Publications de Isabelle Fluckiger

Identification of Chlamydomonas Central Core Centriolar Proteins Reveals a Role for Human WDR90 in Ciliogenesis

Pierre Gönczy, Coralie Busso, Virginie Hamel, Florence Armand, Isabelle Fluckiger, Romain Hamelin, Paul Guichard

Centrioles are evolutionarily conserved macromolecular structures that are fundamental to form cilia, flagella, and centrosomes. Centrioles are 9-fold symmetrical microtubule-based cylindrical barrels comprising three regions that can be clearly distinguis ...

2017

Cell-free reconstitution reveals centriole cartwheel assembly mechanisms

Henning Paul-Julius Stahlberg, Pierre Gönczy, Mircea Ioan Iacovache, Virginie Hamel, Isabelle Fluckiger, Paul Guichard, Veronika Nemcíková Villímová, Niccolo Banterle

How cellular organelles assemble is a fundamental question in biology. The centriole organelle organizes around a nine-foldsymmetrical cartwheel structure typically 100 nm high comprising a stack of rings that each accommodates nine homodimers of SAS-6 pro ...

Springer Nature2017

Correlative multicolor 3D SIM and STORM microscopy

Mathias Fournier, Pierre Gönczy, Virginie Hamel, Arne Seitz, Isabelle Fluckiger, Paul Guichard, Romain Guiet

Within the last decade, super-resolution methods that surpass the diffraction limit of light microscopy have provided invaluable insight into a variety of biological questions. Each of these approaches has inherent advantages and limitations, such that the ...

Optical Soc Amer2014

Caenorhabditis elegans centriolar protein SAS-6 forms a spiral that is consistent with imparting a ninefold symmetry

Pierre Gönczy, Virginie Hamel, Isabelle Fluckiger, Paul Guichard, Georgios Hatzopoulos

Centrioles are evolutionary conserved organelles that give rise to cilia and flagella as well as centrosomes. Centrioles display a characteristic ninefold symmetry imposed by the spindle assembly abnormal protein 6 (SAS-6) family. SAS-6 from Chlamydomonas ...

2013

Discovering Regulators of Centriole Biogenesis through siRNA-Based Functional Genomics in Human Cells

Pierre Gönczy, Petr Strnad, Isabelle Fluckiger, Fernando Romero Balestra

Centrioles are essential for forming cilia, flagella, and centrosomes and are thus critical for a range of fundamental cellular processes. Despite their importance, the mechanisms governing centriole biogenesis remain incompletely understood. We performed ...

Cell Press - Elsevier2013

Native Architecture of the Centriole Proximal Region Reveals Features Underlying Its 9-Fold Radial Symmetry

Aitana Neves da Silva, Pierre Gönczy, Davide Demurtas, Isabelle Fluckiger, Paul Guichard, Norbert Majubu

Centrioles are cylindrical microtubule-based structures whose assembly is critical for the formation of cilia, flagella, and centrosomes. The centriole proximal region harbors a cartwheel that dictates the 9-fold symmetry of centrioles. Although the cartwh ...

2013

Phosphorylation of SAS-6 by ZYG-1 is critical for centriole formation in C. elegans embryos

Pierre Gönczy, Coralie Busso, Isabelle Fluckiger

Despite being essential for proper cell division, the mechanisms governing centrosome duplication are incompletely understood and represent an important open question in cell biology. Formation of a new centriole next to each existing one is critical for c ...

Cell Press - Elsevier2009