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Sebastian Maerkl

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Publications associées (71)

Systematic analysis of low-affinity transcription factor binding site clusters in vitro and in vivo establishes their functional relevance

Sebastian Maerkl, Ivan Istomin, Evan James Olson, Shiyu Cheng, Amir Shahein, Maria Lopez Malo

Binding to binding site clusters has yet to be characterized in depth, and the functional relevance of low-affinity clusters remains uncertain. We characterized transcription factor binding to low-affinity clusters in vitro and found that transcription fac ...

Nature Portfolio2022

A high-throughput microfluidic nanoimmunoassay for detecting anti-SARS-CoV-2 antibodies in serum or ultralow-volume blood samples

Grégoire Michielin, Sebastian Maerkl, Zoe Newell Swank, Hon Ming Andrew Yip

Novel technologies are needed to facilitate large-scale detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) specific antibodies in human blood samples. Such technologies are essential to support seroprevalence studies and vaccine clin ...

National Academy of Sciences2021

OnePot PURE Cell-Free System

Sebastian Maerkl, Barbora Lavickova, Laura Sophie Grasemann

The defined PURE (protein synthesis using recombinant elements) transcription-translation system provides an appealing chassis for cell-free synthetic biology. Unfortunately, commercially available systems are costly, and their tunability is limited. In co ...

2021

A partially self-regenerating synthetic cell

Sebastian Maerkl, Nadanai Laohakunakorn, Barbora Lavickova

Self-regeneration is a fundamental function of all living systems. Here we demonstrate partial molecular self-regeneration in a synthetic cell. By implementing a minimal transcription-translation system within microfluidic reactors, the system is able to r ...

Nature Research2020

Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

Grégoire Michielin, Sebastian Maerkl, Zoe Newell Swank, Nadanai Laohakunakorn, Barbora Lavickova, Amir Shahein, Laura Sophie Grasemann

Cell-free systems offer a promising approach to engineer biology since their open nature allows for well-controlled and characterized reaction conditions. In this review, we discuss the history and recent developments in engineering recombinant and crude e ...

2020

Single-Cell Tracing Dissects Regulation of Maintenance and Inheritance of Transcriptional Reinduction Memory

Sebastian Maerkl, Johannes Becker

Transcriptional memory of gene expression enables adaptation to repeated stimuli across many organisms. However, the regulation and heritability of transcriptional memory in single cells and through divisions remains poorly understood. Here, we combined mi ...

2020

Microfluidic systems for cancer diagnostics

Sebastian Maerkl, Jose Luis Garcia Cordero

Although not employed in the clinic as of yet, microfluidic systems are likely to become a key technology for cancer diagnostics and prognosis. Microfluidic devices have been developed for the analysis of various biomarkers including circulating tumor cell ...

2020

How single-cell immunology is benefiting from microfluidic technologies

Sebastian Maerkl, Fabien Louis Claude Robert Jammes

The immune system is a complex network of specialized cells that work in concert to protect against invading pathogens and tissue damage. Imbalances in this network often result in excessive or absent immune responses leading to allergies, autoimmune disea ...

2020

A Multilayer Microfluidic Platform for the Conduction of Prolonged Cell-Free Gene Expression

Sebastian Maerkl, Zoe Newell Swank

2019

A Simple, Robust, and Low-Cost Method To Produce the PURE Cell Free System

Sebastian Maerkl, Barbora Lavickova

We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in c ...

2019

Cell-free gene-regulatory network engineering with synthetic transcription factors

Sebastian Maerkl, Zoe Newell Swank, Nadanai Laohakunakorn

Gene-regulatory networks are ubiquitous in nature and critical for bottom-up engineering of synthetic networks. Transcriptional repression is a fundamental function that can be tuned at the level of DNA, protein, and cooperative protein-protein interaction ...

National Academy of Sciences2019

Cascaded amplifying circuits enable ultrasensitive cellular sensors for toxic metals

Sebastian Maerkl, Francesca Volpetti, Ekaterina Emilova Petrova

Cell-based biosensors have great potential to detect various toxic and pathogenic contaminants in aqueous environments. However, frequently they cannot meet practical requirements due to insufficient sensing performance. To address this issue, we investiga ...

2019

Microfluidic device for real-time formulation of reagents and their subsequent encapsulation into double emulsions

Sebastian Maerkl, Esther Amstad, Zoe Newell Swank, Jui-Chia Chang

Emulsion drops are often employed as picoliter-sized containers to perform screening assays. These assays usually entail the formation of drops encompassing discrete objects such as cells or microparticles and reagents to study interactions between the dif ...

Nature Research2018

Microfluidic Module for Real-Time Generation of Complex Multimolecule Temporal Concentration Profiles

Sebastian Maerkl, Kristina Pan Woodruff

2018

Microfluidic Transfection for High-Throughput Mammalian Protein Expression

Sebastian Maerkl, Kristina Pan Woodruff

2018

A Microfluidic Biodisplay

Sebastian Maerkl, Francesca Volpetti, Ekaterina Emilova Petrova

Synthetically engineered cells are powerful and potentially useful biosensors, but it remains problematic to deploy such systems due to practical difficulties and biosafety concerns. To overcome these hurdles, we developed a microfluidic device that serves ...

American Chemical Society2017

A system, device and method for multiplexed biomarker diagnostics of ultra-low volume whole blood samples

Sebastian Maerkl, Francesca Volpetti, Francesco Piraino

The invention relates to a microfluidic device for performing digital analysis of a whole blood sample. ...

2017

A High-Throughput Microfluidic Platform for Mammalian Cell Transfection and Culturing

Sebastian Maerkl, Kristina Pan Woodruff

Mammalian synthetic biology could be augmented through the development of high-throughput microfluidic systems that integrate cellular transfection, culturing, and imaging. We created a microfluidic chip that cultures cells and implements 280 independent t ...

Nature Research2016

Mechanically Induced Trapping of Molecular Interactions and Its Applications

Sebastian Maerkl, Jose Luis Garcia Cordero

Measuring binding affinities and association/dissociation rates of molecular interactions is important for a quantitative understanding of cellular mechanisms. Many low-throughput methods have been developed throughout the years to obtain these parameters. ...

Sage Publications Inc2016

Integrating gene synthesis and microfluidic protein analysis for rapid protein engineering

Sebastian Maerkl, Matthew Christopher Blackburn, Ekaterina Emilova Petrova, Bruno Emanuel Ferreira De Sousa Correia

The capability to rapidly design proteins with novel functions will have a significant impact on medicine, biotechnology and synthetic biology. Synthetic genes are becoming a commodity, but integrated approaches have yet to be developed that take full adva ...

Oxford Univ Press2016

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