Publications de Martin Ehrbar | EPFL Graph Search

Extracellular Matrix Production by Mesenchymal Stromal Cells in Hydrogels Facilitates Cell Spreading and Is Inhibited by FGF-2

In native tissues, the interaction between cells and the surrounding extracellular matrix (ECM) is reciprocal, as cells not only receive signals from the ECM but also actively remodel it through secretion of cell-derived ECM. However, very little is known ...

2020

PEG/HA Hybrid Hydrogels for Biologically and Mechanically Tailorable Bone Marrow Organoids

Martin Ehrbar, Queralt Vallmajó Martín, Nicolas Antoine Vincent Broguiere

Bone marrow (BM) organoids provide powerful tools to study the vital interplay between the BM microenvironment and resident cells. Current biomaterials, however, are limited in terms of versatility for independently studying the biochemical and biophysical ...

WILEY-V C H VERLAG GMBH2020

Smart Hydrogels for the Augmentation of Bone Regeneration by Endogenous Mesenchymal Progenitor Cell Recruitment

Matthias Lütolf, Vincent Milleret, Aline Roch, Stéphanie Elisabeth Metzger, Philipp Sebastian Lienemann, Martin Ehrbar, Olaia Maria Naveiras Torres-Quiroga, Panagiota Papageorgiou, Queralt Vallmajó Martín, Manuel Koch

The treatment of bone defects with recombinant bone morphogenetic protein-2 (BMP-2) requires high doses precluding broad clinical application. Here, a bioengineering approach is presented that strongly improves low-dose BMP-2-based bone regeneration by mob ...

WILEY2020

The NAD-Booster Nicotinamide Riboside Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance

Matthias Lütolf, Yannick Lionel Yersin, Vincent Trachsel, Bart Deplancke, Nicola Vannini, Johan Auwerx, Pedro Romero, Carlos Canto Alvarez, Martin Ehrbar, Mukul Girotra, Aurélien Laurent Jean-Charles Oggier, Dongryeol Ryu, Eija Riitta Pirinen, Olaia Maria Naveiras Torres-Quiroga, Pernille Yde Rainer, Gennady Nikitin, Vasco Ledebur de Antas de Campos, Joanna Ratajczak, Loïc Tauzin, Josefine Catharina Pedersen Tratwal, Federico Sizzano, George Coukos, Sonja Giger, Shanti Rojas-Sutterlin, Yang Li, Simone Ragusa

It has been recently shown that increased oxidative phosphorylation, as reflected by increased mitochondrial activity, together with impairment of the mitochondrial stress response, can severely compromise hematopoietic stem cell (HSC) regeneration. Here w ...

CELL PRESS2019

Label-Free Quantification Proteomics for the Identification of Mesenchymal Stromal Cell Matrisome Inside 3D Poly(Ethylene Glycol) Hydrogels

Matthias Lütolf, Yannick Robert Devaud, Martin Ehrbar

Cells modulate the functional properties of their environment by depositing extracellular matrix (ECM) proteins during biological processes in vivo and in vitro. Despite the ECMs central role in tissue formation, its quantification in hydrogels like Matrig ...

WILEY2018

The NAD plus Salvage Pathway Potently Stimulates Hematopoiesis through Increased Mitochondrial Clearance and Asymmetric Division

Matthias Lütolf, Yannick Lionel Yersin, Bart Deplancke, Nicola Vannini, Carlos Canto Alvarez, Martin Ehrbar, Mukul Girotra, Aurélien Laurent Jean-Charles Oggier, Dongryeol Ryu, Eija Riitta Pirinen, Olaia Maria Naveiras Torres-Quiroga, Pernille Yde Rainer, Gennady Nikitin, Vasco Ledebur de Antas de Campos, Joanna Ratajczak, Loïc Tauzin, Josefine Catharina Pedersen Tratwal, Federico Sizzano, George Coukos, Sonja Giger, Shanti Rojas-Sutterlin, Yang Li, Simone Ragusa

AMER SOC HEMATOLOGY2018

Engineered cell instructive matrices for fetal membrane healing

Martin Ehrbar

Iatrogenic preterm prelabour rupture of fetal membranes (iPPROM) occurs in 6-45% of the cases after fetoscopic procedures, posing a significant threat to fetal survival and well-being. The number of diagnostic and therapeutic prenatal interventions availab ...

