Publications de Xiao Shen | EPFL Graph Search

Improved process conditions for increasing expression of MHC class II protein from a stable Drosophila S2 cell line

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

2018

Studies on fluid dynamics of the flow field and gas transfer in orbitally shaken tubes

Florian Maria Wurm, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Likuan Zhu

Orbitally shaken cylindrical bioreactors [OrbShake bioreactors (OSRs)] without an impeller or sparger are increasingly being used for the suspension cultivation of mammalian cells. Among small volume OSRs, 50-mL tubes with a ventilated cap (OSR50), origina ...

American Chemical Society2017

Disposable orbitally shaken TubeSpin bioreactor 600 for Sf9 cell cultivation in suspension

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen, Dominique Terry Monteil Jr, Giulia Tontodonati

Disposable orbitally shaken TubeSpin bioreactor 600 tubes (TS600s) were recently developed for the bench-scale cultivation of animal cells in suspension. Here we compared batch cultures of Sf9 insect cells in TS600s, spinner flasks, and shake flasks. Super ...

Elsevier2016

A simple plasmid-based transient gene expression method using High Five cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Virginie Bachmann, Xiao Shen

The High Five (H5) cell line, derived from the lepidopteran Trichoplusia ni, is one of the major insect cell hosts for the production of recombinant proteins using the baculovirus expression vector system (BEVS). Here, we describe a simple polyethylenimine ...

Elsevier2015

Virus-free transient protein production in Sf9 cells

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

A method for virus-free transient gene expression from suspension-adapted Sf9 insect cells was developed with the gene of interest being expressed from a plasmid carrying the homologous region 5 enhancer (hr5) and the immediate early 1 (ie1) promoter from ...

Elsevier2014

Cell Compatible Arginine Containing Cationic Polymer: One-Pot Synthesis and Preliminary Biological Assessment

Jutta Christine Wandrey, Lucia Baldi Unser, Xiao Shen, Nino Zavradashvili, Tamar Memanishvili

Synthetic cationic polymers are of interest as both nonviral vectors for intracellular gene delivery and antimicrobial agents. For both applications synthetic polymers containing guanidine groups are of special interest since such kind of organic compounds ...

Springer India2014

Development of plasmid-based expression technology for rapid and sustained production of recombinant proteins in insect cells

Xiao Shen

Due to the high demand for heterogeneous protein production in insect cells, we have established efficient methods for plasmid-based transient gene expression (TGE) in various insect cell lines, including commonly used Spodoptera cell lines Sf9 and HighFiv ...

EPFL2014

Study of the improved Sf9 transient gene expression process

Florian Maria Wurm, Lucia Baldi Unser, David Hacker, Xiao Shen

Insect cells have been widely used for the production of recombinant proteins using recombinant baculovirus for gene delivery [1]. To simplify protein production in insect cells, we have previously described a method, based on transient gene expression (TG ...

2013

Transient transfection of insect Sf-9 cells in TubeSpin® bioreactor 50 tubes

Florian Maria Wurm, David Hacker, Qiuling Xie, Xiao Shen

2011