Publications de Lara Buscemi Estefanell

Tracking Hyperpolarized [1-¹³C] Pyruvate and [1-¹³C] L-Lactate Metabolism in the Healthy and Post-Stroke Mouse Brain

Bernard Lanz, Rolf Gruetter, Elise Marie Catherine Vinckenbosch, Lara Buscemi Estefanell, Mor-Miri Mishkovsky, Mario Gaetano Lepore, Jean-Noël Hyacinthe, Thanh Phong Kevin Lê

Tracking hyperpolarized (HP) ¹³C labeling from either [1-¹³C] pyruvate or [1-¹³C] lactate is a useful tool to assess intermediary metabolism in vivo, which has already been translated from preclinical to clinical research. HP [1-¹³C] pyruvate and [1-¹³C] l ...

Wiley2025

Influence of DNP Polarizing Agents on Biochemical Processes: TEMPOL in Transient Ischemic Stroke

Lara Buscemi Estefanell, Mor-Miri Mishkovsky, Mario Gaetano Lepore, Jean-Noël Hyacinthe, Thanh Phong Kevin Lê

Hyperpolarization of 13C by dissolution dynamic nuclear polarization (dDNP) boosts the sensitivity of magnetic resonance spectroscopy (MRS), making possible the monitoring in vivo and in real time of the biochemical reactions of exogenously infused 13C-lab ...

2023

Metabolic fingerprints discriminating severity of acute ischemia using in vivo high-field H-1 magnetic resonance spectroscopy

Hongxia Lei, Lara Buscemi Estefanell, Mario Gaetano Lepore

Despite the improving imaging techniques, it remains challenging to produce magnetic resonance (MR) imaging fingerprints depicting severity of acute ischemia. The aim of this study was to evaluate the potential of the overall high-field H-1 MR Spectroscopy ...

Wiley-Blackwell2020

Evaluating the potential of hyperpolarised [1-13C] L-lactate as a neuroprotectant metabolic biosensor for stroke

Lara Buscemi Estefanell, Mor-Miri Mishkovsky, Mario Gaetano Lepore, Jean-Noël Hyacinthe, Thanh Phong Kevin Lê

Cerebral metabolism, which can be monitored by magnetic resonance spectroscopy (MRS), changes rapidly after brain ischaemic injury. Hyperpolarisation techniques boost (13)C MRS sensitivity by several orders of magnitude, thereby enabling in vivo monitoring ...

Nature Research2020

Non-invasive diagnostic biomarkers for estimating the onset time of permanent cerebral ischemia

Rolf Gruetter, Lijing Xin, Hongxia Lei, Corinne Benakis, Lara Buscemi Estefanell

The treatments for ischemic stroke can only be administered in a narrow time-window. However, the ischemia onset time is unknown in ~30% of stroke patients (wake-up strokes). The objective of this study was to determine whether MR spectra of ischemic brain ...

Nature Publishing Group2014

The Nano-Scale Mechanical Properties of the Extracellular Matrix Regulate Dermal Fibroblast Function

Jean-Jacques Meister, Boris Hinz, Josiane Angèle Charlotte Smith-Clerc, Lara Buscemi Estefanell

Changes in the mechanical properties of dermis occur during skin aging or tissue remodeling and affect the activity of resident fibroblasts. With the aim to establish elastic culture substrates that reproduce the variable softness of dermis, we determined ...

Nature Publishing Group2014

Identification and functional response of interstitial Cajal-like cells from rat mesenteric artery

Jean-Jacques Meister, Jean-Louis Bény, Aurélie Formey de Saint Louvent, Lara Buscemi Estefanell

Cells with irregular shapes, numerous long thin filaments, and morphological similarities to the gastrointestinal interstitial cells of Cajal (ICCs) have been observed in the wall of some blood vessels. These ICC-like cells (ICC-LCs) do not correspond to t ...

Springer-Verlag2011

Dynamic measurement of the height and volume of migrating cells by a novel fluorescence microscopy technique

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Lara Buscemi Estefanell, Benoît Vianay

We propose a new technique to measure the volume of adherent migrating cells. The method is based on a negative staining where a fluorescent, non-cell-permeant dye is added to the extracellular medium. The specimen is observed with a conventional fluoresce ...

2011

The Single-Molecule Mechanics of the Latent TGF-beta 1 Complex

Jean-Jacques Meister, Boris Hinz, Josiane Angèle Charlotte Smith-Clerc, Aurélie Formey de Saint Louvent, Lara Buscemi Estefanell, David Ramonet Jimenez

Background: TGF-beta 1 controls many pathophysiological processes including tissue homeostasis, fibrosis, and cancer progression. Together with its latency-associated peptide (LAP), TGF-beta 1 binds to the latent TGF-beta 1-binding protein-1 (LTBP-1), whic ...

Elsevier2011

A New Lock-step Mechanism of Matrix Remodelling Based on Subcellular Contractile Events

Jean-Jacques Meister, Lysianne Follonier Castella, Boris Hinz, Lara Buscemi Estefanell

Myofibroblasts promote tissue contractures during fibrotic diseases. To understand how spontaneous changes in the intracellular calcium concentration, Ca2+, contribute to myofibroblast contraction, we analysed both Ca2+ and subcellular contractio ...

Company of Biologists2010

The covalent attachment of adhesion molecules to silicone membranes for cell stretching applications

Hicham Majd, Jean-Jacques Meister, Boris Hinz, Pierre Jean Arnould Wipff, Lara Buscemi Estefanell

Strain devices with expandable polydimethylsiloxane (PDMS) culture membranes are frequently used to stretch cells in vitro, mimicking mechanically dynamic tissue environments. To immobilize cell-adhesive molecules to the otherwise non-adhesive PDMS substra ...

Elsevier2009

A novel method of dynamic culture surface expansion improves mesenchymal stem cell proliferation and phenotype

Hicham Majd, Boris Hinz, Pierre Jean Arnould Wipff, Lara Buscemi Estefanell

Repeated passaging in conventional cell culture reduces pluripotency and proliferation capacity of human mesenchymal stem cells (MSC). We introduce an innovative cell culture method whereby the culture surface is dynamically enlarged during cell proliferat ...

2008