Publications de Ana Valero | EPFL Graph Search

Dielectrophoresis as a single cell characterization method for bacteria

Philippe Renaud, Thomas Michael Braschler, John McKinney, Neeraj Dhar, Ana Valero

Real-time, quantitative characterization of cells at single-cell resolution, particularly while maintain- ing their intrinsic properties and without affecting cellular processes, is of primary importance in modern biological assays. Dielectrophoresis is a ...

Iop Publishing Ltd2017

On-Chip Flow Cytometry

Philippe Renaud, Ana Valero

Springer Netherlands2016

On-Chip Flow Cytometry

Philippe Renaud, Thomas Michael Braschler, Ana Valero

Springer2012

Tracking and synchronization of the yeast cell cycle using dielectrophoretic opacity

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler, Ana Valero

Cell cycle synchronization is an important tool for the study of the cell division stages and signalling. It provides homogeneous cell cultures that are of importance to develop and improve processes such as protein synthesis and drug screening. The main a ...

Royal Society of Chemistry2011

Synergistic NGF/B27 Gradients Position Synapses Heterogeneously in 3D Micropatterned Neural Cultures

Philippe Renaud, Dominique Zosso, Anja Kunze, Ana Valero

Native functional brain circuits show different numbers of synapses (synaptic densities) in the cerebral cortex. Until now, different synaptic densities could not be studied in vitro using current cell culture methods for primary neurons. Herein, we presen ...

Public Library of Science2011

Micropatterning neural cell cultures in 3D with a multi-layered scaffold

Philippe Renaud, Anja Kunze, Michele Giugliano, Ana Valero

Cortical neurons, in their native state, are organized in six different cell layers; and the thickness of the cell layer ranges from 0.12 mm to 0.4 mm. The structure of cell layers plays an important role in neurodegenerative diseases or corticogenesis. We ...

2011

Link between Alginate Reaction Front Propagation and General Reaction Diffusion Theory

Philippe Renaud, Jürgen Brugger, Thomas Michael Braschler, Kristopher Pataky, Ana Valero, Ludovica Colella

We provide a common theoretical framework reuniting specific models for the Ca2+-alginate system and general reaction diffusion theory along with experimental validation on a microfluidic chip. As a starting point, we use a set of nonlinear, partial differ ...

2011

A unified approach to dielectric single cell analysis: Impedance and dielectrophoretic force spectroscopy

Philippe Renaud, Thomas Michael Braschler, Ana Valero

In this review we present a unified approach for single cell dielectric spectroscopy. Impedance spectroscopy and dielectrophoretic cell sorting, current microtechnologies applied in electrical analysis of single cells are discussed based on their closely r ...

Royal Society of Chemistry2010

Fluidic microstructuring of alginate hydrogels for the single cell niche

Philippe Renaud, Jürgen Brugger, Thomas Michael Braschler, Kristopher Pataky, Ana Valero, Ludovica Colella

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible co ...

Royal Society of Chemistry2010

Optimization of microfluidic single cell trapping for long-term on-chip culture

Matthias Lütolf, Philippe Renaud, Jonas Marcel Lätt, Stefan Kobel, Ana Valero

The poor efficiency of microfluidic single cell trapping is currently restricting the full potential of state-of-the-art single cell analyses. Using fluid dynamics simulations in combination with particle image velocimetry to systematically optimize trap a ...

Royal Society of Chemistry2010

A miniaturized continuous dielectrophoretic cell sorter and its applications

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler, Ana Valero

There is great interest in highly sensitive separation methods capable of quickly isolating a particular cell type within a single manipulation step prior to their analysis. We present a cell sorting device based on the opposition of dielectrophoretic forc ...

American Institute of Physics2010

Efficient high-thoughput microfluidic trapping of single cells

Matthias Lütolf, Philippe Renaud, Ana Valero

2009

Sequential build-up of hydrogel environments around single cells

Philippe Renaud, Joël Theytaz, Thomas Michael Braschler, Anja Kunze, Grazia Loche, Ana Valero, Ludovica Colella

Controlling alginate gel formation by diffusion of Ca ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It gives the possibility of exposing cells under biocompatible condi ...

2009

Rapid Immunoassay Using Steady-State Dispersion Effects in Nanochannels

Philippe Renaud, Nicolas Durand, Ana Valero

We present a novel method for on-chip competitive dispersion immunoassay (DISIA) which aims at measuring the specificity between fluorescently-labeled antibodies (Ab*) and label-free antigens (Ag). We have fabricated a nanofluidic device that allows fast d ...

2008