Publications de Sandra Eloise Kjeldsen

Cancer cells subvert the primate-specific KRAB zinc finger protein ZNF93 to control APOBEC3B

Didier Trono, Priscilla Turelli, Julien Léonard Duc, Sandra Eloise Kjeldsen, Charlène Mireille Raymonde Raclot, Cyril David Son-Tuyên Pulver, Evaristo Jose Planet Letschert, Filipe Amândio Brandão Sanches Vong Martins, Olga Marie Louise Rosspopoff, Romain Forey

ZNF93 is a primate-restricted Krüppel-associated box zinc finger protein responsible for repressing 20- to 12-My-old L1 transposable elements. Here, we reveal that ZNF93 also regulates the key cancer driver APOBEC3B—a mutagenic enzyme linked to tumorigenes ...

Proceedings of the National Academy of Sciences2025

Evolutionarily recent transcription factors partake in human cell cycle regulation

Didier Trono, Sandra Eloise Kjeldsen, Charlène Mireille Raymonde Raclot, Alexandre Coudray, Cyril David Son-Tuyên Pulver, Evaristo Jose Planet Letschert, Filipe Amândio Brandão Sanches Vong Martins, Alex Russell Lederer, Olga Marie Louise Rosspopoff, Romain Forey

The cell cycle is a fundamental process in eukaryotic biology and is accordingly controlled by a highly conserved core signaling cascade. However, whether recently evolved proteins also influence this process is unclear. Here, we systematically map the inf ...

2025

Pou5f1b inhibitors

Didier Trono, Françoise Gisou van der Goot Grunberg, Laurence Gouzi Abrami, Sandra Eloise Kjeldsen, Laia Simo Riudalbas

The invention provides an agent modulating the expression and/or activity of i) the POU5F1B protein, a fragment or variant thereof, ii) an mRNA encoding POU5F1B, a fragment or variant thereof and/or iii) the POU5F1B gene. Preferably, the agent is for use i ...

2024

Clusters of lineage-specific genes are anchored by ZNF274 in repressive perinucleolar compartments

Didier Trono, Julien Léonard Duc, Sandra Eloise Kjeldsen, Shaoline Sheppard, Delphine Yvette L Grun, Olga Marie Louise Rosspopoff

Long known as the site of ribosome biogenesis, the nucleolus is increasingly recognized for its role in shaping three-dimensional (3D) genome organization. Still, the mechanisms governing the targeting of selected regions of the genome to nucleolus-associa ...

American Association for the Advancement of Science (AAAS)2024

A Cluster of Evolutionarily Recent KRAB Zinc Finger Proteins Protects Cancer Cells from Replicative Stress–Induced Inflammation

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Cyril David Son-Tuyên Pulver, Evaristo Jose Planet Letschert, Filipe Amândio Brandão Sanches Vong Martins, Olga Marie Louise Rosspopoff, Joana Carlevaro Fita, Romain Forey, Florian Huber

Heterochromatin loss and genetic instability enhance cancer progression by favoring clonal diversity, yet uncontrolled replicative stress leads to mitotic catastrophe and inflammatory responses that promote immune rejection. KRAB domain-containing zinc fin ...

2024

Genetic features and genomic targets of human KRAB-zinc finger proteins

Didier Trono, Priscilla Turelli, Julien Léonard Duc, Sandra Eloise Kjeldsen, Jonas Caspar De Tribolet-Hardy, Bara Khubieh, Jacques Fellay, Michaël Imbeault, Alexandre Coudray, Cyril David Son-Tuyên Pulver, Evaristo Jose Planet Letschert, Christian Axel Wandall Thorball, Romain Forey

Krüppel-associated box (KRAB) domain-containing zinc finger proteins (KZFPs) are one of the largest groups of transcription factors encoded by tetrapods, with 378 members in human alone. KZFP genes are often grouped in clusters reflecting amplification by ...

2023

Primate-specific transposable elements shape transcriptional networks during human development

Matthias Lütolf, Didier Trono, Julien Léonard Duc, Sandra Eloise Kjeldsen, Cyril David Son-Tuyên Pulver, Evaristo Jose Planet Letschert, Andrea Manfrin, Julien Paul André Pontis, Delphine Yvette L Grun, Christopher James Playfoot

The human genome harbors more than 4.5 million transposable element (TE)-derived insertions, the result of recurrent waves of invasion and internal propagation. Here they show that TEs belonging to evolutionarily recent subfamilies go on to regulate later ...

Nature Portfolio2022

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

Didier Trono, Laurence Gouzi Abrami, Julien Léonard Duc, Sandra Eloise Kjeldsen, Alexandre Coudray, Sagane Dind, Evaristo Jose Planet Letschert, Laia Simo Riudalbas

The treatment of colorectal cancer (CRC) is an unmet medical need in absence of early diagnosis. Here, upon characterizing cancer-specific transposable element-driven transpochimeric gene transcripts (TcGTs) produced by this tumor in the SYSCOL cohort, we ...

