Publications de Krisztian Homicsko

HPV16-Expressing Tumors Release Multiple IL-1 Ligands to Orchestrate Systemic Immunosuppression Whose Disruption Enables Efficacy of a Therapeutic Vaccine

Douglas Hanahan, Krisztian Homicsko, Jeremy Gilles Louis Guillot

It is well-established that symptomatic cancers evade immune destruction by coalescing tumor microenvironments to suppress adaptive immunity. Additionally, mouse models of cervical and other cancers have revealed a capability of tumors to systemically indu ...

American Association for Cancer Research (AACR)2025

Probing the killing potency of tumor-infiltrating lymphocytes on microarrayed colorectal cancer tumoroids

Matthias Lütolf, Damien Saugy, Nathalie Brandenberg, François Réal Rivest, Sylke Höhnel, Krisztian Homicsko, Nicolas Antoine Vincent Broguiere, Devanjali Dutta

Immunotherapy has emerged as a new standard of care for certain cancer patients with specific cellular and molecular makeups. However, there is still an unmet need for ex vivo models able to readily assess the effectiveness of immunotherapeutic treatments ...

2024

ACMSD INHIBITION REDUCES HEPATIC INFLAMMATION AND FIBROSIS IN A MURINE THERMONEUTRALITY MODEL OF NASH

Johan Auwerx, Richardus Houtkooper, Krisztian Homicsko, Maroun Bou Sleiman, Xiaoxu Li, Sandra Rodriguez Lopez, Jia Liu, Keno Strotjohann

Lippincott Williams & Wilkins2023

INHIBITING SARM1 IS MORE EFFICACIOUS THAN NICOTINAMIDE RIBOSIDE SUPPLEMENTATION FOR THE TREATMENT OF A MOUSE MODEL WITH NAFLD/NASH

Johan Auwerx, Richardus Houtkooper, Krisztian Homicsko, Maroun Bou Sleiman, Xiaoxu Li, Sandra Rodriguez Lopez, Jia Liu, Keno Strotjohann

Lippincott Williams & Wilkins2023

Bispecific PD1-IL2v and anti-PD-L1 break tumor immunity resistance by enhancing stem-like tumor- reactive CD8+T cells and reprogramming macrophages

Douglas Hanahan, Krisztian Homicsko, Stephan Wullschleger, Mélanie Louise Tichet, Agnieszka Alicja Chryplewicz

Immunotherapies have shown remarkable, albeit tumor-selective, therapeutic benefits in the clinic. Most pa-tients respond transiently at best, highlighting the importance of understanding mechanisms underlying resistance. Herein, we evaluated the effects o ...

CELL PRESS2023

Cancer cell autophagy, reprogrammed macrophages, and remodeled vasculature in glioblastoma triggers tumor immunity

Julie Laetitia Scotton, Douglas Hanahan, Ksenya Shchors, Krisztian Homicsko, Mélanie Louise Tichet, Agnieszka Alicja Chryplewicz

Glioblastoma (GBM) is poorly responsive to therapy and invariably lethal. One conceivable strategy to circumvent this intractability is to co-target distinctive mechanistic components of the disease, aiming to concomitantly disrupt multiple capabilities re ...

2022

Aberrant hyperexpression of the RNA binding protein FMRP in tumors mediates immune evasion

Nadine Fournier, Douglas Hanahan, Krisztian Homicsko, Mohammad Sadegh Saghafinia, Dalya Ataca, Qiqun Zeng, Mélanie Louise Tichet, Yuqing Xie, Agnieszka Alicja Chryplewicz, Fleuriane Marie Tryskele Laurene Gallean, Jeremy Gilles Louis Guillot, Simge Yücel, Lucine Christe, Peng Zhang, Inti Zlobec

Many human cancers manifest the capability to circumvent attack by the adaptive immune system. In this work, we identified a component of immune evasion that involves frequent up-regulation of fragile X mental retardation protein (FMRP) in solid tumors. FM ...

American Association for the Advancement of Science2022

Cancer Cells Retrace a Stepwise Differentiation Program during Malignant Progression

Douglas Hanahan, Krisztian Homicsko, Stephan Wullschleger, Mohammad Sadegh Saghafinia, Iacovos Michael, Giovanni Ciriello

Pancreatic neuroendocrine tumors (PanNET) comprise two molecular subtypes, relatively benign islet tumors (IT) and invasive, metastasis-like primary (MLP) tumors. Until now, the origin of aggressive MLP tumors has been obscure. Herein, using multi-omics ap ...

AMER ASSOC CANCER RESEARCH2021

The combination of stereotactic radiosurgery with immune checkpoint inhibition or targeted therapy in melanoma patients with brain metastases: a retrospective study

Olivier Michielin, Alexandre Wicky, Krisztian Homicsko, Constantin Tuleasca, Filipe Amândio Brandão Sanches Vong Martins

Background Evidence pointing to a synergistic effect of stereotactic radiosurgery (SRS) with concurrent immunotherapy or targeted therapy in patients with melanoma brain metastases (BM) is increasing. We aimed to analyze the effect on overall survival (OS) ...

