Publications de Louise Catherine Bryan

Multiplexed single-molecule experiments reveal Cas9 nucleosome invasion dynamics

Louise Catherine Bryan, Beat Fierz, Kristina Makasheva

CELL PRESS2022

A single-molecule multiplexed approach to study Cas9 nucleosomes invasion

Louise Catherine Bryan, Beat Fierz, Kristina Makasheva

SPRINGER2021

Multiplexed Single-Molecule Experiments Reveal Nucleosome Invasion Dynamics of the Cas9 Genome Editor

Louise Catherine Bryan, Beat Fierz, Kristina Makasheva

Single-molecule measurements provide detailed mechanistic insights into molecular processes, for example in genome regulation where DNA access is controlled by nucleosomes and the chromatin machinery. However, real-time single-molecule observations of nucl ...

AMER CHEMICAL SOC2021

KAP1 is an antiparallel dimer with a functional asymmetry

Didier Trono, Davide Demurtas, Matteo Dal Peraro, Louise Catherine Bryan, Beat Fierz, Pierre-Yves Joseph Laurent Helleboid, Alexandra Styliani Kalantzi, Giulia Fonti, Maria Josefina Marcaida Lopez

KAP1 (KRAB domain-associated protein 1) plays a fundamental role in regulating gene expression in mammalian cells by recruiting different transcription factors and altering the chromatin state. In doing so, KAP1 acts both as a platform for macromolecular i ...

Life Science Alliance, LLC2019

Chromatin Remodeling Induced by the Invasion of Yeast Pioneer Transcription Factor Rap1 Revealed by Single-Molecule FRET

Louise Catherine Bryan, Maxime Nicolas Mivelaz, Beat Fierz, Iuliia Boichenko, Anne-Marinette Thi Phuong Hanh Cao

CELL PRESS2019

Single-molecule FRET reveals multiscale chromatin dynamics modulated by HP1α

Louise Catherine Bryan, Beat Fierz, Sinan Kilic, Iuliia Boichenko

The dynamic architecture of chromatin fibers, a key determinant of genome regulation, is poorly understood. Here, we employ multimodal single-molecule Förster resonance energy transfer studies to reveal structural states and their interconversion kinetics ...

Nature Research2018

Deciphering the chromatin binding mechanisms of human HP1 variants at the single-molecule level

Louise Catherine Bryan

In eukaryotic cells, DNA is tightly packed in the form chromatin. The basic structure of chromatin is a nucleosome composed of 147 bp DNA wrapped around eight histone proteins; two copies of H2A, H2B, H3 and H4. These histone proteins are decorated with pa ...

EPFL2018

Single-molecule kinetic analysis of HP1-chromatin binding reveals a dynamic network of histone modification and DNA interactions

Vassily Hatzimanikatis, Louise Catherine Bryan, Georg Fantner, Pascal Damian Odermatt, Oliver Hantschel, Sandrine Madeleine Suzanne Georgeon, Beat Fierz, Sinan Kilic, Carolin Christine Lechner, Andreas Linus Bachmann, Daniel Robert Weilandt

Chromatin recruitment of effector proteins involved in gene regulation depends on multivalent interaction with histone post-translational modifications (PTMs) and structural features of the chromatin fiber. Due to the complex interactions involved, it is c ...

Oxford University Press (OUP)2017

Physicochemical Characterization of Nebulized Superparamagnetic Iron Oxide Nanoparticles (SPIONs)

Paul Bowen, Géraldine Sylvie Coullerez, Louise Catherine Bryan, Guillaume Suarez

Background: Aerosol-mediated delivery of nano-based therapeutics to the lung has emerged as a promising alternative for treatment and prevention of lung diseases. Superparamagnetic iron oxide nanoparticles (SPIONs) have attracted significant attention for ...

Mary Ann Liebert, Inc2015

Multivalency governs HP1α association dynamics with the silent chromatin state

Louise Catherine Bryan, Beat Fierz, Sinan Kilic, Andreas Linus Bachmann

Multivalent interactions between effector proteins and histone post-translational modifications are an elementary mechanism of dynamic chromatin signalling. Here we elucidate the mechanism how heterochromatin protein 1α (HP1α), a multivalent effector, is e ...

Nature Research2015

Heterochromatin Assembly and Dynamics on the Single Molecule Level

Louise Catherine Bryan, Beat Fierz, Sinan Kilic

Cell Press2015

Molecular and Chemical Mechanism in Epigenetics – Swiss Summer School 2015 July 12–17, 2015, Hotel Kurhaus, Arolla, Switzerland

Louise Catherine Bryan, Beat Fierz, Andreas Linus Bachmann

Swiss Chemical Soc2015