Recherche

Afficher tous les résultats pour

Accueil

Personne

Melanie Blokesch

À propos
Confidentialité
Mentions légales

Graph Chatbot

Publications associées (63)

Ecological implications of gene regulation by TfoX and TfoY among diverse Vibrio species

Noémie Matthey, Melanie Blokesch, Esther Jeanne Collas, Candice Stoudmann, Lisa Christina Metzger

Bacteria of the genus Vibrio are common members of aquatic environments where they compete with other prokaryotes and defend themselves against grazing predators. A macromolecular protein complex called the type VI secretion system (T6SS) is used for both ...

2019

DNA-uptake pili of Vibrio cholerae are required for chitin colonization and capable of kin recognition via sequence-specific self-interaction

Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann, David William Adams

How bacteria colonize surfaces and how they distinguish the individuals around them are fundamental biological questions. Type IV pili are a widespread and multipurpose class of cell surface polymers. Here we directly visualize the DNA-uptake pilus of Vibr ...

2019

Cellular Microbiology Interview - Dr. Melanie Blokesch

Melanie Blokesch

2019

Neighbor predation linked to natural competence fosters the transfer of large genomic regions in Vibrio cholerae

Christian Iseli, Nicolas Jean Philippe Guex, Noémie Matthey, Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann

Natural competence for transformation is a primary mode of horizontal gene transfer. Competent bacteria are able to absorb free DNA from their surroundings and exchange this DNA against pieces of their own genome when sufficiently homologous. However, the ...

2019

Mechanisms of DNA Uptake by Naturally Competent Bacteria

Melanie Blokesch

Transformation is a widespread mechanism of horizontal gene transfer in bacteria. DNA uptake to the periplasmic compartment requires a DNA-uptake pilus and the DNA-binding protein ComEA. In the gram-negative bacteria, DNA is first pulled toward the outer m ...

2019

The type IV pilus protein PilU functions as a PilT-dependent retraction ATPase

Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann, David William Adams, Jorge André Matias Pereira

Type IV pili are dynamic cell surface appendages found throughout the bacteria. The ability of these structures to undergo repetitive cycles of extension and retraction underpins their crucial roles in adhesion, motility and natural competence for transfor ...

2019

Eco-Evolutionary Dynamics Linked to Horizontal Gene Transfer in Vibrios

Melanie Blokesch, Frédérique Marie Jeanne Le Roux

Vibrio is a genus of ubiquitous heterotrophic bacteria found in aquatic environments. Although they are a small percentage of the bacteria in these environments, vibrios can predominate during blooms. Vibrios also play important roles in the degradation of ...

2018

Molecular insights into Vibrio cholerae’s intra-amoebal host-pathogen interactions

Graham Knott, Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann, Charles Ulric V Van der Henst, Tiziana Scrignari, Catherine Maclachlan, Natalia Carolina Drebes Dorr, Audrey Sophie Vanhove

Vibrio cholerae, which causes the diarrheal disease cholera, is a species of bacteria commonlyfound in aquatic habitats. Within such environments, the bacterium must defend itselfagainst predatory protozoan grazers. Amoebae are prominent grazers, withAcant ...

Nature Research2018

Long-Read-Based Genome Sequences of Pandemic and Environmental Vibrio cholerae Strains

Noémie Matthey, Melanie Blokesch, Natalia Carolina Drebes Dorr

The bacterium Vibrio cholerae exhibits two distinct lifestyles, one as an aquatic bacterium and the other as the etiological agent of the pandemic human disease cholera. Here, we report closed genome sequences of two seventh pandemic V. cholerae O1 El Tor ...

2018

QstR-dependent regulation of natural competence and type VI secretion in Vibrio cholerae

Melanie Blokesch, Candice Stoudmann, Sandrine Stutzmann, Milena Jadwiga Jaskolska

During growth on chitinous surfaces in its natural aquatic environment Vibrio cholerae develops natural competence for transformation and kills neighboring non-immune bacteria using a type VI secretion system (T6SS). Activation of these two phenotypes requ ...

