Publications de Yuhei Takado | EPFL Graph Search

Association between Brain and Plasma Glutamine Levels in Healthy Young Subjects Investigated by MRS and LC/MS

Both glutamine (Gln) and glutamate (Glu) are known to exist in plasma and brain. However, despite the assumed relationship between brain and plasma, no studies have clarified the association between them. Proton magnetic resonance spectroscopy (MRS) was se ...

2019

Hyperpolarized C-13 Magnetic Resonance Spectroscopy Reveals the Rate-Limiting Role of the Blood-Brain Barrier in the Cerebral Uptake and Metabolism of L-Lactate in Vivo

Arnaud Comment, Sylvain Eric Lengacher, Bernard Lanz, Mor-Miri Mishkovsky, Tian Cheng, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Hikari Ananda Infinity Yoshihara

The dynamics of L-lactate transport across the blood-brain barrier (BBB) and its cerebral metabolism are still subject to debate. We studied lactate uptake and intracellular metabolism in the mouse brain using hyperpolarized C-13 magnetic resonance spectro ...

2018

Direct Monitoring of γ-Glutamyl Transpeptidase Activity In Vivo Using a Hyperpolarized (13) C-Labeled Molecular Probe

Arnaud Comment, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Emine Can, Hikari Ananda Infinity Yoshihara

The γ-glutamyl transpeptidase (GGT) enzyme plays a central role in glutathione homeostasis. Direct detection of GGT activity could provide critical information for the diagnosis of several pathologies. We propose a new molecular probe, γ-Glu-[1-(13) C]Gly, ...

Wiley-Blackwell2016

Imaging the time-integrated cerebral metabolic activity with subcellular resolution through nanometer-scale detection of biosynthetic products deriving from (13)C-glucose

Arnaud Comment, Graham Knott, Yuhei Takado, Anders Meibom, Stéphane Laurent Escrig, Mojgan Masoodi

Glucose is the primary source of energy for the brain but also an important source of building blocks for proteins, lipids, and nucleic acids. Little is known about the use of glucose for biosynthesis in tissues at the cellular level. We demonstrate that l ...

Elsevier2015

Imaging liver and brain glycogen metabolism at the nanometer scale

Arnaud Comment, Graham Knott, Yuhei Takado, Anders Meibom, Stéphane Laurent Escrig, Mojgan Masoodi

In mammals, glycogen synthesis and degradation are dynamic processes regulating blood and cerebral glucose-levels within a well-defined physiological range. Despite the essential role of glycogen in hepatic and cerebral metabolism, its spatiotemporal distr ...

Elsevier Science Bv2015

Hyperpolarized 13C lactate as a substrate for in vivo metabolic studies in skeletal muscle

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Hikari Ananda Infinity Yoshihara

Resting skeletal muscle has a preference for the oxidation of lipids compared to carbohydrates and a shift towards carbohydrate oxidation is observed with increasing exercise. Lactate is not only an end product in skeletal muscle but also an important meta ...

2014

Hyperpolarized 13C lactate as a substrate for in vivo metabolic studies in skeletal muscle

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Hikari Ananda Infinity Yoshihara

2014

Over 35% liquid-state 13C polarization via dissolution dynamic nuclear polarization at 7 T and 1 K with ubiquitous nitroxyl radicals

Arnaud Comment, Riccardo Balzan, Tian Cheng, Yuhei Takado, Andrea Capozzi

The most versatile method to increase liquid-state 13C NMR sensitivity is dissolution dynamic nuclear polarization. The use of trityl radicals is usually required to obtain very large 13C polarization via this technique. We herein demonstrate that up to 35 ...

Royal Soc Chemistry2013

Hyperpolarized [1-13C]lactate metabolism in rat skeletal muscle in vivo

Arnaud Comment, Rolf Gruetter, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Hikari Ananda Infinity Yoshihara

2013

Over 35% of liquid-state 13C polarization enhanced by TEMPOL via high field dissolution dynamic nuclear polarization

Arnaud Comment, Riccardo Balzan, Tian Cheng, Yuhei Takado, Andrea Capozzi

2013

Hyperpolarization without persistent radicals for in vivo real-time metabolic imaging

Arnaud Comment, Christophe Roussel, Mor-Miri Mishkovsky, Tian Cheng, Tim Rolf Eichhorn, Yuhei Takado, Jean-Noël Hyacinthe, Najat Salameh, Andrea Capozzi

Hyperpolarized substrates prepared via dissolution dynamic nuclear polarization have been proposed as magnetic resonance imaging (MRI) agents for cancer or cardiac failure diagnosis and therapy monitoring through the detection of metabolic impairments in v ...

National Academy of Sciences2013

BDPA as a polarizing agent for in vivo hyperpolarized MR experiment

Arnaud Comment, Rolf Gruetter, Mor-Miri Mishkovsky, Tian Cheng, Josefina Adriana Maria Bastiaansen, Yuhei Takado, Najat Salameh, Emine Can

2012

In vivo real-time metabolic studies of mice brain and skeletal muscle at 9.4T

Arnaud Comment, Rolf Gruetter, Mor-Miri Mishkovsky, Tian Cheng, Josefina Adriana Maria Bastiaansen, Yuhei Takado

2011