Publications de Andreas Wiederkehr

Regulation of autophagy by perilysosomal calcium: a new player in β-cell lipotoxicity

Autophagy is an essential quality control mechanism for maintaining organellar functions in eukaryotic cells. Defective autophagy in pancreatic beta cells has been shown to be involved in the progression of diabetes through impaired insulin secretion under ...

Springernature2024

SLC25A46 promotes mitochondrial fission and mediates resistance to lipotoxic stress in INS-1E insulin-secreting cells

Loïc Dayon, Andreas Wiederkehr

Glucose sensing in pancreatic D-cells depends on oxidative phosphorylation and mitochondria-derived signals that promote insulin secretion. Using mass spectrometry-based phosphoproteo-mics to search for downstream effectors of glucose-dependent signal tran ...

COMPANY BIOLOGISTS LTD2023

CDN1163, an activator of sarco/endoplasmic reticulum Ca2+ ATPase, up-regulates mitochondrial functions and protects against lipotoxicity in pancreatic β-cells

Andreas Wiederkehr

Background and Purpose: High levels of Ca2+ in the endoplasmic reticulum (ER), established by the sarco/endoplasmic reticulum Ca2+ ATPase (SERCA), are required for protein folding and cell signalling. Excessive ER Ca2+ release or decreased SERCA activity i ...

2023

Augmented mitochondrial energy metabolism is an early response to chronic glucose stress in human pancreatic beta cells

Loïc Dayon, Isabelle Chareyron, Andreas Wiederkehr, Sofia Moco

Aims/hypothesis In islets from individuals with type 2 diabetes and in islets exposed to chronic elevated glucose, mitochondrial energy metabolism is impaired. Here, we studied early metabolic changes and mitochondrial adaptations in human beta cells durin ...

2020

Encapsulation of Insulin-Secreting Cells Expressing a Genetically Encoded Fluorescent Calcium Indicator for Cell-Based Sensing In Vivo

Nicolas Bouche, Christophe Boss, Andreas Wiederkehr, Mouna Conrad, Umberto De Marchi, Federico Sizzano

The development of cell-based biosensors that give insight into cell and tissue function in vivo is an attractive technology for biomedical research. Here, the development of a cell line expressing a fluorescent calcium sensor for the study of beta-cell fu ...

Wiley2017

Advances in pancreatic islet monolayer culture on glass surfaces enable super-resolution microscopy and insights into beta cell ciliogenesis and proliferation

Jeffrey Alan Hubbell, Olivier Burri, Miriella Carmela Pasquier, Andreas Wiederkehr, Steinunn Baekkeskov Hanahan, Edward Allen Phelps, Gabriele Galliverti, Chiara Cianciaruso

A robust and reproducible method for culturing monolayers of adherent and well-spread primary islet cells on glass coverslips is required for detailed imaging studies by super-resolution and livecell microscopy. Guided by an observation that dispersed isle ...

Springer Nature2017

Illuminating redox biology using NADH- and NADPH-specific sensors

Andreas Wiederkehr

Nature Publishing Group2017

Mitochondrial Matrix Calcium Is an Activating Signal for Hormone Secretion

Kristina Schoonjans, Chikage Mataki, Andreas Wiederkehr

Mitochondrial Ca2+ signals have been proposed to accelerate oxidative metabolism and ATP production to match Ca2+-activated energy-consuming processes. Efforts to understand the signaling role of mitochondrial Ca2+ have been hampered by the inability to ma ...

Cell Press2011