Publications de Petr Strnad | EPFL Graph Search

Cell-autonomous timing drives the vertebrate segmentation clock's wave pattern

Petr Strnad, Andrew Charles Oates, Daniele Soroldoni, Laurel Ann Rohde, Sundar Ram Naganathan, Arianne Bercowsky Rama

Rhythmic and sequential segmentation of the growing vertebrate body relies on the segmentation clock, a multi-cellular oscillating genetic network. The clock is visible as tissue-level kinematic waves of gene expression that travel through the presomitic m ...

2024

Multiscale light-sheet organoid imaging framework

Raphaël Ortiz, Petr Strnad, Andrea Boni

Live imaging of organoid growth remains a challenge: it requires long-term imaging of several samples simultaneously and dedicated analysis pipelines. Here the authors report an experimental and image processing framework to turn long-term light-sheet imag ...

Nature Portfolio2022

Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells

Pierre Gönczy, Petr Strnad, Isabelle Fluckiger, Fernando Romero Balestra

Centrioles are essential for forming cilia, flagella, and centrosomes and are thus critical for a range of fundamental cellular processes. Despite their importance, the mechanisms governing centriole biogenesis remain incompletely understood. We performed ...

Cell Press - Elsevier2013

Analysis of centriole elimination during C. elegans oogenesis

Graham Knott, Pierre Gönczy, Tamara Mikeladze-Dvali, Petr Strnad, Lukas von Tobel

Centrosomes are the principal microtubule organizing centers (MTOCs) of animal cells and comprise a pair of centrioles surrounded by pericentriolar material (PCM). Centriole number must be carefully regulated, notably to ensure bipolar spindle formation an ...

2012

Spindle positioning in human cells relies on proper centriole formation and on the microcephaly proteins CPAP and STIL

Pierre Gönczy, Debora Keller, Gregor Kohlmaier, Petr Strnad, Fernando Romero Balestra

Patients with MCPH (autosomal recessive primary microcephaly) exhibit impaired brain development, presumably due to the compromised function of neuronal progenitors. Seven MCPH loci have been identified, including one that encodes centrosome protein 4.1 as ...

Company of Biologists2011

Mechanisms of procentriole formation

Pierre Gönczy, Petr Strnad

The centrosome comprises a pair of centrioles and associated pericentriolar material, and it is the principal microtubule-organizing centre of most animal cells. Like the genetic material, the centrosome is duplicated once and only once during the cell cyc ...

2008

Regulated HsSAS-6 levels ensure formation of a single procentriole per centriole during the centrosome duplication cycle

Pierre Gönczy, Petr Strnad

Centrosome duplication involves the formation of a single procentriole next to each centriole, once per cell cycle. The mechanisms governing procentriole formation and those restricting its occurrence to one event per centriole are poorly understood. Here, ...

Cell Press - Elsevier2007