Publications de Niccolo Piacentini

Toward a Physiologically Relevant 3D Helicoidal-Oriented Cardiac Model: Simultaneous Application of Mechanical Stimulation and Surface Topography

Philippe Renaud, Niccolo Piacentini, Fatemeh Navaee

Myocardium consists of cardiac cells that interact with their environment through physical, biochemical, and electrical stimulations. The physiology, function, and metabolism of cardiac tissue are affected by this dynamic structure. Within the myocardium, ...

MDPI2023

Additive manufacturing of hierarchical injectable scaffolds for tissue engineering

Philippe Renaud, Thomas Michael Braschler, Niccolo Piacentini

We present a 3D-printing technology allowing free-form fabrication of centimetre-scale injectable structures for minimally invasive delivery. They result from the combination of 3D printing onto a cryogenic substrate and optimisation of carboxymethylcellul ...

2018

Multimaterial Nanoporous Membranes Shaped through High Aspect-Ratio Sacrificial Silicon Nanostructures

Philippe Renaud, Arnaud Bertsch, Stefano Silvio Giovanni Varricchio, Niccolo Piacentini

We present an innovative fabrication method for solid-state nanoporous membranes based on the casting of sacrificial silicon nanostructures. The process allows the individual definition of geometry and placement of each nanopore through e-beam lithography ...

Amer Chemical Soc2017

Fabrication of nanoporous membranes through silicon templates : two different approaches

Philippe Renaud, Arnaud Bertsch, Stefano Silvio Giovanni Varricchio, Niccolo Piacentini

We present here a novel approach to fabricate nanoporous membranes based on the use of sacrificial template structures to individually define pore parameters such as geometry, shape, and placement. Based on this rationale two different nano-fabrication met ...

2016

Design And Fabrication Of A Well-set, Cost-effective Device For Mechanical And Biochemical Stimulation In Cardiac Tissue Engineering Applications

Philippe Renaud, Niccolo Piacentini, Fatemeh Navaee

2016

Intercellular ultrafast Ca2+ wave in vascular smooth muscle cells: numerical and experimental study

Jean-Jacques Meister, Niccolo Piacentini, Jairo Camilo Quijano Perez, Franck Claude Raynaud

Vascular smooth muscle cells exhibit intercellular Ca2+ waves in response to local mechanical or KCl stimulation. Recently, a new type of intercellular Ca2+ wave was observed in vitro in a linear arrangement of smooth muscle cells. The intercellular wave w ...

Nature Research2016

Evaluating Tension in Actomyosin Bundles at the Cell Periphery

Jean-Jacques Meister, Niccolo Piacentini, Benoît Vianay, Céline Jeanne Labouesse

Cell Press2014

Ultra-soft cantilevers and 3-D micro-patterned substrates for contractile bundle tension measurement in living cells

Jean-Jacques Meister, Alexander Verkhovskiy, Chiara Gabella, Niccolo Piacentini, Benoît Vianay

Actin-myosin microfilament bundles or stress-fibers are the principal tension-generating structures in the cell. Their mechanical properties are critical for cell shape, motion, and interaction with other cells and extracellular matrix, but were so far dif ...

Royal Society of Chemistry2014

Contact Angle at the Leading Edge Controls Cell Protrusion Rate

László Forró, Jean-Jacques Meister, Alexander Verkhovskiy, Sylvia Jeney, Chiara Gabella, Elena Bertseva, Niccolo Piacentini, Alicia Carine Bornert

Plasma membrane tension and the pressure generated by actin polymerization are two antagonistic forces believed to define the protrusion rate at the leading edge of migrating cells [1-5]. Quantitatively, resistance to actin protrusion is a product of membr ...

Elsevier2014

Multiple-frequency impedance measurements in continuous flow for automated evaluation of yeast cell lysis

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

This paper reports on an electrical lysis device integrating multiple-frequency impedance measurements for automated evaluation of the lysis efficiency and counting of the lysed cells. Conventional techniques use chemical reagents to dissolve the cell memb ...

Elsevier2012

Separation of platelets from other blood cells in continuous-flow by dielectrophoresis field-flow-fractionation

Philippe Renaud, Raphaël Tornay, Niccolo Piacentini

We present a microfluidic device capable of separating platelets from other blood cells in continuous flow using dielectrophoresis field-flow-fractionation. The use of hydrodynamic focusing in combination with the application of a dielectrophoretic force a ...

American Institute of Physics2011

Cell viability assessment by flow cytometry using yeast as cell model

Philippe Renaud, Raphaël Tornay, Nina Buffi, Niccolo Piacentini

This paper reports the new combination of cell sorting and counting capabilities on a single device. Most state-of-the-art devices combining these technologies use optical techniques requiring complicated experimental setups and labeled samples. The use of ...

2011

Multiple-frequency Impedance Measurements in Continuous Flow for the Evaluation of Electrical Lysis of Yeast Cells

Philippe Renaud, Niccolo Piacentini, Willyan Hasenkamp Carreira

his paper reports on an electrical lysis device with integrated cell counting and evaluation of the lysis efficiency by multiplefrequency impedance measurements. Conventional techniques use chemical reagents to lyse cells, causing unwanted interference wit ...

Elsevier2010

Continuous-flow electrical lysis device with integrated control by dielectrophoretic cell sorting

Philippe Renaud, Nicolas Demierre, Thomas Michael Braschler, Niccolo Piacentini

We present a device capable of electrical cell lysis and evaluation of lysis efficiency in continuous flow using dielectrophoretic cell sorting. We use a combination of AC electrical fields and so-called liquid electrodes to avoid bubble creation at the el ...

Royal Society of Chemistry2010