Publications de Johan Auwerx | EPFL Graph Search

Delivery of A Chemically Modified Noncoding RNA Domain Improves Dystrophic Myotube Function

Fast twitch muscle fibers are prone to degradation in skeletal muscle pathologies, such as sarcopenia and muscular dystrophies. We previously showed that the exercise-induced long noncoding RNA CYTOR promotes fast-twitch myogenesis. Here, we identify an in ...

WILEY2025

Porphyran from discolored nori prevents metabolic syndrome through microbiota-bile acid-ceramide pathway

Johan Auwerx, Adrienne Joëlle Laurence Mottis, Alessia Perino, Jean-David Horacio Morel

Nori is a component of the traditional Japanese diet. The Japanese have a low prevalence of obesity and cardiovascular disease worldwide, and components of nori may have disease-preventive effects. Here, we focused on porphyran, which is abundant in nori t ...

2025

Skin health and biological aging

Johan Auwerx

Accumulating evidence indicates that biological aging can be accelerated by environmental exposures, collectively called the ‘exposome’. The skin, as the largest and most exposed organ, can be viewed as a ‘window’ for the deep exploration of the exposome a ...

Springer Science and Business Media LLC2025

Plasmalogen remodeling modulates macrophage response to cytotoxic oxysterols and atherosclerotic plaque vulnerability

Johan Auwerx, Xiaoxu Li

Highlights • LPCAT3 and ELOVL5 coordinate PUFA metabolism in macrophages • Dual LPCAT3/ELOVL5 deletion increases membrane cholesterol-enriched domains • This alteration enhances macrophage sensitivity to cytotoxic oxysterols ...

Elsevier BV2025

A lysosomal surveillance response to stress extends healthspan

Johan Auwerx, Yang Li, Wen Gao, Xiaoxu Li, Amélia Lalou, Tanes Imamura de Lima, Qi Wang, Jia Liu

Lysosomes are cytoplasmic organelles central for the degradation of macromolecules to maintain cellular homoeostasis and health. However, how lysosomal activity can be boosted to counteract ageing and ageing-related diseases remains elusive. Here we reveal ...

Springer Science and Business Media LLC2025

NAD+ boosting increases atherosclerotic plaques and inflammation in Apoe knockout mice

Johan Auwerx

Background and aims: NAD+ (nicotinamide adenine dinucleotide) is a cosubstrate of the sirtuins (SIRT) that are activated upon caloric restriction. Supplementing NAD+ precursors such as nicotinamide riboside (NR) has been reported to extend life span and co ...

2025

The time is now: accounting for time-of-day effects to improve reproducibility and translation of metabolism research

Johan Auwerx

The constant expansion of the field of metabolic research has led to more nuanced and sophisticated understanding of the complex mechanisms that underlie metabolic functions and diseases. Collaborations with scientists of various fields such as neuroscienc ...

NATURE PORTFOLIO2025

Age-associated nicotinamide adenine dinucleotide decline drives CAR-T cell failure

Johan Auwerx

Chimeric antigen receptor (CAR) T cell therapy is one of the most promising cancer treatments. However, different hurdles are limiting its application and efficacy. In this context, how aging influences CAR-T cell outcomes is largely unknown. Here we show ...

Springer Science and Business Media LLC2025

The protein deacetylase SIRT2 exerts metabolic control over adaptive β cell proliferation

Johan Auwerx

Selective and controlled expansion of endogenous β-cells has been pursued as a potential therapy for diabetes. Ideally, such therapies would preserve feedback control of β-cell proliferation to avoid excessive β-cell expansion. Here, we identified a regula ...

2025

Mitochondrial genetics, signalling and stress responses

Johan Auwerx, Jia Liu

Mitochondria are multifaceted organelles with crucial roles in energy generation, cellular signalling and a range of synthesis pathways. The study of mitochondrial biology is complicated by its own small genome, which is matrilineally inherited and not sub ...

NATURE PORTFOLIO2025

ACMSD inhibition corrects fibrosis, inflammation, and DNA damage in MASLD/MASH

Johan Auwerx, Xiaoxu Li, Sandra Rodriguez Lopez, Qi Wang, Jia Liu, Keno Strotjohann

Background & Aims: Recent findings reveal the importance of tryptophan-initiated de novo nicotinamide adenine dinucleotide (NAD+) synthesis in the liver, a process previously considered secondary to biosynthesis from nicotinamide. The enzyme α-amino-β-carb ...

