Publications de Elena Tobolkina

Electrostatic spray ionization from 384-well microtiter plates for mass spectrometry analysis based enzyme assay and drug metabolism screening

Hubert Girault, Liang Qiao, Elena Tobolkina, Tzu-En Lin, Xiaoqin Zhong, Yan Deng, Emna Belghith

We have realized the direct ionization of samples from wells of microtiter plates under atmospheric conditions for mass spectrometry analysis without any liquid delivery system or any additional interface. The microtiter plate is a commercially available 3 ...

American Chemical Society2017

Electrostatic Spray Ionization Method

Romain Sartor, Yu Lu, Liang Qiao, Elena Tobolkina

In an electrostatic spray ionization method for spraying a liquid layer from an insulating plate 2, the plate is arranged between two electrodes 1, 4. A constant high voltage power supply 3 is provided and an electric circuit is used to charge and discharg ...

2015

Standard addition strip for quantitative electrostatic spray ionization mass spectrometry analysis: Determination of caffeine in drinks

Hubert Girault, Christophe Roussel, Liang Qiao, Elena Tobolkina

Standard addition strips were prepared for the quantitative determination of caffeine in different beverages by electrostatic spray ionisation mass spectrometry (ESTASI-MS). The gist of this approach is to dry spots of caffeine solutions with different con ...

Elsevier2014

New analytical tools combining gel electrophoresis and mass spectrometry

Elena Tobolkina

Proteomics has been one of the main projects challenging biological and analytical chemists for many years. The separation, identification and quantification of all the proteins expressed within biological systems remain the main objectives of proteomics. ...

EPFL2014

Electrostatic Spray Ionization Mass Spectrometry Imaging

Hubert Girault, Horst Vogel, Horst Pick, Liang Qiao, Elena Tobolkina, Andreas Stephan Lesch, Alexandra Bondarenko, Xiaoqin Zhong

Imaging samples on a surface by mass spectrometry (MS) requires the combination of MS detection with a scanning mode that enables localized desorption and ionization and/or detection of sample analytes with good spatial resolution. We have developed a new ...

Amer Chemical Soc2014

Proteins/peptides purification by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels

Hubert Girault, Fernando Cortes Salazar, Liang Qiao, Elena Tobolkina

Purification and desalting of protein and peptide samples by a three-well OFFGEL electrophoresis with immobilized ultra narrow pH gradient gels is proposed as a fast preparative strategy for proteomics. The gist of this strategy is to separate the proteins ...

Royal Society of Chemistry2014

Coupling Isoelectric Focusing Gel Electrophoresis to Mass Spectrometry by Electrostatic Spray Ionisation

Hubert Girault, Liang Qiao, Elena Tobolkina

Gel electrophoresis has been used for decades as a high-resolution separation technique for proteins and protein isomers, but limited in the coupling with MS because of low throughput and poor automaticity compared with LC-MS. In this work, we have develop ...

American Chemical Society2013

Electrostatic-spray ionization mass spectrometry sniffing for perfume fingerprinting

Hubert Girault, Liang Qiao, Elena Tobolkina

RATIONALE: The perfume market is growing significantly, and it is easy to find imitative fragrances of probably all types of perfume. Such imitative fragrances are usually of lower quality than the authentic ones, creating a possible threat for perfume com ...

Wiley-Blackwell2013

Electrostatic-Spray Ionization Mass Spectrometry

Hubert Girault, Romain Sartor, Yu Lu, Liang Qiao, Elena Tobolkina, Natalia Gasilova

An electrostatic-spray ionization (ESTASI) method has been used for mass spectrometry (MS) analysis of samples deposited in or on an insulating substrate. The ionization is induced by a capacitive coupling between an electrode and the sample. In practice, ...

Amer Chemical Soc2012

Segmented Field OFFGEL® Electrophoresis

Hubert Girault, Julien Maillard, Fernando Cortes Salazar, Dmitry Momotenko, Elena Tobolkina

A multielectrode setup for protein OFFGEL electrophoresis that significantly improves protein separation efficiency has been developed. Here, the electric field is applied by segments between seven electrodes connected in series to six independent power su ...

Wiley-Blackwell2012