SPRINGER2020

High-throughput automated organoid culture via stem-cell aggregation in microcavity arrays

Gerardo Turcatti, Matthias Lütolf, Nathalie Brandenberg, Fabien Kuttler, Krisztian Homicsko, Nikolche Gjorevski, George Coukos, Camilla Carla Alma Ceroni

Stem-cell-derived epithelial organoids are routinely used for the biological and biomedical modelling of tissues. However, the complexity, lack of standardization and quality control of stem cell culture in solid extracellular matrices hampers the routine ...

NATURE PUBLISHING GROUP2020

Trametinib Induces the Stabilization of a Dual GNAQ p.Gly48Leu-and FGFR4 p.Cys172Gly-Mutated Uveal Melanoma. The Role of Molecular Modelling in Personalized Oncology

Olivier Michielin, Alexandre Wicky, Krisztian Homicsko

We report a case of an uveal melanoma patient with GNAQ p.Gly48Leu who responded to MEK inhibition. At the time of the molecular analysis, the pathogenicity of the mutation was unknown. A tridimensional structural analysis showed that G alpha(q) can adopt ...

2020

Unsorted single-cell RNA sequencing profiles of metastatic melanoma patients reveal the heterogeneity of melanoma-associated fibroblasts

Marco Matter, Douglas Hanahan, Krisztian Homicsko

OXFORD UNIV PRESS2019

T cell–induced CSF1 promotes melanoma resistance to PD1 blockade

Sina Nassiri, Krisztian Homicsko, Natacha Anne-Sophie Bordry, Michele De Palma, Laure Tillé, Esther Danenberg, Christophe Martignier, George Coukos

Colony-stimulating factor 1 (CSF1) is a key regulator of monocyte/macrophage differentiation that sustains the protumorigenic functions of tumor-associated macrophages (TAMs). We show that CSF1 is expressed in human melanoma, and patients with metastatic m ...

2018

Proton pump inhibitors negatively impact survival of PD-1 inhibitor based therapies in metastatic melanoma patients

Douglas Hanahan, Krisztian Homicsko

OXFORD UNIV PRESS2018

Tumor lymphangiogenesis promotes T cell infiltration and potentiates immunotherapy in melanoma

Melody Swartz, Olivier Michielin, Lambert Charles François Potin, Marcela Rincón-Restrepo, Amanda Waite Lund, Laura Jeanbart, Douglas Hanahan, Sylvie Hauert, Krisztian Homicsko, Natacha Anne-Sophie Bordry, Christopher Claude Giuseppe Tremblay, Manuel Andreas Fankhauser, Maria Anna Sofia Broggi, Elodie Da Costa

In melanoma, vascular endothelial growth factor-C (VEGF-C) expression and consequent lymphangiogenesis correlate with metastasis and poor prognosis. VEGF-C also promotes tumor immunosuppression, suggesting that lymphangiogenesis inhibitors may be clinicall ...

Amer Assoc Advancement Science2017

Compact solid-state CMOS single-photon detector array for in vivo NIR fluorescence lifetime oncology measurements

Claudio Bruschini, Edoardo Charbon, Krisztian Homicsko, François Eric Powolny, Riccardo Sinisi, Harald Arjan Robert Homulle, David Li

In near infrared fluorescence-guided surgical oncology, it is challenging to distinguish healthy from cancerous tissue. One promising research avenue consists in the analysis of the exogenous fluorophores' lifetime, which are however in the (sub-)nanosecon ...

2016

Combine and Conquer: Double CTLA-4 and PD-1 Blockade Combined with Whole Tumor Antigen Vaccine Cooperate to Eradicate Tumors

Krisztian Homicsko, George Coukos

2016

The consensus molecular subtypes of colorectal cancer

Paolo Angelino, Mauro Delorenzi, Douglas Hanahan, Anguraj Sadanandam, Krisztian Homicsko, Sarah Gerster, Xin Wang

Colorectal cancer (CRC) is a frequently lethal disease with heterogeneous outcomes and drug responses. To resolve inconsistencies among the reported gene expression-based CRC classifications and facilitate clinical translation, we formed an international c ...

Nature Publishing Group2015

Targeting Programmed Cell Death 1 in Ovarian Cancer

Krisztian Homicsko, George Coukos

Amer Soc Clinical Oncology2015

Time-resolved imaging system for fluorescence-guided surgery with lifetime imaging capability

Claudio Bruschini, Edoardo Charbon, Douglas Hanahan, Krisztian Homicsko, François Eric Powolny, Riccardo Sinisi, Harald Arjan Robert Homulle

We present a single-photon camera for fluorescence imaging, with a time resolution better than 100ps, capable of providing both intensity and lifetime images. the camera was fabricated in standard CMOS technology. With this FluoCam we show the possibility ...

Spie-Int Soc Optical Engineering2014