Publications de Robert Meissner

Device for high- throughput sample slicing and characterization

The invention relates to devices and systems for use in the slicing, separation and possible disposal of slices of a sample, such as a bodily tissue sample. The invention relates to a multi-blade chopper and a guide track comprising knobs along its surface ...

2017

Device and method for testing a material response to a compound

Philippe Renaud, Robert Meissner

Device for testing a material response, such as a tissue response, to a compound; said device comprising material impedance measuring means which are adapted to measure the material impedance at the compound action site. ...

2015

Impedance spectroscopy for in vitro toxicology

Robert Meissner

The impedance of biological material changes with frequency, a phenomenon that has been discovered more than 100 years ago. It is due to the fact that the cell membrane acts as a capacitor which filters out currents at low frequency and lets them pass at h ...

EPFL2013

Label-Free Recognition of Drug Resistance via Impedimetric Screening of Breast Cancer Cells

Philippe Renaud, Arnaud Bertsch, Robert Meissner, Bilge Eker

We present a novel study on label-free recognition and distinction of drug resistant breast cancer cells (MCF-7 DOX) from their parental cells (MCF-7 WT) via impedimetric measurements. Drug resistant cells exhibited significant differences in their dielect ...

Public Library of Science2013

A microfluidic-based frequency-multiplexing impedance sensor (FMIS)

Philippe Renaud, Arnaud Bertsch, Pierre Joris, Robert Meissner, Bilge Eker

We present a novel technology for the simultaneous and simple impedimetric screening of multiple microfluidic channels with only one electrode pair. We have exploited the frequency dimension to distinguish between up to three channels. Each ‘sub-sensor’ po ...

2012

Continuous and label-free toxicity screening of human hepatocytes on chip reveals frequency-dependant impedance profiles

Philippe Renaud, Robert Meissner, Bilge Eker

We present an electrical impedance based biosensor on a microfluidic cell culture platform that enables label-free and re- al-time investigation of drug toxicity-induced effects on hepatocytes. The temporal evolution of the impedance at different frequenci ...

2011

Co-pathological states of tau proteins in a 3D micropatterned neural cell culture

Philippe Renaud, Anja Kunze, Robert Meissner, Serena Brando

In vitro studies of Alzheimer’s disease are based on homogenously seeded neural cells. These studies reveal molecular pathway mechanism related to neurite degeneration, but they cannot analyze interactions between diseased and healthy cells. Furthermore, t ...

2011

Distinguishing drug-induced minor morphological changes from major cellular damage via label-free impedimetric toxicity screening

Philippe Renaud, Arnaud Bertsch, Sri Harsha Kasi Raj, Robert Meissner, Bilge Eker

We present a novel perfusion-based microfluidic platform for label-free drug toxicity screening which can single out non-lethal morphological changes from cellular death using electrical impedance spectroscopy. Minor cellular changes such as cell-cell cont ...

2011

Co-Pathological Connected Primary Neurons in a Microfluidic Device for Alzheimer Studies

Philippe Renaud, Anja Kunze, Robert Meissner, Serena Brando

This communication presents a novel experi- mental model for Alzheimer studies, where connected pri- mary neurons were set into subtend, co-pathological states. Cortical neurons were cultured in two separated cell com- partments in a microfluidic device. A ...

Wiley-Blackwell2011

A Ca2+ ion selective electrode biosensor in microfluidics to monitor hepatocyte’s activities

Philippe Renaud, Harald Van Lintel, Robert Meissner

Ca2+ plays a crucial role in cell communication. For example, periodical changes in Ca2+ concentration act as cell sig- nals. Intracellular Ca2+ signals have been measured by Ca2+ sensitive fluorescent dye [1]. In this study, extracellular Ca2+ variation w ...

2010

Neurite guidance through 3D hydrogel layers in a microfluidic environment

Philippe Renaud, Anja Kunze, Robert Meissner

Neuronal cell culture models in vitro are often restricted to 2D surfaces. Engineering the complexity of the neuronal microenvironment in microfluidic systems can help to generate more tissue like cultures. We have developed a new neuronal cell culturing s ...

2010