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Yury Tsybin

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Publications associées (75)

Self-Assembly of a Giant Molecular Solomon Link from 30 Subcomponents

Kay Severin, Rosario Scopelliti, Clément Schouwey, Yury Tsybin, Konstantin Zhurov, Konstantin Nagornov

The synthesis of topologically complex structures, such as links and knots, is one of the current challenges in supramolecular chemistry. The so-called Solomon link consists of two doubly interlocked rings. Despite being a rather simple link from a topolog ...

Wiley-Blackwell2014

Empirical multi-dimensional space for scoring peptide spectrum matches in shotgun proteomics

Yury Tsybin, Unige Anna Laskay

Data-dependent tandem mass spectrometry (MS/MS) is one of the main techniques for protein identification in shotgun proteomics. In a typical LC MS/MS workflow, peptide product ion mass spectra (MS/MS spectra) are compared with those derived theoretically f ...

Amer Chemical Soc2014

Middle-down analysis of monoclonal antibodies with electron transfer dissociation Orbitrap FTMS

Yury Tsybin, Luca Fornelli, Daniel Ayoub

The rapid growth of approved biotherapeutics, e.g., monoclonal antibodies or immunoglobulins G (IgGs), demands improved techniques for their quality control. Traditionally, proteolysis-based bottom-up mass spectrometry (MS) has been employed. However, the ...

Amer Chemical Soc2014

High-Resolution Fourier Transform Ion Cyclotron Resonance Mass Spectrometry with Increased Throughput for Biomolecular Analysis

Yury Tsybin, Anton Kozhinov, Konstantin Nagornov

A multielectrode ion cyclotron resonance (ICR) cell, herein referred to as the 4X cell, for signal detection at the quadruple frequency multiple was implemented and characterized on a commercial 10 T Fourier transform ICR mass spectrometer (FT-ICR MS). Not ...

Amer Chemical Soc2014

Least-Squares Fitting of Time-Domain Signals for Fourier Transform Mass Spectrometry

Tagir Aushev, Yury Tsybin, Anton Kozhinov

To advance Fourier transform mass spectrometry (FTMS)-based molecular structure analysis, corresponding development of the FTMS signal processing methods and instrumentation is required. Here, we demonstrate utility of a least-squares fitting (LSF) method ...

Springer2014

From High- to Super-resolution Mass Spectrometry

Yury Tsybin

High-resolution mass spectrometry (MS) is indispensable for the molecular-level analysis of biological and environmental samples with great intra- and inter-molecular complexity. Here, we summarize developments in Fourier transform mass spectrometry (FTMS) ...

Schweizerische Chemische Gesellschaft2014

Advantages of Extended Bottom-Up Proteomics Using Sap9 for Analysis of Monoclonal Antibodies

Yury Tsybin, Luca Fornelli, Unige Anna Laskay, Kristina Srzentic, Daniel Ayoub

Despite the recent advances in structural analysis of monoclonal antibodies with bottom-up, middle-down, and top-down mass spectrometry (MS), further improvements in analysis accuracy, depth, and speed are needed. The remaining challenges include quantitat ...

Amer Chemical Soc2014

Evaluation of high-field Orbitrap Fourier transform mass spectrometer for petroleomics

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov

The performance of the high-field Orbitrap Fourier transform mass spectrometer (FTMS) is evaluated in the context of petroleum sample analysis. Pertinent characteristics including resolving power, mass accuracy, and spectral dynamic range are tested with r ...

Amer Chemical Soc2013

Practical Considerations for Improving the Productivity of Mass Spectrometry-based Proteomics

Yury Tsybin, Luca Fornelli, Anton Kozhinov, Oxana Upir, Unige Anna Laskay, Kristina Srzentic

Mass spectrometry (MS) is currently the most sensitive and selective analytical technique for routine peptide and protein structure analysis. Top-down proteomics is based on tandem mass spectrometry (MS/MS) of intact proteins, where multiply charged precur ...

Schweizerische Chemische Gesellschaft2013

Iterative Method for Mass Spectra Recalibration via Empirical Estimation of the Mass Calibration Function for Fourier Transform Mass Spectrometry-Based Petroleomics

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov

We describe a mass spectra recalibration method, which enables analysis of petroleum samples with Orbitrap FTMS. In this method, the mass calibration function is estimated on the basis of mass-to-charge ratios and abundances of internal calibrants without ...