2025

A Multiomic Network Approach to Uncover Disease Modifying Mechanisms of Inborn Errors of Metabolism

Johan Auwerx

For many inborn errors of metabolism (IEM) the understanding of disease mechanisms remains limited, in part explaining their unmet medical needs. The expressivity of IEM disease phenotypes is affected by disease-modifying factors, including rare and common ...

2025

Systems genetics of metabolic health in the BXD mouse genetic reference population

Johan Auwerx, Maroun Bou Sleiman, Wen Gao, Jean-David Horacio Morel, Xiaoxu Li, Alessia De Masi, Amélia Lalou, Jia Liu

Susceptibility to metabolic syndrome (MetS) is dependent on genetics, environment, and gene-by-environment interactions, rendering the study of underlying mechanisms challenging. The majority of experiments in model organisms do not incorporate genetic var ...

2024

High-content phenotypic analysis of a C. elegans recombinant inbred population identifies genetic and molecular regulators of lifespan

Elena Katsyuba, Johan Auwerx, Laurent Mouchiroud, Maroun Bou Sleiman, Yang Li, Gaby El Alam, Wen Gao, Jean-David Horacio Morel, Xiaoxu Li, Amélia Lalou

Lifespan is influenced by complex interactions between genetic and environmental factors. Studying those factors in model organisms of a single genetic background limits their translational value for humans. Here, we mapped lifespan determinants in 85 C. e ...

2024

Cellpose training data and scripts from "Inhibition of CERS1 in aging skeletal muscle exacerbates age-related muscle impairments"

Olivier Burri, Johan Auwerx, Martin Rainer Wohlwend

This Workflow contains all the material necessary to reproduce the results of the QuPath analysis performed in the paper "Inhibition of CERS1 in aging skeletal muscle exacerbates age-related muscle impairments" Inside this workflow and dataset, you will f ...

Zenodo2024

Loss of hepatic Sirt7 accelerates diethylnitrosamine (DEN)-induced formation of hepatocellular carcinoma by impairing DNA damage repair

Kristina Schoonjans, Johan Auwerx

The mammalian sirtuin family (SIRT1-SIRT7) has shown diverse biological roles in the regulation and maintenance of genome stability under genotoxic stress. SIRT7, one of the least studied sirtuin, has been demonstrated to be a key factor for DNA damage res ...

2024

Inhibition of SIRT7 overcomes sorafenib acquired resistance by suppressing ERK1/2 phosphorylation via the DDX3X-mediated NLRP3 inflammasome in hepatocellular carcinoma

Kristina Schoonjans, Johan Auwerx, Pan Xu

Aims: Sirtuin 7 (SIRT7) plays an important role in tumor development, and has been characterized as a potent regulator of cellular stress. However, the effect of SIRT7 on sorafenib acquired resistance remains unclear and a possible anti-tumor mechanism bey ...

2024

Wars1 downregulation in hepatocytes induces mitochondrial stress and disrupts metabolic homeostasis

Kristina Schoonjans, Johan Auwerx, Alessia Perino, Hadrien Charles Edouard Demagny, Xiaoxu Li, Antoine Jalil

Several laboratories, including ours, have employed the Slc25a47tm1c(EUCOMM)Hmgu mouse model to investigate the role of SLC25A47, a hepatocyte-specific mitochondrial carrier, in regulating hepatic metabolism and systemic physiology. In this study, we revea ...

2024

Targeting senescence induced by age or chemotherapy with a polyphenol-rich natural extract improves longevity and healthspan in mice

Johan Auwerx

Accumulating senescent cells within tissues contribute to the progression of aging and age-related diseases. Botanical extracts, rich in phytoconstituents, present a useful resource for discovering therapies that could target senescence and thus improve he ...

2024

Cyclo His-Pro Attenuates Muscle Degeneration in Murine Myopathy Models

Johan Auwerx, Xiaoxu Li, Alessia De Masi, Tanes Imamura de Lima, Keno Strotjohann

Among the inherited myopathies, a group of muscular disorders characterized by structural and metabolic impairments in skeletal muscle, Duchenne muscular dystrophy (DMD) stands out for its devastating progression. DMD pathogenesis is driven by the progress ...

Wiley2024