Nature Research2022

Transposon-activated POU5F1B promotes colorectal cancer growth and metastasis

Didier Trono, Laurence Gouzi Abrami, Julien Léonard Duc, Sandra Eloise Kjeldsen, Alexandre Coudray, Sagane Dind, Evaristo Jose Planet Letschert, Laia Simo Riudalbas

AMER ASSOC CANCER RESEARCH2021

Hominoid-Specific Transposable Elements and KZFPs Facilitate Human Embryonic Genome Activation and Control Transcription in Naive Human ESCs

Didier Trono, Priscilla Turelli, Julien Léonard Duc, Sandra Eloise Kjeldsen, Julien Duc, Alexandre Coudray, Evaristo Jose Planet Letschert, Julien Paul André Pontis

Expansion of transposable elements (TEs) coincides with evolutionary shifts in gene expression. TEs frequently harbor binding sites for transcriptional regulators, thus enabling coordinated genome-wide activation of species- and context-specific gene expre ...

2019

ZNF445 is a primary regulator of genomic imprinting

Didier Trono, Priscilla Turelli, Sandra Eloise Kjeldsen, Michaël Imbeault, Andrea Coluccio, Evaristo Jose Planet Letschert, Christian Axel Wandall Thorball

Genomic imprinting is an epigenetic process regulated by germline-derived DNA methylation, causing parental origin-specific monoallelic gene expression. Zinc finger protein 57 (ZFP57) is critical for maintenance of this epigenetic memory during post-fertil ...

2019

DUX is a non-essential synchronizer of zygotic genome activation

Didier Trono, Sonia Verp, Sandra Eloise Kjeldsen, Alberto De Iaco, Delphine Yvette L Grun

Some of the earliest transcripts produced in fertilized human and mouse oocytes code for DUX, a double homeodomain protein that promotes embryonic genome activation (EGA). Deleting Dux by genome editing at the 1- to 2-cell stage in the mouse impairs EGA an ...

2019

KAP1 facilitates reinstatement of heterochromatin after DNA replication

Didier Trono, Priscilla Turelli, Annamaria Kauzlaric, Julien Léonard Duc, Sandra Eloise Kjeldsen, Benjamin Rauwel, Andrea Coluccio, Suk Min Jang, Julien Paul André Pontis, Geneviève Almouzni

During cell division, maintenance of chromatin features from the parental genome requires their proper establishment on its newly synthetized copy. The loss of epigenetic marks within heterochromatin, typically enriched in repetitive elements, endangers ge ...

2018

Individual retrotransposon integrants are differentially controlled by KZFP/KAP1-dependent histone methylation, DNA methylation and TET-mediated hydroxymethylation in naïve embryonic stem cells

Didier Trono, Priscilla Turelli, Julien Léonard Duc, Sandra Eloise Kjeldsen, Gabriela Ecco, Andrea Coluccio

BACKGROUND: The KZFP/KAP1 (KRAB zinc finger proteins/KRAB-associated protein 1) system plays a central role in repressing transposable elements (TEs) and maintaining parent-of-origin DNA methylation at imprinting control regions (ICRs) during the wave of g ...

2018

Polyphenic trait promotes liver cancer in a model of epigenetic instability

Didier Trono, Sandra Eloise Kjeldsen, Marco Cassano, Alessandra Piersigilli, Suk Min Jang, Evaristo Jose Planet Letschert

Hepatocellular carcinoma (HCC) represents the fifth most common form of cancer worldwide and carries a high mortality rate due to lack of effective treatment. Males are eight times more likely to develop HCC than females, an effect largely driven by sex ho ...

Wiley-Blackwell2017

Transposable Elements and Their KRAB-ZFP Controllers Regulate Gene Expression in Adult Tissues

Didier Trono, Priscilla Turelli, Annamaria Kauzlaric, Julien Léonard Duc, Helen Mary Rowe, Sandra Eloise Kjeldsen, Gabriela Ecco, Marco Cassano, Michaël Imbeault, Andrea Coluccio

KRAB-containing zinc finger proteins (KRAB-ZFPs) are early embryonic controllers of transposable elements (TEs), which they repress with their cofactor KAP1 through histone and DNA methylation, a process thought to result in irreversible silencing. Using a ...

Cell Press - Elsevier2016

The questions you should ask yourself for efficient CRISPR/Cas9 genome editing

Isabelle Barde, Sandra Eloise Kjeldsen

Springer2016

Potential Of Induced Pluripotent Stem Cells For The Treatment Of Crigler-Najjar Liver Disease

Didier Trono, Marc Samuel Friedli, Sandra Eloise Kjeldsen

Elsevier Science Bv2015

Control of mitophagie by microRNAs - A key step of erythropoiesis

Didier Trono, Isabelle Barde, Sonia Verp, Andrea Corsinotti, Jiri Vanicek, Sandra Eloise Kjeldsen, Adamantia Kapopoulou, Ray Marcel Marin Florez, Benjamin Rauwel

Edp Sciences S A2014

De novo DNA methylation of endogenous retroviruses is shaped by KRAB-ZFPs/KAP1 and ESET

Didier Trono, Sonia Verp, Helen Mary Rowe, Marc Samuel Friedli, Sandra Eloise Kjeldsen

Endogenous retroviruses (ERVs) undergo de novo DNA methylation during the first few days of mammalian embryogenesis, although the factors that control the targeting of this process are largely unknown. We asked whether KAP1 (KRAB-associated protein 1) is i ...

Company Of Biologists Ltd2013