Amer Chemical Soc2013

Hexagonal Class Representation for Fingerprinting and Facile Comparison of Petroleomic Samples

Yury Tsybin, Konstantin Zhurov, Anton Kozhinov

Because of the high complexity of petroleomic-type samples, there is a need for efficient ways of visualizing and interpreting the resulting data in mass spectrometry-based petroleomics. Over the years, several graphing approaches have become widespread, y ...

Amer Chemical Soc2013

Top-down analysis of 30–80 kDa proteins by electron transfer dissociation time-of-flight mass spectrometry

Yury Tsybin, Luca Fornelli, Julien Parra

Electron transfer dissociation (ETD)-based top-down mass spectrometry (MS) is the method of choice for in-depth structure characterization of large peptides, small- and medium-sized proteins, and non-covalent protein complexes. Here, we describe the perfor ...

Springer Berlin Heidelberg2013

Principles of electron capture and transfer dissociation mass spectrometry applied to peptide and protein structure analysis

Yury Tsybin, Konstantin Zhurov, Matthew Wodrich, Luca Fornelli, Unige Anna Laskay

This tutorial review describes the principles and practices of electron capture and transfer dissociation (ECD/ETD or ExD) mass spectrometry (MS) employed for peptide and protein structure analysis. ExD MS relies on interactions between gas phase peptide o ...

Royal Society of Chemistry2013

Proteome Digestion Specificity Analysis for Rational Design of Extended Bottom-up and Middle-down Proteomics Experiments

Yury Tsybin, Unige Anna Laskay, Kristina Srzentic

Mass spectrometry (MS)-based bottom-up proteomics (BUP) is currently the method of choice for large-scale identification and characterization of proteins present in complex samples, such as cell lysates, body fluids, or tissues. Technically, BUP relies on ...

Amer Chemical Soc2013

Sidebands in Fourier transform ion cyclotron resonance mass spectra

Yury Tsybin, Anton Kozhinov, Sasa Miladinovic

Sidebands in mass spectra are an intrinsic feature of Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS). Appearance of the sidebands there is detrimental for the analytical performance, especially in case of complex mixtures analyzed ...

Elsevier2012

Analysis of Intact Monoclonal Antibody IgG1 by Electron Transfer Dissociation Orbitrap FTMS

Yury Tsybin, Luca Fornelli

The primary structural information of proteins employed as biotherapeutics is essential if one wishes to understand their structure-function relationship, as well as in the rational design of new therapeutics and for quality control. Given both the large s ...

Amer Soc Biochemistry Molecular Biology Inc2012

Maleimide-functionalised organoruthenium anticancer agents and their binding to thiol-containing biomolecules

Paul Joseph Dyson, Yury Tsybin, Christian Hartinger, Alexey Nazarov, Michael Groessl, Muhammad Hanif

Ru-II(arene) anticancer compounds with maleimide functionality were prepared to allow selective interaction with thiol-containing biomolecules and thereby enforcing the selective delivery of the compounds to the tumour. ...

Royal Society of Chemistry2012

Observation of ion coalescence in Orbitrap Fourier transform mass spectrometry

Yury Tsybin, Luca Fornelli

RATIONALE Similar to other mass spectrometric technologies based on ion trapping in a spatially restricted area, the performance of Orbitrap Fourier transform mass spectrometry (FTMS) is affected by the interaction between the trapped ion clouds. One of th ...

Wiley-Blackwell2012

Fragmentation methods on the balance: unambiguous top–down mass spectrometric characterization of oxaliplatin–ubiquitin binding sites

Paul Joseph Dyson, Yury Tsybin, Christian Hartinger

The interaction between oxaliplatin and the model protein ubiquitin (Ub) was investigated in a top-down approach by means of high-resolution electrospray ionization mass spectrometry (ESI-MS) using diverse tandem mass spectrometric (MS/MS) techniques, incl ...

Springer-Verlag2012

Statistical Analysis of Ion Mobility Spectrometry. II. Adaptively Biased Methods and Shape Correlations

Yury Tsybin

Following a recent effort [J. Am. Soc. Mass Spectrom. 23, 386-396 (2012)], we continue to explore computational methodologies for generating molecular conformations to support collisional cross sections suggested by ion mobility measurements. Here, adaptiv ...

2012

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