Oxford University Press2018

Bacterial type VI secretion system facilitates niche domination

Melanie Blokesch, Natalia Carolina Drebes Dorr

Over the last few decades humanity has experienced a series of tremendous technological advances, especially in the realm of basic science. A plethora of new approaches has allowed us to appreciate life in previously unimaginable detail, prompting the real ...

2018

Interbacterial predation as a strategy for DNA acquisition in naturally competent bacteria

Melanie Blokesch

Natural competence enables bacteria to take up exogenous DNA. The evolutionary function of natural competence remains controversial, as imported DNA can act as a source of substrates or can be integrated into the genome. Exogenous homologous DNA can also b ...

Nature Publishing Group2017

Modeling Key Drivers of Cholera Transmission Dynamics Provides New Perspectives for Parasitology

Andrea Rinaldo, Enrico Bertuzzo, Melanie Blokesch, Lorenzo Mari, Marino Gatto

Hydroclimatological and anthropogenic factors are key drivers of waterborne disease transmission. Information on human settlements and host mobility on waterways along which pathogens and hosts disperse, and relevant hydroclimatological processes, can be a ...

2017

In and out—contribution of natural transformation to the shuffling of large genomic regions

Melanie Blokesch

Naturally competent bacteria can pull free DNA from their surroundings. This incoming DNA can serve various purposes, ranging from acting as a source of nutrients or DNA stretches for repair to the acquisition of novel genetic information. The latter proce ...

Elsevier2017

Regulation of competence-mediated horizontal gene transfer in the natural habitat of Vibrio cholerae

Melanie Blokesch, Lisa Christina Metzger

The human pathogen Vibrio cholerae is an autochthonous inhabitant of aquatic environments where it often interacts with zooplankton and their chitinous molts. Chitin induces natural competence for transformation in V. cholerae, a key mode of horizontal gen ...

Elsevier2016

Natural competence for transformation

Melanie Blokesch

While most molecular biologists are familiar with the artificial transformation of bacteria in the context of laboratory cloning experiments, natural competence for transformation refers to a specific physiological state in which prokaryotes are able to ta ...

2016

Circulation of a Quorum-Sensing-Impaired Variant of Vibrio cholerae Strain C6706 Masks Important Phenotypes

Melanie Blokesch, Sandrine Stutzmann

Vibrio cholerae, the causative agent of cholera, is a model organism for studying virulence regulation, biofilm formation, horizontal gene transfer, and the cell-to-cell communication known as quorum sensing (QS). As in any research field, discrepancies be ...

Amer Soc Microbiology2016

Independent Regulation of Type VI Secretion in Vibrio cholerae by TfoX and TfoY

Noémie Matthey, Melanie Blokesch, Sandrine Stutzmann, Charles Ulric V Van der Henst, Tiziana Scrignari, Lisa Christina Metzger

Type VI secretion systems (T6SSs) are nanomachines used for interbacterial killing and intoxication of eukaryotes. Although Vibrio cholerae is a model organism for structural studies on T6SSs, the underlying regulatory network is less understood. A recent ...

Elsevier2016

The DNA-Uptake Process of Naturally Competent Vibrio cholerae

Noémie Matthey, Melanie Blokesch

The sophisticated DNA-uptake machinery used during natural transformation is still poorly characterized, especially in Gram-negative bacteria where the transforming DNA has to cross two membranes as well as the peptidoglycan layer before entering into the ...

Elsevier2016

Competence-induced type VI secretion might foster intestinal colonization by Vibrio cholerae

Melanie Blokesch

The human pathogen Vibrio cholerae exhibits two distinct lifestyles: one in the aquatic environment where it often associates with chitinous surfaces and the other as the causative agent of the disease cholera. While much of the research on V. cholerae has ...

Wiley-Blackwell2015

Page 2 sur 4