Publications de Grazvydas Lukinavicius

Fluorogenic Probes for Multicolor Imaging in Living Cells

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius, Olivier Vincent Sallin, Keitaro Umezawa

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 nm and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes and SNAP-tag ar ...

American Chemical Society2016

SiR–Hoechst is a far-red DNA stain for live-cell nanoscopy

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius, Alberto Schena

Cell-permeable DNA stains are popular markers in live-cell imaging. Currently used DNA stains for live-cell imaging are either toxic, require illumination with blue light or are not compatible with super-resolution microscopy, thereby limiting their utilit ...

Nature Research2015

Reduced Dyes Enhance Single-Molecule Localization Density for Live Superresolution Imaging

Luc Reymond, Grazvydas Lukinavicius, Suliana Manley, Aleksandr Benke, Lina Carlini

Cell-permeable rhodamine dyes are reductively quenched by NaBH4 into a non-fluorescent leuco-rhodamine form. Quenching is reversible, and their fluorescence is recovered when the dyes are oxidized. In living cells, oxidation occurs spontaneously, and can r ...

Wiley-V C H Verlag Gmbh2014

Selective Chemical Crosslinking Reveals a Cep57-Cep63-Cep152 Centrosomal Complex

Kai Johnsson, Luc Reymond, Pierre Gönczy, Grazvydas Lukinavicius, Keitaro Umezawa

The centrosome functions as the main microtubule-organizing center of animal cells and is crucial for several fundamental cellular processes [1]. Abnormalities in centrosome number and composition correlate with tumor progression [2, 3] and other diseases ...

Elsevier2013

Cell permeable, fluorescent dye

Kai Johnsson, Grazvydas Lukinavicius, Keitaro Umezawa

The invention pertains to a near-infrared fluorescent dye that is cell permeable and can be attached to selected proteins in living cells. The dye has the general formula (I) or its or its corresponding spirolactone (II) wherein Y is chosen from the group ...

2013

Substrates for Improved Live-Cell Fluorescence Labeling of SNAP-tag

Kai Johnsson, Luc Reymond, Grazvydas Lukinavicius

The SNAP-tag labeling technology provides a simple, robust, and versatile approach to the imaging of fusion proteins for a wide range of experimental applications. Owing to the specific and covalent nature of the labeling reaction, SNAP-tag is well suited ...

Bentham Science Publ Ltd2013

Visualizing Biochemical Activities in Living Cells through Chemistry

Kai Johnsson, Luc Reymond, Matthias Albert Brun, Karolina Bojkowska, Birgit Mollwitz, Grazvydas Lukinavicius, Anastasiya Masharina, Alberto Schena, Keitaro Umezawa, Rudolf Griss

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over ...

2011

Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

Iwan Märki, Theo Lasser, Kai Johnsson, Claudio Dellagiacoma, Stefan Geissbühler, Sambashiva Banala, Grazvydas Lukinavicius, Noelia Laura Bocchio

We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged mi ...

2010