Publications de Randy Patrick Carney

Selective Localization of Hierarchically Assembled Particles to Plasma Membranes of Living Cells

Francesco Stellacci, Randy Patrick Carney

Particles that preferentially partition to a specific cellular subunit, such as the nucleus, mitochondria, or the cytoskeleton, are of relevance to a number of applications, including drug delivery, genetic manipulation, or self-assembly. Here, hierarchica ...

WILEY-V C H VERLAG GMBH2019

Structure-Property Relationships of Amphiphilic Nanoparticles That Penetrate or Fuse Lipid Membranes

Davide Demurtas, Francesco Stellacci, Randy Patrick Carney, Paulo Henrique Jacob Silva, Zekiye Pelin Güven, Ye Yang, Ahmet Bekdemir

2018

Superparamagnetic Nanoparticles as High Efficiency Magnetic Resonance Imaging T-2 Contrast Agent

Kurt Schenk, Henrik Moodysson Rønnow, Arash Alahgholipour Omrani, Francesco Stellacci, Randy Patrick Carney, Paulo Henrique Jacob Silva, Tamara Michael Carney, Ping Huang, Lin Yang

Nanoparticle-based magnetic resonance imaging T-2 negative agents are of great interest, and much effort is devoted to increasing cell loading capability while maintaining low cytotoxicity. Herein, two classes of mixed-ligand protected magnetic-responsive, ...

American Chemical Society2017

Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months

Francesco Stellacci, Randy Patrick Carney, Paulo Henrique Jacob Silva, Valeria Cagno

Up to 80% of the cost of vaccination programmes is due to the cold chain problem (that is, keeping vaccines cold). Inexpensive, biocompatible additives to slow down the degradation of virus particles would address the problem. Here we propose and character ...

Nature Publishing Group2016

A silica-based magnetic platform decorated with mixed ligand gold nanoparticles: a recyclable catalyst for esterification reactions

Francesco Stellacci, Randy Patrick Carney, Elif Ertem Bekdemir, Emma-Rose Janecek

A novel and convenient synthetic strategy for the preparation of magnetically responsive silica nanospheres decorated with mixed ligand protected gold nanoparticles is described. Gold nanoparticles are attached to the silica surface via stable amide bond f ...

Royal Society of Chemistry2016

Influence of the glycocalyx and plasma membrane composition on amphiphilic gold nanoparticle association with erythrocytes

Francesco Stellacci, Randy Patrick Carney, Paulo Henrique Jacob Silva, Ye Yang, Ahmet Bekdemir

Erythrocytes are attractive as potential cell-based drug carriers because of their abundance and long life-span in vivo. Existing methods for loading drug cargos into erythrocytes include hypotonic treatments, electroporation, and covalent attachment onto ...

Royal Society of Chemistry2015

Isolation and Characterization of Monodisperse Core Shell Nanoparticle Fractions

Raffaele Mezzenga, Francesco Stellacci, Randy Patrick Carney

Monodispersity is a key property to control the self-assembly of colloidal particles, and is typically reached after fine-tuning of the synthesis conditions. Monodisperse particle fractions can also be separated from polydisperse suspensions via ultracentr ...

American Chemical Society2015

Effects of Surface Compositional and Structural Heterogeneity on Nanoparticle-Protein Interactions: Different Protein Configurations

Francesco Stellacci, Randy Patrick Carney

As nanoparticles (NPs) enter into biological systems, they are immediately exposed to a variety and concentration of proteins. The physicochemical interactions between proteins and NPs are influenced by the surface properties of the NPs. To identify the ef ...

American Chemical Society2014

Enhancing Radiotherapy by Lipid Nanocapsule-Mediated Delivery of Amphiphilic Gold Nanoparticles to Intracellular Membranes

Francesco Stellacci, Randy Patrick Carney, Ye Yang

Amphiphilic gold nanoparticles (amph-NPs), composed of gold cores surrounded by an amphiphilic mixed organic ligand shell, are capable of embedding within and traversing lipid membranes. Here we describe a strategy using crosslink-stabilized lipid nanocaps ...

American Chemical Society2014

A general mechanism for intracellular toxicity of metal-containing nanoparticles

Francesco Stellacci, Randy Patrick Carney

The assessment of the risks exerted by nanoparticles is a key challenge for academic, industrial, and regulatory communities worldwide. Experimental evidence points towards significant toxicity for a range of nanoparticles both in vitro and in vivo. Worldw ...

