Publications de Seung Tae Lee | EPFL Graph Search

Targeted antibody and cytokine cancer immunotherapies through collagen affinity

Jeffrey Alan Hubbell, Melody Swartz, Seung Tae Lee, Lambert Charles François Potin, Jun Ishihara, Kazuto Fukunaga, Michal Mateusz Raczy

Cancer immunotherapy with immune checkpoint inhibitors (CPIs) and interleukin-2 (IL-2) has demonstrated clinical efficacy but is frequently accompanied with severe adverse events caused by excessive and systemic immune system activation. Here, we addressed ...

AMER ASSOC ADVANCEMENT SCIENCE2019

Difference in suitable mechanical properties of three-dimensional, synthetic scaffolds for self-renewing mouse embryonic stem cells of different genetic backgrounds

Jeffrey Alan Hubbell, Seung Tae Lee

We evaluated whether the genetic background of embryonic stem cells (ESCs) affects the properties suitable for three-dimensional (3D) synthetic scaffolds for cell self-renewal. Inbred R1 and hybrid B6D2F1 mouse ESC lines were cultured for 7 days in hydroge ...

Wiley2017

Culture of preantral follicles in poly(ethylene) glycol-based, three-dimensional hydrogel: a relationship between swelling ratio and follicular developments

Jeffrey Alan Hubbell, Seung Tae Lee

This study was undertaken to examine how the softness of poly(ethylene) glycol (PEG)-based hydrogels, creating a three-dimensional (3D) microenvironment, influences the in vitro growth of mouse ovarian follicles. Early secondary, preantral follicles of 2we ...

Wiley-Blackwell2015

Murine ovarian follicle culture in PEG-hydrogel: Effects of mechanical properties and the hormones FSH and LH on development

Jeffrey Alan Hubbell, Jong Eun Ihm, Seung Tae Lee

The development of an in vitro culture system, comparable to the in vivo environment in terms of effects on oocyte growth and development, could provide a valuable experimental tool for studying the mechanisms governing oocyte development as well as practi ...

Polymer Soc Korea2015

VEGFR-3 Neutralization Inhibits Ovarian Lymphangiogenesis, Follicle Maturation, and Murine Pregnancy

Jeffrey Alan Hubbell, Melody Swartz, Jong Eun Ihm, Joseph Rutkowski, Miriella Carmela Pasquier, Didier Trono, Seung Tae Lee, Witold Waldemar Kilarski, Vivianne Greenwood

Lymphatic vessels surround follicles within the ovary, but their roles in folliculogenesis and pregnancy, as well as the necessity of Lymphangiogenesis in follicle maturation and health, are undefined. We used systemic delivery of mF4-31C1, a specific anta ...

Elsevier Science Inc2013

A feeder-free, defined three-dimensional polyethylene glycol-based extracellular matrix niche for culture of human embryonic stem cells

Jeffrey Alan Hubbell, Seung Tae Lee, Jinhyun Kim

We report optimization of a serum- and feeder-free, three-dimensional (3D) niche created with a synthetic polyethylene glycol (PEG)-based extracellular matrix for self-renewal of human embryonic stem cells (hESCs). Three hESC lines (H9, H1 and Novo) were c ...

Elsevier Sci Ltd2013

Long-term maintenance of mouse embryonic stem cell pluripotency by manipulating integrin signaling within 3D scaffolds without active Stat3

Jeffrey Alan Hubbell, Stephane Kontos, Seung Tae Lee, Jungim Yun

We engineered an acellular biomimetic microenvironment to regulate stem cell fate and applied it to maintain mouse embryonic stem (ES) cell self-renewal. In the 3D environment formed using hydrogel scaffolds in which specific integrin ligation was provided ...

Elsevier Sci Ltd2012

Sorting Live Stem Cells Based on Sox2 mRNA Expression

Matthias Lütolf, Jeffrey Alan Hubbell, Peter Frey, Seung Tae Lee, Hans Mattias Larsson

While cell sorting usually relies on cell-surface protein markers, molecular beacons (MBs) offer the potential to sort cells based on the presence of any expressed mRNA and in principle could be extremely useful to sort rare cell populations from primary i ...

Public Library of Science2012

Engineering integrin signaling for promoting embryonic stem cell self-renewal in a precisely defined niche

Jeffrey Alan Hubbell, Jong Eun Ihm, Yun Suk Jo, Mayumi Mochizuki Martino, Stephane Kontos, Seung Tae Lee, Jungim Yun

We present development and use of a 3D synthetic extracellular matrix (ECM) analog with integrin- specific adhesion ligands to characterize the microenvironmental influences in embryonic stem cell (ESC) self-renewal. Transcriptional analysis of 24 integrin ...

Elsevier2010

Improved viability of freeze-thawed embryonic stem cells after exposure to glutathione

Seung Tae Lee

Adding a potent antioxidant, glutathione (GSH), to a cryoprotective solution consisting of dimethyl sulfoxide and ethylene glycol and/or postthaw culture medium significantly improved the postthaw viability of mouse embryonic stem cells. This effect, which ...

2010

Embryonic stem cell-like cells established by culture of adult ovarian cells in mice

Seung Tae Lee

Objective: To suggest an alternative strategy for deriving histocompatible stems cells without undertaking genetic manipulation. ...

2010

Change in gene expression of mouse embryonic stem cells derived from parthenogenetic activation

Seung Tae Lee

We previously established parthenogenetic mouse embryonic stem cells (ESCs) and this study was subsequently conducted for elucidating the influence of oocyte parthenogenesis on gene expression profile of ESCs. ...

Oxford University Press2009