Publications de Juan Francisco Bada Juarez

Exploring New Nanopore Sensors from the Aerolysin Family

Juan Francisco Bada Juarez, Matteo Dal Peraro, Maria Josefina Marcaida Lopez

Aerolysin‐like proteins are a family of pore‐forming toxins with potential biotechnological applications as nanopore sensors for biomolecular detection and sequencing. Despite their conserved structural fold, the low sequence identity complicates sequence ...

Wiley2025

Aerolysin Nanopore Structures Revealed at High Resolution in a Lipid Environment

Juan Francisco Bada Juarez, Matteo Dal Peraro, Luciano Andres Abriata, Maria Josefina Marcaida Lopez, Jana Susanne Anton

Aerolysin is a beta-pore-forming toxin produced by most Aeromonas bacteria, which has attracted large attention in the field of nanopore sensing due to its narrow and charged pore lumen. Structurally similar proteins, belonging to the aerolysin-like family ...

AMER CHEMICAL SOC2025

Enhanced Analytes Capture by Engineering Electrostatics at the Entry of Aerolysin Nanopore

Juan Francisco Bada Juarez

Nanopore technology is a powerful single‐molecule platform for detecting and sequencing a wide range of biomolecules. Among nanopores, aerolysin has emerged as a particularly promising candidate for peptide sensing. However, its ability to capture long bio ...

Wiley2025

Using integrative structural biology for the identification of novel aerolysin-like pore forming toxins for nanopore sensing

Juan Francisco Bada Juarez, Matteo Dal Peraro, Maria Josefina Marcaida Lopez, Fernando Augusto Teixeira Pinto Meireles, Jana Susanne Anton

SPRINGER2023

A versatile approach to high-density microcrystals in lipidic cubic phase for room-temperature serial crystallography

Juan Francisco Bada Juarez

Serial crystallography has emerged as an important tool for structural studies of integral membrane proteins. The ability to collect data from micrometre-sized weakly diffracting crystals at room temperature with minimal radiation damage has opened many ne ...

2023

Deep Learning-Assisted Single-Molecule Detection of Protein Post-translational Modifications with a Biological Nanopore

Hilal Lashuel, Anass Chiki, Juan Francisco Bada Juarez, Matteo Dal Peraro, Lucien Fabrice Krapp, Chan Cao, Simon Finn Mayer, Pedro José Santana Ribeiro Magalhães

Protein post-translational modifications (PTMs) play a crucial role in countless biological processes, profoundly modulating protein properties on both spatial and temporal scales. Protein PTMs have also emerged as reliable biomarkers for several diseases. ...

Amer Chemical Soc2023