Royal Society of Chemistry2014

Protein-nanoparticle interactions: the effects of surface compositional and structural heterogeneity are scale dependent

Francesco Stellacci, Randy Patrick Carney

Nanoparticles (NPs) in the biological environment are exposed to a large variety and concentration of proteins. Proteins are known to adsorb in a 'corona' like structure on the surface of NPs. In this study, we focus on the effects of surface compositional ...

Royal Society of Chemistry2013

Colloidal Stability of Self-Assembled Mono layer-Coated Gold Nanoparticles: The Effects of Surface Compositional and Structural Heterogeneity

Francesco Stellacci, Randy Patrick Carney

Surface heterogeneity plays an important role in controlling colloidal phenomena. This study investigated the self-aggregation and bacterial adsorption of self-assembled monolayer coated gold nanoparticles (AuNPs) with different surface compositional and s ...

American Chemical Society2013

Electrical Method to Quantify Nanoparticle Interaction with Lipid Bilayers

Francesco Stellacci, Kislon Voitchovsky, Randy Patrick Carney, Paulo Henrique Jacob Silva, Tamara Michael Carney

Understanding as well as rapidly screening the interaction of nanoparticles with cell membranes is of central importance for biological applications such as drug and gene delivery. Recently, we have shown that "striped" mixed-monolayer-coated gold nanopart ...

American Chemical Society2013

Sensing Single Mixed-Monolayer Protected Gold Nanoparticles by the alpha-Hemolysin Nanopore

Francesco Stellacci, Randy Patrick Carney

Gold nanoparticles are widely used in various applications in fields including chemistry, engineering, biology, medicine, and electronics. These materials can be synthesized and modified with ligands containing different functional groups. Among nanopartic ...

American Chemical Society2013

Effect of Particle Diameter and Surface Composition on the Spontaneous Fusion of Monolayer-Protected Gold Nanoparticles with Lipid Bilayers

Francesco Stellacci, Randy Patrick Carney, Ye Yang

Anionic, monolayer-protected gold nanoparticles (AuNPs) have been shown to nondisruptively penetrate cellular membranes. Here, we show that a critical first step in the penetration process is potentially the fusion of such AuNPs with lipid bilayers. Free e ...

American Chemical Society2013

Cell-Penetrating Nanoparticles : Characterization and Function

Randy Patrick Carney

For targeted cellular therapy using both synthetic and natural biomaterials, there is a need for better understanding of cell penetration mechanisms. Recently, nanoparticles (NPs) coated with mixed-ligand self-assembled monolayers exhibiting order in their ...

EPFL2013

Dynamic Cellular Uptake of Mixed-Monolayer Protected Nanoparticles

Francesco Stellacci, Randy Patrick Carney, Marie Mueller, Tamara Michael Carney

Nanoparticles (NPs) are gaining increasing attention for potential application in medicine; consequently, studying their interaction with cells is of central importance. We found that both ligand arrangement and composition on gold nanoparticles play a cru ...

American Vacuum Society2012

Synthesis and Characterization of Janus Gold Nanoparticles

Francesco Stellacci, Randy Patrick Carney, Quy Ong Khac, Javier Reguera Gomez

When gold nanoparticles are coated with binary mixtures of dislike ligand molecules, separation in the ligand shell occurs; if the particles are smaller than a threshold size the separation is solely enthalpy driven leading to the spontaneous formation of ...

Wiley2012

Determination of nanoparticle size distribution together with density or molecular weight by 2D analytical ultracentrifugation

Francesco Stellacci, Randy Patrick Carney, Jiwon Kim

Nanoparticles are finding many research and industrial applications, yet their characterization remains a challenge. Their cores are often polydisperse and coated by a stabilizing shell that varies in size and composition. No single technique can character ...

Nature Research2011

Oligonucleotide Delivery by Cell-Penetrating "Striped" Nanoparticles

Francesco Stellacci, Randy Patrick Carney

Gold nanoparticles coated with homogeneous (MUS) or "striped" (MUS-OT) ligand shells can be conjugated with double or single stranded DNA, and particles with either ligand configuration effectively carry DNA into melanoma tumor cells. When endocytosis is i ...

Wiley-VCH Verlag GmbH2011