Elsevier Sci Ltd2015

Locally controlling mesenchymal stem cell morphogenesis by 3D PDGF-BB gradients towards the establishment of an in vitro perivascular niche

Matthias Lütolf, Vincent Milleret, Yannick Robert Devaud, Philipp Sebastian Lienemann, Martin Ehrbar

The perivascular niche is a complex microenvironment containing mesenchymal stem cells (MSCs), among other perivascular cells, as well as temporally organized biochemical and biophysical gradients. Due to a lack of conclusive phenotypic markers, MSCs' iden ...

Royal Soc Chemistry2015

Long-lasting fibrin matrices ensure stable and functional angiogenesis by highly tunable, sustained delivery of recombinant VEGF(164)

Mikaël Martino, Jeffrey Alan Hubbell, Kristen Lorentz, Elena Groppa, Martin Ehrbar, Veronica Sacchi

Clinical trials of therapeutic angiogenesis by vascular endothelial growth factor (VEGF) gene delivery failed to show efficacy. Major challenges include the need to precisely control in vivo distribution of growth factor dose and duration of expression. Re ...

National Academy of Sciences2014

Electrochemical Control of the Enzymatic Polymerization of PEG Hydrogels: Formation of Spatially Controlled Biological Microenvironments

Vincent Milleret, Philipp Sebastian Lienemann, Martin Ehrbar

Wiley-Blackwell2014

3D growth factor patterned cell substrates to locally control cellular behavior

Matthias Lütolf, Vincent Milleret, Yannick Robert Devaud, Philipp Sebastian Lienemann, Martin Ehrbar

Wiley-Blackwell2014

In situ cell manipulation through enzymatic hydrogel photopatterning

Matthias Lütolf, Mikaël Martino, Jeffrey Alan Hubbell, Katarzyna Mosiewicz, Martin Ehrbar, Laura Kolb

The physicochemical properties of hydrogels can be manipulated in both space and time through the controlled application of a light beam. However, methods for hydrogel photopatterning either fail to maintain the bioactivity of fragile proteins and are thus ...

Nature Publishing Group2013

A red/far-red light-responsive bi-stable toggle switch to control gene expression in mammalian cells

Stéphanie Elisabeth Metzger, Martin Ehrbar

Growth and differentiation of multicellular systems is orchestrated by spatially restricted gene expression programs in specialized subpopulations. The targeted manipulation of such processes by synthetic tools with high-spatiotemporal resolution could, th ...

Oxford University Press2013

Proteolytic Processing Regulates Placental Growth Factor Activities

Jeffrey Alan Hubbell, Stéphanie Elisabeth Metzger, Martin Ehrbar, Manuel Koch

Placental growth factor (PlGF) is a critical mediator of blood vessel formation, yet mechanisms of its action and regulation are incompletely understood. Here we demonstrate that proteolytic processing regulates the biological activity of PlGF. Specificall ...

Amer Soc Biochemistry Molecular Biology Inc2013

Pharmacologically Controlled Protein Switch for ON-OFF Regulation of Growth Factor Activity

Philipp Sebastian Lienemann, Martin Ehrbar

The precise manipulation of growth factor signaling is central to the progress of tissue engineering. Methods for direct time-resolved activation of signaling pathways through controlled receptor dimerization have been reported; however, these suffer from ...

Nature Publishing Group2013

A Versatile Approach to Engineering Biomolecule-Presenting Cellular Microenvironments

Matthias Lütolf, Philipp Sebastian Lienemann, Martin Ehrbar

Wiley-Blackwell2013

Engineering biomimetic hydrogels for induced recruitment of mesenchymal stem cells in vitro and in vivo

Matthias Lütolf, Philipp Sebastian Lienemann, Martin Ehrbar, Olaia Maria Naveiras Torres-Quiroga

Wiley-Blackwell2012

Keynote: Engineering homing and differentiation of mesenchymal progenitor cells during bone healing

Matthias Lütolf, Philipp Sebastian Lienemann, Martin Ehrbar

Wiley-Blackwell2012

Longterm cell-demanded release of TG-VEGF for the development of mature vascular networks in vivo

Jeffrey Alan Hubbell, Daniel Eberli, Kristen Lorentz, Martin Ehrbar

Wiley-Blackwell2012

Engineering 3D cell instructive microenvironments by rational assembly of artificial extracellular matrices and cell patterning

Matthias Lütolf, Adrian Ranga, Martin Ehrbar

Engineered artificial microenvironments hold enormous potential as models to study developmental, physiological, pathological, and regenerative processes under highly defined conditions. Such platforms aim at bridging the gap between traditional in vitro 2 ...

2011