Publications de Ludovica Colella

An impedance-based flow microcytometer for single cell morphology discrimination

Philippe Renaud, Ludovica Colella, Marjan Shaker

Cell shape is a fundamental biological feature, providing specific information about physiological or pathological cellular conditions. Most of the state-of-the-art microfluidic cytometers, however, only allow simple cell analysis, including viability stud ...

Royal Soc Chemistry2014

Modeling, Simulation, and Performance Evaluation of a Novel Microfluidic Impedance Cytometer for Morphology-Based Cell Discrimination

Philippe Renaud, Ludovica Colella, Marjan Shaker

The performance of a novel microfluidic impedance cytometer [1] for single-cell analysis is investigated in-silico by means of a finite element model. The main feature of the device is the ability to probe impedance of flowing cells along two orthogonal di ...

Institute of Electrical and Electronics Engineers2014

Microelectrode-based dielectric spectroscopy of glucose effect on erythrocytes

Philippe Renaud, Ludovica Colella

The dielectric response of biconcave erythrocytes exposed to D-glucose and I.-glucose has been investigated using a double array of planar interdigitated microelectrodes on a glass microchip. Erythrocytes are analyzed under physiological conditions suspend ...

2012

On chip cellular dielectric spectroscopy : monitoring of glucose effect on erythrocytes membrane and analysis of the physiological role of anthrax toxin receptor in endothelial cells

Ludovica Colella

In the recent years, the development of automated systems for cellular monitoring has been highly promoted in basic biological research as well as in industries and medical diagnostic. Several methods for transduction of cellular signals have been investig ...

EPFL2012

Link between Alginate Reaction Front Propagation and General Reaction Diffusion Theory

Philippe Renaud, Jürgen Brugger, Thomas Michael Braschler, Kristopher Pataky, Ana Valero, Ludovica Colella

We provide a common theoretical framework reuniting specific models for the Ca2+-alginate system and general reaction diffusion theory along with experimental validation on a microfluidic chip. As a starting point, we use a set of nonlinear, partial differ ...

2011

Fluidic microstructuring of alginate hydrogels for the single cell niche

Philippe Renaud, Jürgen Brugger, Thomas Michael Braschler, Kristopher Pataky, Ana Valero, Ludovica Colella

Controlling alginate gel formation by diffusion of Ca2+ ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It offers the possibility of exposing cells under biocompatible co ...

Royal Society of Chemistry2010

Sequential build-up of hydrogel environments around single cells

Philippe Renaud, Joël Theytaz, Thomas Michael Braschler, Anja Kunze, Grazia Loche, Ana Valero, Ludovica Colella

Controlling alginate gel formation by diffusion of Ca ions through a filter barrier, a layer-by-layer deposition technique with resolution on the size scale of a single cell is presented. It gives the possibility of exposing cells under biocompatible condi ...

2009