Publications de Santiago Harald Andany

A hermetically closed sample chamber enables time-lapse nano-characterization of pathogenic microorganisms in vitro

John McKinney, Santiago Harald Andany, Georg Fantner, Navid Asmari Saadabad

Pathogenic microorganisms, such as pathogenic mycobacteria, pose a global health burden. Studying these organisms is crucial for gaining detailed knowledge about the pathogens and the diseases they cause. To handle pathogenic organisms, specific biosafety ...

ROYAL SOC CHEMISTRY2025

Enhanced feedback performance in off-resonance AFM modes through pulse train sampling

Santiago Harald Andany, Georg Fantner, Mustafa Kangül, Navid Asmari Saadabad, Marcos Penedo Garcia

Dynamic atomic force microscopy (AFM) modes that operate at frequencies far away from the resonance frequency of the cantilever (off-resonance tapping (ORT) modes) can provide high-resolution imaging of a wide range of sample types, including biological sa ...

Beilstein-Institut2024

Advances in High-Speed, Multiparametric Atomic Force Microscopy

Santiago Harald Andany

After decades of technological advancements, high-speed atomic force microscopy (HS-AFM) has emerged as a powerful technique for visualizing dynamic processes. At the nanoscale, the AFM provides valuable insights into the sample by sensing minute interacti ...

EPFL2024

High-Speed Atomic Force Microscopy Imaging of DNA Three-Point-Star Motif Self Assembly Using Photothermal Off-Resonance Tapping

Santiago Harald Andany, Georg Fantner, Bahareh Ghadiani, Mustafa Kangül, Maartje Martina Cornelia Bastings, Cem Tekin, Veronika Cencen, Marcos Penedo Garcia

High-speed atomic force microscopy (HS-AFM) is a popular molecular imaging technique for visualizing single-molecule biological processes in real-time due to its ability to image under physiological conditions in liquid environments. The photothermal off-r ...

Journal Of Visualized Experiments2024

A polymer-semiconductor-ceramic cantilever for high-sensitivity fluidcompatible microelectromechanical systems

Oliver Peric, Santiago Harald Andany, Georg Fantner, Dirk Grundler, Matthias Neuenschwander, Nahid Hosseini, Marcos Penedo Garcia

Active microelectromechanical systems (MEMS) with integrated electronic sensing and actuation can provide fast and sensitive measurements of force, acceleration and biological analytes. Strain sensors integrated onto MEMS cantilevers are widely used to tra ...

Springer Science and Business Media LLC2024

A high-bandwidth voltage amplifier for driving piezoelectric actuators in high-speed atomic force microscopy

Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Mustafa Kangül

High-speed atomic force microscopy (HS-AFM) is a technique capable of revealing the dynamics of biomolecules and living organisms at the nanoscale with a remarkable temporal resolution. The phase delay in the feedback loop dictates the achievable speed of ...

AIP Publishing2023

Data-Driven Feedforward Hysteresis Compensation with Genetic Algorithm for Atomic Force Microscope

Alireza Karimi, Santiago Harald Andany, Georg Fantner, Mustafa Kangül, Navid Asmari Saadabad

Nonlinear dynamics of piezo actuators such as hysteresis, distort the Atomic Force Microscopy (AFM) images as they adversely affect the accuracy of the nano-positioning setup. To compensate for the effects of hysteresis on lateral scanner actuators of AFM, ...

IEEE2022

Kinetic and structural roles for the surface in guiding SAS-6 self-assembly to direct centriole architecture

Pierre Gönczy, Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Georgios Hatzopoulos, Niccolo Banterle, Charlène Cécile Geneviève Brillard

Discovering mechanisms governing organelle assembly is a fundamental pursuit in biology. The centriole is an evolutionarily conserved organelle with a signature 9-fold symmetrical chiral arrangement of microtubules imparted onto the cilium it templates. Th ...

Nature Portfolio2021

Self-Actuated Polymer-Based Cantilevers With Sharp Silicon Tips For High-Speed Atomic Force Microscopy

Santiago Harald Andany, Georg Fantner, Matthias Neuenschwander, Nahid Hosseini

High-speed atomic force microscopy has thus far largely been limited to the use of small cantilevers in liquid, but the technique would greatly profit areas of nanotechnology that require the use of larger probes in air. We present polymer-based cantilever ...

IEEE2021

Tuning SAS-6 architecture with monobodies impairs distinct steps of centriole assembly

Tatiana Favez, Pierre Gönczy, Virginie Hamel, Santiago Harald Andany, Georg Fantner, Oliver Hantschel, Georgios Hatzopoulos, Niccolo Banterle

Centrioles are evolutionarily conserved multi-protein organelles essential for forming cilia and centrosomes. Centriole biogenesis begins with self-assembly of SAS-6 proteins into 9-fold symmetrical ring polymers, which then stack into a cartwheel that sca ...

Nature Research2021

An atomic force microscope integrated with a helium ion microscope for correlative nanoscale characterization

Santiago Harald Andany, Georg Fantner, Charlène Cécile Geneviève Brillard

In this work, we report on the integration of an atomic force microscope (AFM) into a helium ion microscope (HIM). The HIM is a powerful instrument, capable of imaging and machining of nanoscale structures with sub-nanometer resolution, while the AFM is a ...

BEILSTEIN-INSTITUT2020

Integration of sharp silicon nitride tips into high-speed SU8 cantilevers in a batch fabrication process

Oliver Peric, Santiago Harald Andany, Georg Fantner, Jonathan David Adams, Matthias Neuenschwander, Nahid Hosseini

Employing polymer cantilevers has shown to outperform using their silicon or silicon nitride analogues concerning the imaging speed of atomic force microscopy (AFM) in tapping mode (intermittent contact mode with amplitude modulation) by up to one order of ...

Beilstein Institut2019

High-speed photothermal off-resonance atomic force microscopy reveals assembly routes of centriolar scaffold protein SAS-6

Pierre Gönczy, Santiago Harald Andany, Georg Fantner, Adrian Pascal Nievergelt, Niccolo Banterle

The self-assembly of protein complexes is at the core of many fundamental biological processes1, ranging from the polymerization of cytoskeletal elements, such as microtubules2, to viral capsid formation and organelle assembly3. To reach a comprehensive un ...

2018

Digitally controlled analog proportional-integral-derivative (PID) controller for high-speed scanning probe microscopy

Santiago Harald Andany, Georg Fantner, Maja Dukic Pjanic, Nahid Hosseini, Adrian Pascal Nievergelt, Chen Yang

Nearly all scanning probe microscopes (SPMs) contain a feedback controller, which is used to move the scanner in the direction of the z-axis in order to maintain a constant setpoint based on the tip-sample interaction. The most frequently used feedback con ...

2017

Components for high-speed atomic force microscopy optimized for low phase-lag

Santiago Harald Andany, Georg Fantner, Jonathan David Adams, Adrian Pascal Nievergelt, Mélanie Thérèse Marie Hannebelle

IEEE2017