Publications de Chiara Gabella

Quantitative Assessment of Research Data Management Practices - 2023

This survey investigates Research Data Management (RDM) practices across five Swiss higher education institutions, including EPFL, ETH Zürich, Eawag, FHNW, and DaSCH, with the goal of gathering insights into how researchers manage data and code throughout ...

Zenodo2024

Quantitative assessment of research data management practice - 2021

Chiara Gabella, Francesco Varrato, Eliane Ninfa Blumer

This survey aims to investigate research data management practices at EPFL and integrate their results into specific academic services. The previous two editions, in collaboration with TU Delft, Cambridge University and Illinois University, were carried ou ...

2022

Minimal model for spontaneous cell polarization and edge activity in oscillating, rotating and migrating cells

Jean-Jacques Meister, Alexander Verkhovskiy, Mark Etienne Ambühl, Chiara Gabella, Franck Claude Raynaud, Alicia Carine Bornert

How cells break symmetry and organize activity at their edges to move directionally is a fundamental question in cell biology. Physical models of cell motility commonly incorporate gradients of regulatory proteins and/or feedback from the motion itself to ...

Nature Publishing Group2016

Microsurgery-aided in-situ force probing reveals extensibility and viscoelastic properties of individual stress fibers

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Benoît Vianay, Céline Jeanne Labouesse

Actin-myosin filament bundles (stress fibers) are critical for tension generation and cell shape, but their mechanical properties are difficult to access. Here we propose a novel approach to probe individual peripheral stress fibers in living cells through ...

Nature Research2016

Cell Shape Dynamics Reveal Balance of Elasticity and Contractility in Peripheral Arcs

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Benoît Vianay, Céline Jeanne Labouesse

The mechanical interaction between adherent cells and their substrate relies on the formation of adhesion sites and on the stabilization of contractile acto-myosin bundles, or stress fibers. The shape of the cell and the orientation of these fibers can be ...

Cell Press2015

Ultra-soft cantilevers and 3-D micro-patterned substrates for contractile bundle tension measurement in living cells

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Niccolo Piacentini, Benoît Vianay

Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far dif ...

Royal Society of Chemistry2014

Contact Angle at the Leading Edge Controls Cell Protrusion Rate

László Forró, Jean-Jacques Meister, Alexander Verkhovskiy, Sylvia Jeney, Chiara Gabella, Elena Bertseva, Niccolo Piacentini, Alicia Carine Bornert

Plasma membrane tension and the pressure generated by actin polymerization are two antagonistic forces believed to define the protrusion rate at the leading edge of migrating cells [1-5]. Quantitatively, resistance to actin protrusion is a product of membr ...

Elsevier2014

Interplay between cytoskeletal forces, membrane tension and shape in rapidly migrating cells

Chiara Gabella

Cell migration is a fundamental process in all animal cells. The ability of cell to migrate is crucial for many pathological and physiological conditions such as morphogenesis, the immune response to inflammatory or vascular diseases and cancer metastasis. ...

EPFL2013

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

Interplay between cytoskeletal forces, membrane tension, and hydrostatic pressure in rapidly migrating cells

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Elena Bertseva

Amer Soc Cell Biology2011