta, Lee Ann Laurent-Applegate

For tissue engineering, several cell types and tissues have been proposed as starting material. Allogenic skin products available for therapeutic usage are mostly developed with cell culture and with foreskin tissue of young individuals. Fetal skin cells o ...

2008