Publications de Frederik Roman Wurm

Site-Specific Polymer Attachment to HR2 Peptide Fusion Inhibitors against HIV-1 Decreases Binding Association Rates and Dissociation Rates Rather Than Binding Affinity

Harm-Anton Klok, Maarten Danial, Frederik Roman Wurm

A popular strategy for overcoming the limited plasma half-life of peptide heptad repeat 2 (HR2) fusion inhibitors against HIV-1 is conjugation with biocompatible polymers such as poly(ethylene glycol) (PEG). However, despite improved resistance to proteoly ...

2017

Squaric acid mediated bioconjugation expanded to polymers prepared by ATRP

Harm-Anton Klok, Frederik Roman Wurm

The squaric acid mediated bioconjugation reaction is an attractive approach for the amine-selective protein modification with side-chain functional polymers. This manuscript presents 3 squaric acid based atom transfer radical polymerization (ATRP) initiato ...

Royal Soc Chemistry2014

Be squared: expanding the horizon of squaric acid-mediated conjugations

Harm-Anton Klok, Frederik Roman Wurm

Squaric acid diesters can be applied as reagents to couple two amino-functional compounds. Consecutive coupling of two amines allows the synthesis of asymmetric squaric acid bisamides with either low molecular weight compounds but also biomolecules or poly ...

Royal Society of Chemistry2013

One-pot squaric acid diester mediated aqueous protein conjugation

Harm-Anton Klok, Frederik Roman Wurm

A water-soluble squaric acid dialkyl diester derivative is presented, which enables one-pot, two-step amine-selective protein conjugation reactions with (functional) amines in water. This reagent not only allows all-aqueous protein modifications, but also ...

Royal Society of Chemistry2013

Functional, Hyperbranched Polyesters Via Baylis-Hillman Polymerization

Harm-Anton Klok, Sanhao Ji, Frederik Roman Wurm

Hyperbranched polyesters are among the most common hyperbranched polymers. One of the interesting features of hyperbranched polyesters is that they contain unreacted hydroxyl and carboxylic acid groups at the linear and terminal structural units, which can ...

2012

Squaric Acid Mediated Synthesis and Biological Activity of a Library of Linear and Hyperbranched Poly(Glycerol)-Protein Conjugates

Harm-Anton Klok, Frederik Roman Wurm

Polymer-protein conjugates generated from side chain functional synthetic polymers are attractive because they can be easily further modified with, for example, labeling groups or targeting ligands. The residue specific modification of proteins with side c ...

American Chemical Society2012

Squaric Acid Mediated Chemoselective PEGylation of Proteins: Reactivity of Single-Step-Activated alpha-Amino Poly(ethylene glycol)s

Harm-Anton Klok, Frederik Roman Wurm

The covalent attachment of poly(ethylene glycol) (PEG) to therapeutically active proteins (PEGylation) has become an important method to deal with the pharmacological difficulties of these polypeptides, such as short body-residence times and immunogenicity ...

Wiley-V C H Verlag Gmbh2012

PEG-based Multifunctional Polyethers with Highly Reactive Vinyl-Ether Side Chains for Click-Type Functionalization

Frederik Roman Wurm

Introduction of highly reactive vinyl ether moieties along a poly(ethylene-glycol) (PEG) backbone has been realized by copolymerization of the novel epoxide monomer ethoxy vinyl glycidyl ether (EVGE) with ethylene oxide (EO). A series of copolymers with va ...

American Chemical Society2011

Arginine-specific protein modification using alpha-oxo-aldehyde functional polymers prepared by atom transfer radical polymerization

Harm-Anton Klok, Maxime Ayer, Marc Gauthier, Justyna Kowal, Frederik Roman Wurm

The residue-specific modification of peptides and proteins is a powerful strategy for preparing biomolecular-synthetic polymer conjugates with advanced properties. This manuscript aims at expanding the present toolbox of residue-selective protein modificat ...

2011

Polymers Interfacing with Biology

Harm-Anton Klok, Frederik Roman Wurm

The development of various controlled radical polymerization techniques as well as site- and residue specific strategies to modify peptides/proteins with synthetic polymers have made polymer chemistry a powerful tool to address materials problems at the bi ...

2011

From an Epoxide Monomer Toolkit to Functional PEG Copolymers With Adjustable LCST Behavior

Frederik Roman Wurm

The lower critical solution temperature (LCST) behavior of novel poly(ethylene glycol) (PEG)-based copolymers bearing multiple functional groups, obtained by anionic ring-opening (co)polymerization (AROP), has been investigated. Variable comonomer ratios o ...

2011

Rapid Access to Polyfunctional Lipids with Complex Architecture via Oxyanionic Ring-Opening Polymerization

Frederik Roman Wurm

Polymer-coated liposomes, particularly poly(ethylene glycol) (PEG)-substituted liposomes, have emerged as long-circulating carrier systems for drug delivery and diagnostic purposes. A rapid synthesis of three different types of multifunctional lipids with ...

American Chemical Society2011

"Functional Poly(ethylene glycol)": PEG-Based Random Copolymers with 1,2-Diol Side Chains and Terminal Amino Functionality

Frederik Roman Wurm

A series of poly(ethylene glycol-co-isopropylidene glyceryl glycidyl ether) (P(EO-co-IGG)) random copolymers with different fractions of 1,2-isopropylidene glyceryl glycidyl ether (IGG) units was synthesized. After acidic hydrolysis a new type of "function ...

American Chemical Society2010