Publications de Matteo Cornaglia

Automated phenotyping of Caenorhabditis elegans embryos with a high-throughput-screening microfluidic platform

Martinus Gijs, Matteo Cornaglia, Hüseyin Baris Atakan, Tunç Alkanat, Raphaël Etienne Jean Trouillon

The nematode Caenorhabditis elegans has been extensively used as a model multicellular organism to study the influence of osmotic stress conditions and the toxicity of chemical compounds on developmental and motility-associated phenotypes. However, the sev ...

2020

Anin vivomicrofluidic study of bacterial transit inC. elegansnematodes

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Hüseyin Baris Atakan, Vittorio Viri

Caenorhabditis elegans(C. elegans) constitutes an important model organism for use in nutrition and aging studies. We report a novel method for studying the dynamics ofEscherichia coli(E. coli) bacterial transit through the worms' intestine. A microfluidic ...

2020

Microfluidic system for Caenorhabditis elegans culture and oxygen consumption rate measurements

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Roger Philippe Krenger

Mitochondrial respiration is a key signature for the assessment of mitochondrial functioning and mitochondrial dysfunction is related to many diseases including metabolic syndrome and aging-associated conditions. Here, we present a microfluidic Caenorhabdi ...

ROYAL SOC CHEMISTRY2020

Automated Platform for Long-Term Culture and High-Content Phenotyping of Single C. elegans Worms

Martinus Gijs, Elena Katsyuba, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang

The nematode Caenorhabditis elegans is a suitable model organism in drug screening. Traditionally worms are grown on agar plates, posing many challenges for long-term culture and phenotyping of animals under identical conditions. Microfluidics allows for ' ...

Nature Research2019

The Detection of Early Epigenetic Inheritance of Mitochondrial Stress in C. Elegans with a Microfluidic Phenotyping Platform

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Hüseyin Baris Atakan

Fluctuations and deterioration in environmental conditions potentially have a phenotypic impact that extends over generations. Transgenerational epigenetics is the defined term for such intergenerational transient inheritance without an alteration in the D ...

Nature Research2019

Automated High-Content Phenotyping Of The Nematode C. Elegans At Single Animal Resolution With A Microfluidic Platform

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan, Rongrong Xiang

In this work, we present a novel microfluidic approach to culture the nematode C. elegans from the onset of embryogenesis to the adult stage at single animal resolution. Our device allows high-throughput experimentation, high-resolution imaging, thereby pr ...

IEEE2019

Automated high-content phenotyping from the first larval stage till the onset of adulthood of the nematode Caenorhabditis elegans

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan

The nematode Caenorhabditis elegans is increasingly used as a model for human biology. However, in vivo culturing platforms for C. elegans allowing high-content phenotyping during their life cycle in an automated fashion are lacking so far. Here, a multipl ...

ROYAL SOC CHEMISTRY2019

Multimodal imaging and high-throughput image-processing for drug screening on living organisms on-chip

Martinus Gijs, Michaël Unser, Virginie Sophie Uhlmann, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Daniel Migliozzi

A major step for the validation of medical drugs is the screening on whole organisms, which gives the systemic information that is missing when using cellular models. Caenorhabditis elegans is a soil worm that catches the interest of researchers who study ...

SPIE Society of Photo-optical Instrumentation Engineers2019

A design of experiment approach for efficient multi-parametric drug testing using a Caenorhabditis elegans model

Martinus Gijs, Matteo Cornaglia, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia, Giuseppe Tranchida

When studying the drug effectiveness towards a target model, one should distinguish the effects of the drug itself and of all the other factors that could influence the screening outcome. This comprehensive knowledge is crucial, especially when model organ ...

2018

Integrated Microfluidic Device for Drug Studies of Early C. Elegans Embryogenesis

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele

2018

Microfluidics-enabled phenotyping of a whole population of C. elegans worms over their embryonic and post-embryonic development at single-organism resolution

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Raphaël Etienne Jean Trouillon, Maria Cristina Letizia, Vincenzo Sorrentino

2018

Reversible and long-term immobilization in a hydrogel-microbead matrix for high-resolution imaging of Caenorhabditis elegans and other small organisms

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Li Dong, Govindan Narayanan Krishnamani, Jingwei Zhang

2018

A microfluidic array for high-content screening at whole-organism resolution

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Daniel Migliozzi

A main step for the development and the validation of medical drugs is the screening on whole organisms, which gives the systemic information that is missing when using cellular models. Among the organisms of choice, Caenorhabditis elegans is a soil worm w ...

SPIE-INT SOC OPTICAL ENGINEERING2018

An Integrated Microfluidic Device For C-Elegans Early Embryogenesis Studies And Drug Assays

Thomas Lehnert, Martinus Gijs, Pierre Gönczy, Matteo Cornaglia, Li Dong, Radek Jankele, Jingwei Zhang

The Caenorhabditis elegans embryo is a widely used model for the functional analysis of dynamic cellular processes, such as chromosome segregation, cytokinesis or lineage analysis. However, the conventional embryo preparation method that relies on manually ...

Ieee2017

Microfluidic systems for high-throughput and high-content screening using the nematode Caenorhabditis elegans

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia

In a typical high-throughput drug screening (HTS) process, up to millions of chemical compounds are applied to cells cultured in well plates, aiming to find molecules that exhibit a robust dose-response, as evidenced for example by a fluorescence signal. I ...

Royal Soc Chemistry2017

C. Elegans Immobilization Using Deformable Microfluidics For In Vivo Studies Of Early Embryogenesis And Intestinal Microbiota

Thomas Lehnert, Martinus Gijs, Matteo Cornaglia, Li Dong, Xiaopeng Huang, Jingwei Zhang

The genome of the nematode Caenorhabditis elegans shows far-reaching similarities to that of humans. C. elegans is therefore considered as a powerful model organism in biological research related to humans, for instance for the study of human diseases. Suc ...

Ieee2017

A microfluidic platform for automated phenotyping during full lifespan of Caenorhabditis elegans at high-throughput

Martinus Gijs, Johan Auwerx, Matteo Cornaglia, Laurent Mouchiroud, Hüseyin Baris Atakan

We present a multiplexed and automated microfluidic platform for phenotyping of C. elegans during their full lifespan and at high-throughput. We used the fluorescent hsp-6::gfp strain of worms and performed experiments to observe the effect of four differe ...

2017

System for optical detection and imaging of sub-diffraction-limited nano-objects

Martinus Gijs, Hui Yang, Matteo Cornaglia

Nano-objects (8) with feature size smaller than the classical Abbe's diffraction limit can be optically detected via a microlens (4) array configured in a miniaturized microfluidic device. A hydrophilic microwell (3) array in a hydrophobic layer (2) is fir ...

2016

Track C. Biomedical Instrumentation & Micro and Nano Systems

Thomas Lehnert, Martinus Gijs, Johan Auwerx, Shreya Narasimhan, Matteo Cornaglia, Laurent Mouchiroud, Alexis Pierre Henri Marette, Virginija Jovaisaite, Govindan Narayanan Krishnamani, Vincenzo Sorrentino

De Gruyter2016

Microfluidic device, system and method for the study of organisms

Martinus Gijs, Matteo Cornaglia, Daniel Migliozzi

The invention discloses a microfluidic device for the culture, selection and/or analysis of sample organisms such as nematodes, as well as for other biological entities such as for instance animal embryos. The device features reservoirs, culture chambers a ...

2016